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海洋生命科学与技术（英文）
海洋生命科学与技术（英文）
海洋生命科学与技术（英文）/Journal Marine Life Science & TechnologyCSCDSCI
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    A chromosome-level genome of electric catfish(Malapterurus electricus)provided new insights into order Siluriformes evolution

    Meiru LiuYue SongSuyu ZhangLili Yu...
    1-14页
    查看更多>>摘要：The electric catfish(Malapterurus electricus),belonging to the family Malapteruridae,order Siluriformes(Actinopterygii:Ostariophysi),is one of the six branches that has independently evolved electrical organs.We assembled a 796.75 Mb M.electricus genome and anchored 88.72％sequences into 28 chromosomes.Gene family analysis revealed 295 expanded gene families that were enriched on functions related to glutamate receptors.Convergent evolutionary analyses of electric organs among different lineage of electric fishes further revealed that the coding gene of rho guanine nucleotide exchange factor 4-like(arhgef4),which is associated with G-protein coupled receptor(GPCR)signaling pathway,underwent adaptive parallel evolution.Gene identification suggests visual degradation in catfishes,and an important role for taste in environ-mental adaptation.Our findings fill in the genomic data for a branch of electric fish and provide a relevant genetic basis for the adaptive evolution of Siluriformes.
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    Mating behaviors in ovoviviparous black rockfish(Sebastes schlegelii):molecular function of prostaglandin E2 as both a hormone and pheromone

    Likang LyuYijia YaoSongyang XieXiaojie Wang...
    15-30页
    查看更多>>摘要：Prostaglandins(PGs)are profound hormones in teleost sexual behavior,especially in mating.PGs act as pheromones that affect the olfactory sensory neurons of males,inducing the initiation of a series of mating behaviors.However,the molecu-lar mechanism by which PGs trigger mating behavior in ovoviviparous teleosts is still unclear.In the present study,we employed the ovoviviparous black rockfish(Sebastes schlegelii),an economically important marine species whose reproduc-tive production is limited by incomplete fertilization,as a model species.The results showed that when the dose of PGE2 was higher than 10 nmol/L,a significant(P＜0.05)increase in mating behaviors was observed.Dual-fluorescence in situ hybridization indicated that PGE2 could fire specific neurons in different brain regions and receptor cells in the olfactory sac.After combining with specific neurons in the central nervous system(CNS),a series of genes related to reproduction are activated.The intracerebroventricular administration of PGE2 significantly increased lhb levels(P＜0.05)in both sexes.Moreover,steroidogenesis in gonads was also affected,inducing an increase(P＜0.05)in E2 levels in males and T levels in females.PGE2 levels were also increased significantly(P＜0.05)in both sexes.The present study revealed that PGE2 can activate mating behavior in black rockfish in both hormone and pheromone pathways,leading to variations in sex steroid levels and activation of reproductive behaviors.Our results provide not only novel insight into the onset of mating behaviors in ovoviviparous teleosts but also solutions for the incomplete fertilization caused by natural mating in cage aquaculture.
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    From germline genome to highly fragmented somatic genome:genome-wide DNA rearrangement during the sexual process in ciliated protists

    Liping LyuXue ZhangYunyi GaoTengteng Zhang...
    31-49页
    查看更多>>摘要：Genomes are incredibly dynamic within diverse eukaryotes and programmed genome rearrangements(PGR)play important roles in generating genomic diversity.However,genomes and chromosomes in metazoans are usually large in size which prevents our understanding of the origin and evolution of PGR.To expand our knowledge of genomic diversity and the evolutionary origin of complex genome rearrangements,we focus on ciliated protists(ciliates).Ciliates are single-celled eukaryotes with highly fragmented somatic chromosomes and massively scrambled germline genomes.PGR in ciliates occurs extensively by removing massive amounts of repetitive and selfish DNA elements found in the silent germline genome dur-ing development of the somatic genome.We report the partial germline genomes of two spirotrich ciliate species,namely Strombidium cf.sulcatum and Halteria grandinella,along with the most compact and highly fragmented somatic genome for S.cf.sulcatum.We provide the first insights into the genome rearrangements of these two species and compare these features with those of other ciliates.Our analyses reveal:(1)DNA sequence loss through evolution and during PGR in S.cf.sulcatum has combined to produce the most compact and efficient nanochromosomes observed to date;(2)the compact,transcriptome-like somatic genome in both species results from extensive removal of a relatively large number of shorter germline-specific DNA sequences;(3)long chromosome breakage site motifs are duplicated and retained in the somatic genome,revealing a complex model of chromosome fragmentation in spirotrichs;(4)gene scrambling and alternative pro-cessing are found throughout the core spirotrichs,offering unique opportunities to increase genetic diversity and regulation in this group.
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    New perspectives on the genetic structure of dotted gizzard shad(Konosirus punctatus)based on RAD-seq

    Ying PengYifan LiuJiasheng LiKun Zhang...
    50-67页
    查看更多>>摘要：To maintain,develop and rationally utilize marine organisms,understanding their genetic structure and habitat adaptation pattern is necessary.Konosirus punctatus,which is a commercial fish species inhabiting the Indo-west Pacific Ocean,has shown an obvious annual global capture and aquaculture production decline due to climate changes and human activities.In the present study,restriction-site associated DNA sequencing(RAD-seq)was used to describe its genome-wide single nucleotide polymorphisms panel(SNPs).Among 146 individuals collected at nine locations scattered in China,Korea and Japan,a set of 632,090 SNPs were identified.Population genetic analysis showed that K.punctatus individuals were divided into two significant genetic clusters.Meanwhile,potential genetic differentiation between northern and southern popula-tion of K.punctatus was found.Treemix results indicated that gene flow existed among sampling locations of K.punctatus,especially from southern Japan to others.Moreover,candidate genes associated with habitat adaptations of K.punctatus were identified,which are involved in diverse physiological processes of K.punctatus including growth and development(e.g.,KIDINS220,PAN3),substance metabolism(e.g.,PGM5)and immune response(e.g.,VAV3,CCT7,HSPA12B).Our findings may aid in understanding the possible mechanisms for the population genetic structure and local adaptation of K.punctatus,which is beneficial to establish the management and conservation units of K.punctatus,guiding the rational use of resources,with reference significance for a profound understanding of the adaptative mechanisms of other marine organ-isms to the environment.
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    Incorporating mesopelagic fish into the evaluation of conservation areas for marine living resources under climate change scenarios

    Shuhao LiuYang LiuKatharina TeschkeMark A.Hindell...
    68-83页
    查看更多>>摘要：Mesopelagic fish(meso-fish)are central species within the Southern Ocean(SO).However,their ecosystem role and adap-tive capacity to climate change are rarely integrated into protected areas assessments.This is a pity given their importance as crucial prey and predators in food webs,coupled with the impacts of climate change.Here,we estimate the habitat distribution of nine meso-fish using an ensemble model approach(MAXENT,random forest,and boosted regression tree).Four climate model simulations were used to project their distribution under two representative concentration pathways(RCP4.5 and RCP8.5)for short-term(2006-2055)and long-term(2050-2099)periods.In addition,we assess the ecologi-cal representativeness of protected areas under climate change scenarios using meso-fish as indicator species.Our models show that all species shift poleward in the future.Lanternfishes(family Myctophidae)are predicted to migrate poleward more than other families(Paralepididae,Nototheniidae,Bathylagidae,and Gonostomatidae).In comparison,lanternfishes were projected to increase habitat area in the eastern SO but lose area in the western SO;the opposite was projected for species in other families.Important areas(IAs)of meso-fish are mainly distributed near the Antarctic Peninsula and East Antarctica.Negotiated protected area cover 23％of IAs at present and 38％of IAs in the future(RCP8.5,long-term future).Many IAs of meso-fish still need to be included in protected areas,such as the Prydz Bay and the seas around the Antarctic Peninsula.Our results provide a framework for evaluating protected areas incorporating climate change adaptation strate-gies for protected areas management.
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    Tetramic acid-motif natural products from a marine fungus Tolypocladium cylindrosporum FB06 and their anti-Parkinson activities

    Yuna JungChaesun KwonTaeyeon KimJin Woo Lee...
    84-92页
    查看更多>>摘要：Tetramic acid-containing natural products are attracting significantly increasing attention from biologists and chemists due to their intriguing structures and biological activities.In the present study,two new tetramic acid alkaloids tolypyridone I(1)and tolypyridone J(2),together with five known ones(3-7),were isolated from cultures of a marine fungus Tolypocla-dium cylindrosporum FB06 isolate obtained from a marine sediment in Beaufort sea of North Alaska.Their structures were elucidated using 1D,2D NMR,and HRESIMS.Their configurations were established on the basis of 1H coupling constants,ROESY correlations and DP4 calculations.Compound 2 was isolated as mixtures of rotational isomers with C-3 to C-7 axis between 4-hydroxy-2-pyridone and 1-ethyl-3,5-dimethylcyclohexane,hindering rotation.In our unbiased screening to dis-cover neuroprotective compounds in an in vitro Parkinson's disease(PD)model,SH-SY5Y dopaminergic cells were treated with isolated compounds followed by treatment with 1-methyl-4-phenylpyridinium(MPP+),a parkinsonian neurotoxin.Among tested compounds,F-14329(7)significantly protected cells from MPP+-induced cytotoxicity.MPP+-mediated cell death is known to be related to the regulation of Bcl-2 family proteins,specifically the down-regulation of anti-apoptotic Bcl-2 and the up-regulation of pro-apoptotic Bax levels.Treatment with 2 mmol/L of MPP+for 24 h significantly reduced Bcl-2 levels compared to control treated with vehicle.However,treatment with F-14329(7)attenuated such reduction.This study demonstrates that tetramic acid-motif compounds could be potential lead compounds for treating PD.
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    The hydrophobic cluster on the surface of protein is the key structural basis for the SDS-resistance of chondroitinase VhChlABC

    Juanjuan SuHao WuChengying YinFengchao Zhang...
    93-101页
    查看更多>>摘要：The application of chondroitinase requires consideration of the complex microenvironment of the target.Our previous research reported a marine-derived sodium dodecyl sulfate(SDS)-resistant chondroitinase VhChlABC.This study further investigated the mechanism of VhChlABC resistance to SDS.Focusing on the hydrophobic cluster on its strong hydrophilic surface,it was found that the reduction of hydrophobicity of surface residues Ala181,Met182,Met183,Ala184,Vai185,and Ile305 significantly reduced the SDS resistance and stability.Molecular dynamics(MD)simulation and molecular docking analysis showed that I305G had more conformational flexibility around residue 305 than wild type(WT),which was more conducive to SDS insertion and binding.The affinity of A181G,M182A,M183A,V185A and I305G to SDS was significantly higher than that of WT.In conclusion,the surface hydrophobic microenvironment composed of six residues was the structural basis for SDS resistance.This feature could prevent the binding of SDS and the destruction of hydrophobic packaging by increasing the rigid conformation of protein and reducing the binding force of SDS-protein.The study provides a new idea for the rational design of SDS-resistant proteins and may further promote chondroitinase research in the targeted therapy of lung diseases under the pressure of pulmonary surfactant.
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    Griseofulvin enantiomers and bromine-containing griseofulvin derivatives with antifungal activity produced by the mangrove endophytic fungus Nigrospora sp.QQYB1

    Ge ZouWencong YangTao ChenZhaoming Liu...
    102-114页
    查看更多>>摘要：Marine microorganisms have long been recognized as potential sources for drug discovery.Griseofulvin was one of the first antifungal natural products and has been used as an antifungal agent for decades.In this study,12 new griseofulvin derivatives[(±)-1-2,(+)-3,(±)-4,10-12,and 14-15]and two new griseofulvin natural products(9 and 16)together with six known analogues[(-)-3,5-8,and 13]were isolated from the mangrove-derived fungus Nigrospora sp.QQYB1 treated with 0.3％NaCl or 2％NaBr in rice solid medium.Their 2D structures and absolute configurations were established by extensive spectroscopic analysis(1D and 2D NMR,HRESIMS),ECD spectra,computational calculation,DP4+analysis,and X-ray single-crystal diffraction.Compounds 1-4 represent the first griseofulvin enantiomers with four absolute con-figurations(2S,6'S;2R,6'R;2S,6'R;2R,6'S),and compounds 9-12 represent the first successful production of brominated griseofulvin derivatives from fungi via the addition of NaBr to the culture medium.In the antifungal assays,compounds 6 and 9 demonstrated significant inhibitory activities against the fungi Colletotrichum truncatum,Microsporum gypseum,and Trichophyton mentagrophyte with inhibition zones varying between 28 and 41 mm(10 pg/disc).The structure-activity relationship(SAR)was analyzed,which showed that substituents at C-6,C-7,C-6'and the positions of the carbonyl and double bond of griseofulvin derivatives significantly affected the antifungal activity.
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    A chitosan-based antibacterial hydrogel with injectable and self-healing capabilities

    Rui ChenYanan HaoSecundo FrancescoXiangzhao Mao...
    115-125页
    查看更多>>摘要：The presence of bacteria directly affects wound healing.Chitosan-based hydrogel biomaterials are a solution as they offer advantages for wound-healing applications due to their strong antimicrobial properties.Here,a double-cross-linking chitosan-based hydrogel with antibacterial,self-healing,and injectable properties is reported.Thiolated chitosan was successfully prepared,and the thiolated chitosan molecules were cross-linked by Ag-S coordination to form a supramolecular hydrogel.Subsequently,the amine groups in the thiolated chitosan covalently cross-linked with genipin to further promote hydrogel formation.In vitro experimental results indicate that hydrogel can release Ag+over an extended time,achieving an antibac-terial rate of over 99％against Escherichia coli and Staphylococcus aureus.Due to the reversible and dynamic feature of Ag-S coordination,an antibacterial hydrogel exhibited injectable and self-healing capabilities.Additionally,the hydrogel showed excellent biocompatibility and biodegradability.
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    Genome-resolved metagenomics of Venice Lagoon surface sediment bacteria reveals high biosynthetic potential and metabolic plasticity as successful strategies in an impacted environment

    Elisa BanchiErwan CorrePaola Del NegroMauro Celussi...
    126-142页
    查看更多>>摘要：Bacteria living in sediments play essential roles in marine ecosystems and deeper insights into the ecology and bio-geochemistry of these largely unexplored organisms can be obtained from'omics'approaches.Here,we characterized metagenome-assembled-genomes(MAGs)from the surface sediment microbes of the Venice Lagoon(northern Adriatic Sea)in distinct sub-basins exposed to various natural and anthropogenic pressures.MAGs were explored for biodiver-sity,major marine metabolic processes,anthropogenic activity-related functions,adaptations at the microscale,and biosynthetic gene clusters.Starting from 126 MAGs,a non-redundant dataset of 58 was compiled,the majority of which(35)belonged to(Alpha-and Gamma-)Proteobacteria.Within the broad microbial metabolic repertoire(including C,N,and S metabolisms)the potential to live without oxygen emerged as one of the most important features.Mixotrophy was also found as a successful lifestyle.Cluster analysis showed that different MAGs encoded the same metabolic pat-terns(e.g.,C fixation,sulfate oxidation)thus suggesting metabolic redundancy.Antibiotic and toxic compounds resist-ance genes were coupled,a condition that could promote the spreading of these genetic traits.MAGs showed a high biosynthetic potential related to antimicrobial and biotechnological classes and to organism defense and interactions as well as adaptive strategies for micronutrient uptake and cellular detoxification.Our results highlighted that bacteria living in an impacted environment,such as the surface sediments of the Venice Lagoon,may benefit from metabolic plasticity as well as from the synthesis of a wide array of secondary metabolites,promoting ecosystem resilience and stability toward environmental pressures.
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