首页期刊导航|中国海洋大学学报（自然科学英文版）
期刊信息/Journal information
中国海洋大学学报（自然科学英文版）
中国海洋大学学报（自然科学英文版）

文圣常（院士）

季刊

1672-5182

xbywb@ouc.edu.cn

0532-82032408

266003

山东省青岛市鱼山路5号

中国海洋大学学报（自然科学英文版）/Journal Journal of Ocean University of ChinaCSCDCSTPCD北大核心SCI
查看更多>>创刊宗旨：我刊及时反映和报道海洋科学与工程、海洋环境科学与工程、海洋气象、海洋药物、海洋遥感、海洋信息系统与管理工程、水产、湖沼等领域的最新研究成果。论文栏目设置：研究论文、技术报告、研究快报、高新技术与信息跟踪、特约评介、综述等。读者对象：国内海洋及相关学科领域的科技人员、教师和管理人员，主要是国际海洋科学界的同行，以英语为语言工具的读者。
正式出版
收录年代

    Characterization of a Novel Esterase Belonging to Family V from Marinobacter flavimaris

    HE JingjingZHANG YunhuiWU LeileiWANG Yaru...
    221-232页
    查看更多>>摘要：Lipolytic enzymes have attracted enormous attentions because of their ability in ester hydrolysis,ester synthesis,trans-esterification and other biochemical reactions.Bacteria are important sources of lipolytic enzymes applied in industry.Here,a novel lipolytic enzyme encoded by esterase gene est1347 was identified in Marinobacter flavimaris WLL162,and was purified and cha-racterized.The lipolytic enzyme Est1347 consisted of 312 amino acid residues and a 21-amino-acids N-terminal signal peptide with a predicted molecular weight of 34.2 kDa.It belongs to family V of bacterial lipolytic enzymes based on the amino acid sequence ho-mology analysis.Est1347 is a mesophilic and alkali-resistant enzyme with the highest activity at 45℃and pH 8.5;it is stable at tem-peratures below 50℃and pH 7.5-11.0.Est1347 showed a preference for middle-length chain substrate p-NPC10 and a wide range of other substrates.The Km,Vmax,Kcat and Kcat/Km values of Est1347 for p-NPC10 in pH 8.5 at 45℃were 0.9411 mmol L-1,1285 μmol min-1 mg-1,698.91 s-1 and 743.65 s-1(mmol L-1)-1,respectively.It is also tolerant to the metal ions,organic solvents and deter-gents.In conclusion,the esterase Est1347 laid a foundation for further study of bacterial lipolytic enzyme family V.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Effects of Biostimulation-Bioaugmentation on Coastal Microbial Community in an in situ Mesocosm System

    YUAN FangzhengZHAO YangyongDAI YulaiYANG Wen...
    233-246页
    查看更多>>摘要：Globally,various types of pollution affect coastal waters as a result of human activities.Bioaugmentation and biostimu-lation are effective methods for treating water pollution.However,few studies have explored the response of coastal prokaryotic and eukaryotic communities to bioaugmentation and biostimulation.Here,a 28-day outdoor mesocosm experiment with two treatments(bioaugmentation-A and combined treatment of bioaugmentation and biostimulation-AS)and a control(untreated-C)were carried out.The experiment was conducted in Meishan Bay to explore the composition,dynamics,and co-occurrence patterns of prokaryotic and eukaryotic communities in response to the A and AS using 16S rRNA and 18S rRNA gene amplicon sequencing.After treatment,Gammaproteobacteria and Epsilonproteobacteria were significantly increased in group AS compared to group C,while Flavobac-teriia and Saprospirae were significantly reduced.Dinoflagellata was significantly reduced in AS compared to C,while Chrysophyta was significantly reduced in both AS and A.Compared to C,the principal response curve analyses of the prokaryotic and eukaryotic communities both showed an increasing trend followed by a decreasing trend for AS.Furthermore,the trends of prokaryotic and eu-karyotic communities in group A were similar to those in group AS compared with group C,but AS changed them more than A did.According to the species weight table on principal response curves,a significant increase was observed in beneficial bacteria in pro-karyotic communities,such as Rhodobacterales and Oceanospirillales,along with a decrease in autotrophs in eukaryotic communities,such as Chrysophyta and Diatom.Topological properties of network analysis reveal that A and AS complicate the interactions between the prokaryotic and eukaryotic communities.Overall,these findings expand our understanding of the response pattern of the bioaug-mentation and biostimulation on coastal prokaryotic and eukaryotic communities.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Numerical Simulation of the Settling Flux of Biodeposition in a Bay with Cage Culture Through Similarity Theory and a Simplified Pollution Source

    LIU YaoCHEN YifanGE Changzi
    247-254页
    查看更多>>摘要：The settling flux of biodeposition affects the environmental quality of cage culture areas and determines their environ-mental carrying capacity.Simple and effective simulation of the settling flux of biodeposition is extremely important for determining the spatial distribution of biodeposition.Theoretically,biodeposition in cage culture areas without specific emission rules can be simplified as point source pollution.Fluent is a fluid simulation software that can simulate the dispersion of particulate matter simply and efficiently.Based on the simplification of pollution sources and bays,the settling flux of biodeposition can be easily and effec-tively simulated by Fluent fluid software.In the present work,the feasibility of this method was evaluated by simulation of the set-tling flux of biodeposition in Maniao Bay,Hainan Province,China,and 20 sampling sites were selected for determining the settling fluxes.At sampling sites P1,P2,P3,P4,P5,Z1,Z2,Z3,Z4,A1,A2,A3,A4,B1,B2,C1,C2,C3 and C4,the measured settling fluxes of biodeposition were 26.02,15.78,10.77,58.16,6.57,72.17,12.37,12.11,106.64,150.96,22.59,11.41,18.03,7.90,19.23,7.06,11.84,5.19 and 2.57 g d-1 m-2,respectively.The simulated settling fluxes of biodeposition at the corresponding sites were 16.03,23.98,8.87,46.90,4.52,104.77,16.03,8.35,180.83,213.06,39.10,17.47,20.98,9.78,23.25,7.84,15.90,6.06 and 1.65 g d-1 m-2,respectively.There was a positive correlation between the simulated settling fluxes and measured ones(R = 0.94,P = 2.22×10-9<0.05),which implies that the spatial differentiation of biodeposition flux was well simulated.Moreover,the posterior difference ratio of the simulation was 0.38,and the small error probability was 0.94,which means that the simulated results reached an acceptable level from the perspective of relative error.Thus,if nonpoint source pollution is simplified to point source pollution and open waters are simplified based on similarity theory,the setting flux of biodeposition in the open waters can be simply and effectively simulated by the fluid simulation software Fluent.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    Photoperiod Mediates the Effects of Temperature and Light Intensity on the Proliferation of Ulva prolifera

    JIANG JiananYU YanyanCHEN YiliLI Yahe...
    255-263页
    查看更多>>摘要：In order to study the complex effects of photoperiod,temperature,and light intensity on the spore maturation and release number of Ulva prolifera,we cultured thalli segment(2-3 mm)under three different photoperiods(L:D = 12:12,14:10 and 10:14),temperature(15℃(LT),25℃(MT)and 30℃(HT))and light intensity(100,200 and 400 μmol m-2 s-1,noted as LL,ML and HL,respectively)conditions.Then the maturation time,spore release number and chlorophyll fluorescence were analyzed.The results suggested that:1)The spore maturation time was accelerated by higher temperature or higher light intensity from 62 h to 36 h,and changes in day length accelerated the spore maturation to a certain extent as compared with 12:12 light/dark cycle;2)Higher light intensity significantly decreased the chlorophyll fluorescence(Fv'/Fm',NPQ,rETRmax and α)of the mature reproductive segment under 30℃with 12:12 light/dark cycle.But when in the other photoperiods(10:14 and 14:10 conditions),the inhibitory effects of high light intensity were alleviated significantly;3)The optimum condition for the spore maturation and release was 12:12 light/dark cycle,25℃,400 μmol m-2 s-1,with both shorter and longer photoperiod reducing the spore release number;4)Higher light intensity significantly increased the spore release number under 25℃,but these effects were alleviated by 30℃treatment.This study is the first attempt to elucidate the coincidence effects of photoperiod,temperature and light intensity on the reproduction of Ulva,which would help to reveal the mechanism of the rapid proliferation of green tide.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普

    The Variation of Plankton Community Structure in Artificial Reef Area and Adjacent Waters in Haizhou Bay

    GAO ShikeSHI YixiLU YananZHANG Shuo...
    264-276页
    查看更多>>摘要：Plankton are an important component of marine protected areas(MPAs),and its communities would require much smaller interpatch distances to ensure connection among MPAs.According to the survey from MPAs dominated by artificial reefs and adjacent waters(estuary area(EA),aquaculture area(AA),artificial reef area(ARA),natural area(NA)and comprehensive ef-fect area(CEA))in Haizhou Bay in spring and autumn,we analyzed phyto-zooplankton composition,abundance and biomass,and correlation with hydrologic variables to gain information about the forces that structure the plankton.The results showed that the dominant zooplankton were copepods(spring,98.9%;autumn,94.2%),while the phytoplankton were mainly composed of Bacil-lariophyta(spring,61.8%;autumn,95.6%).The RDA results showed that temperature,salinity and depth highly associated with the distribution and composition of plankton species among the habitats than other factors in spring;temperature,Chla and DO had the strongest influence in autumn.The zooplankton in the ARA and AA ecosystems basically contained the same species as those in other habitats,and each habitat also exhibited a relatively unique combination of plankton species.The structures of the EA zoo-plankton in spring and the EA phytoplankton in both seasons were much different than other habitats,which may have been caused by factors such as currents and tides.We concluded that there exists similarity of the plankton community between artificial reef area and adjacent waters,whereas the EAs may be relatively independent systems.Therefore,these interaction between plankton commu-nity should be considered when designing MPA networks,and ocean circulations should be considered more than the environmental factors.
      原文链接:
    • NETL
    • NSTL
    • 万方数据
    • 维普