查看更多>>摘要:As an efficient immunostimulant,chitosan oligosaccharide(COS)can enhance the immunity of teleosts;however,the un-derlying molecular mechanisms still require elucidation.Competing endogenous RNAs(ceRNAs)are vital regulators in the immune response,but their roles in half-smooth tongue sole(Cynoglossus semilaevis)remain unclear.In this study,for the first time,we studied whole-transcriptome expression profiles and analyzed ceRNA networks in peripheral blood leukocytes of half-smooth tongue sole treated with COS.A total of 19 circRNAs(DE-circRNAs),18 miRNAs(DE-miRNAs)and 50 previously identified lncRNAs(DE-lncRNAs)were differentially expressed after COS stimulation.The DE-lncRNAs and DE-miRNAs targeted numerous immunity-re-lated genes,and were enriched in important pathways,including MAPK and Toll-like receptor signaling pathways,suggesting the im-munoregulatory roles of COS.Furthermore,we constructed circRNA-miRNA-mRNA and lncRNA-miRNA-mRNA regulatory net-works using DE-circRNAs,DE-miRNAs,DE-lncRNAs,and DE-mRNAs.Additionally,a ceRNA network with immunity-related DE-mRNAs was constructed,showing that 3 DE-circRNAs,12 DE-lncRNAs,and 29 DEGs exhibited crosstalk through 9 DE-miRNAs.Intriguingly,a DE-miRNA in the ceRNA network,miR-144-3p,was targeted by DE-lncRNA tnrc6a,and negatively regulated the genes of inhibitor of nuclear factor kappa B kinases(IKKs)(ikbkg,ikbkb,and ikbip)and c3ar1.Ikbkg,ikbkb,and c3ar1 were sig-nificantly up-regulated in macrophages stimulated by LPS.It could be inferred that ncRNAs participated in the immune and inflam-matory response by acting as ceRNAs after COS stimulation in teleosts.These findings indicate that COS could enhance the im-munity of teleosts by regulating ncRNAs,and lay the foundation for further practical application of COS in aquaculture.