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生物设计与制造（英文）
生物设计与制造（英文）

季刊

2096-5524

生物设计与制造（英文）/Journal Bio-Design and Manufacturing CSCD北大核心SCI
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    Biofabrication and biomanufacturing in Ireland and the UK

    Jack F.MurphyMartha LavelleLisa AsciakRoss Burdis...
    825-856页
    查看更多>>摘要：As we navigate the transition from the Fourth to the Fifth Industrial Revolution,the emerging fields of biomanufacturing and biofabrication are transforming life sciences and healthcare.These sectors are benefiting from a synergy of synthetic and engineering biology,sustainable manufacturing,and integrated design principles.Advanced techniques such as 3D bioprinting,tissue engineering,directed assembly,and self-assembly are instrumental in creating biomimetic scaffolds,tissues,organoids,medical devices,and biohybrid systems.The field of biofabrication in the United Kingdom and Ireland is emerging as a pivotal force in bioscience and healthcare,propelled by cutting-edge research and development.Concentrating on the production of biologically functional products for use in drug delivery,in vitro models,and tissue engineering,research institutions across these regions are dedicated to innovating healthcare solutions that adhere to ethical standards while prioritising sustainability,affordability,and healthcare system benefits.
      原文链接:
    • 万方数据
    • 维普

    3D bioprinting of tumor models and potential applications

    Huaixu LiYang QiaoXingliang DaiHaotian Tian...
    857-888页
    查看更多>>摘要：Cancer is the most common cause of human mortality and has created an unbridgeable health gap due to its unrestrained aberrant proliferation,rapid growth,metastasis,and high heterogeneity.Conventional two-dimensional cell culture and animal models for tumor diagnostic and therapeutic studies have extremely low clinical translation rates due to their intrinsic limitations.Appropriate tumor models are therefore required for cancer research.Engineered human three-dimensional(3D)cancer models stand out for their ability to better replicate the spatial organization,cellular resources,and microenvironmental features(e.g.,hypoxia,necrosis,and delayed proliferation)of actual human tumors.Further,the fabrication of these models can be achieved by an emerging technology known as 3D bioprinting,which allows for the fabrication of living structures by precisely regulating the spatial distribution of cells,biomolecules,and matrix components.The aim of this paper is to review the current technologies and bioinks associated with 3D bioprinted cancer models for glioma,breast,liver,intestinal,cervical,ovarian,and neuroblastoma,as well as,advances in the applications of 3D bioprinted-based tumor models in the fields of tumor microenvironment,tumor vascularization,tumor stem cells,tumor resistance and therapeutic drug screening,tumorimmunotherapy,and precision medicine.
      原文链接:
    • 万方数据
    • 维普

    Hierarchical structures on platinum-iridium substrates enhancing conducting polymer adhesion

    Linze LiChangqing JiangLuming Li
    889-898页
    查看更多>>摘要：Conducting polymers(CPs),including poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate)(PEDOT:PSS),are promis-ing coating materials for neural electrodes.However,the weak adhesion of CP coatings to substrates such as platinum-iridium is a significant challenge that limits their practical application.To address this issue,we used femtosecond laser-prepared hierarchical structures on platinum-iridium(Pt-Ir)substrates to enhance the adhesion of PEDOT:PSS coatings.Next,we used cyclic voltammetry(CV)stress and accelerated aging tests to evaluate the stability of both drop cast and electrodeposited PEDOT:PSS coatings on Pt-Ir substrates,both with and without hierarchical structures.Our results showed that after 2000 CV cycles or five weeks of aging at 60℃,the morphology and electrochemical properties of the coatings on the Pt-Ir substrates with hierarchical structures remained relatively stable.In contrast,we found that smooth Pt-Ir substrate surfaces caused delamination of the PEDOT:PSS coating and exhibited both decreased charge storage capacity and increased impedance.Overall,enhancing the stability of PEDOT:PSS coatings used on common platinum-iridium neural electrodes offers great potential for improving their electrochemical performance and developing new functionalities.
      原文链接:
    • 万方数据
    • 维普

    Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release

    Joel Yupanqui MielesCian VyasEvangelos DaskalakisMohamed Hassan...
    899-925页
    查看更多>>摘要：Wounds pose a risk to the skin,our body's primary defence against infections.The rise of antibiotic resistance has prompted the development of novel therapies.RO-101® is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide(H2O2),a reactive oxygen species,directly to the wound bed.In this study,electrospinning was used to incorporate RO-101® into a polyvinyl alcohol(PVA)sub-micron fibrous mesh that can act as a delivery agent,achieve a sustained release profile,and provide a barrier against infection.Adequate incorporation of this gel into sub-micron fibres was confirmed via nuclear magnetic resonance spectroscopy.Furthermore,scanning electron microscopy exhibited smooth and uniform meshes with diameters in the 200-500 nm range.PVA/RO-101 electrospun meshes generated H2O2 in concentrations exceeding 1 mM/(g·mL)(1 mM=1 mmol/L)after 24 h,and the role of sterilisation on H2O2 release was evaluated.PVA/RO-101 meshes exhibited antimicrobial activity against both Gram-positive Staphylococcus aureus(S.aureus)and Gram-negative Pseudomonas aeruginosa(P.aeruginosa)bacteria,achieving viable count reductions of up to 1 log unit CFU/mm2(CFU:colony-forming units).Moreover,these meshes were capable of disrupting biofilm formation,even against multidrug-resistant organisms such as methicillin-resistant S.aureus(MRSA).Furthermore,increasing the RO-101® concentration resulted in higher H2O2 production and an enhanced antimicrobial effect,while fibroblast cell viability and proliferation tests showed a concentration-dependent response with high cytocompatibility at low RO-101® concentrations.This study therefore demon-strates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings.
      原文链接:
    • 万方数据
    • 维普

    Sustainable and untethered soft robots created using printable and recyclable ferromagnetic fibers

    Wei TangYidan GaoZeyu DongDong Han...
    926-937页
    查看更多>>摘要：Integrated printing of magnetic soft robots with complex structures using recyclable materials to achieve sustainability of the soft robots remains a persistent challenge.Here,we propose a kind of ferromagnetic fibers that can be used to print soft robots with complex structures.These ferromagnetic fibers are recyclable and can make soft robots sustainable.The ferromagnetic fibers based on thermoplastic polyurethane(TPU)/NdFeB hybrid particles are extruded by an extruder.We use a desktop three-dimensional(3D)printer to demonstrate the feasibility of printing two-dimensional(2D)and complex 3D soft robots.These printed soft robots can be recycled and reprinted into new robots once their tasks are completed.Moreover,these robots show almost no difference in actuation capability compared to prior versions and have new functions.Successful applications include lifting,grasping,and moving objects,and these functions can be operated untethered wirelessly.In addition,the locomotion of the magnetic soft robot in a human stomach model shows the prospect of medical applications.Overall,these fully recyclable ferromagnetic fibers pave the way for printing and reprinting sustainable soft robots while also effectively reducing e-waste and robotics waste materials,which is important for resource conservation and environmental protection.
      原文链接:
    • 万方数据
    • 维普

    3D-bioprinted tri-layered cellulose/collagen-based drug-eluting fillers for the treatment of deep tunneling wounds

    Mano GovindharajNoura Al HashimiSoja S.SomanJiarui Zhou...
    938-954页
    查看更多>>摘要：Tunneling wounds create passageways underneath the skin surface with varying sizes and shapes and can have twists and turns,making their treatment extremely difficult.Available wound care solutions only cater to superficial wounds,and untreated tunneling wounds pose major health concerns.This study aims to fulfill this challenge by fabricating tunnel wound fillers(TWFs)made of natural polymers that mimic the dermal extracellular matrix.In this study,cellulose microfibers(CMFs)derived from banana stem and fish skin-derived collagen were used to formulate bio-inks with varying CMF contents(25,50,and 75 mg).Tri-layered(CMFs,primary and secondary collagen coatings),drug-eluting(Baneocin),and cell-laden(human mesenchymal stem cells)TWFs were three-dimensional(3D)-printed and extensively characterized.CMFs showed the most suitable theological properties for 3D printing at 50 mg concentration.The Alamar Blue data showed significantly increased cell proliferation from Day 1 to Day 7,and scratch tests used to evaluate in vitro wound healing revealed that the best coverage of the wound area was achieved using CMFs in combination with collagen and alginate.Finally,the TWF showed promising capability and tunability in terms of wound shape and size upon testing on a chicken tissue model.The results demonstrate the tremendous potential of TWFs in treating deep tunneling wounds with unique advantages,such as patient-specific customization,good wound exudate absorption capability while releasing wound healing drugs,and the inclusion of stem cells for accelerated healing and tissue regeneration.
      原文链接:
    • 万方数据
    • 维普

    Light/pH dual controlled drug release"nanocontainer"alleviates tumor hypoxia for synergistic enhanced chemotherapy,photodynamic therapy and chemodynamic therapy

    Shihe LiuXin ZhangZhimin BaiYibo Yang...
    955-971页
    查看更多>>摘要：Photodynamic therapy(PDT)has significant advantages in treating primary tumors.However,the hypoxic tumor microenvi-ronment hinders the generation of sufficient reactive oxygen species during PDT to effectively kill tumor cells,further greatly limiting the applications of PDT in cancer treatment.Herein,we reported a temperature/pH dual controlled drug delivery system LPC@PCN@PDA/Fe3+-AS1411 based on a porous coordination network(PCN(Mn))coated with polydopamine(PDA)and modified with an aptamer AS1411.β-lapachone(LPC)was loaded inside the PCN(Mn)framework,and Fe3+was attached to the surface of the PDA coating.These nanoparticles(NPs)exhibited excellent multimodal cancer therapeutic effects and tumor targeting ability with their photo-and chemodynamic properties.The therapeutic effect can be enhanced by the production of sufficient oxygen by the internal hydrogen peroxide,which improves the photodynamic effect of the photo-sensitizer PCN(Mn)and the chemotherapy effect of β-lapachone.Notably,the conversion of Fe2+to Fe3+in the tumor cells exerts the Fenton effect,which generates hydroxyl radicals that cause lipid peroxidation in tumor cells and induce apoptosis,thus enhancing the chemodynamic therapeutic effect.In vitro and in vivo experiments revealed that the NPs demonstrated specific tumor targeting,excellent inhibition effect on tumor growth,and biocompatibility.Together,our findings can help develop an intelligent multifunctional therapeutic nanoplatform for cancer therapy.
      原文链接:
    • 万方数据
    • 维普

    Integrated nanoporous electroporation and sensing electrode array for total dynamic time-domain cardiomyocyte membrane resealing assessment

    Weiqin ShengYing LiChunlian QinZhonghai Zhang...
    972-982页
    查看更多>>摘要：Intracellular electrophysiological research is vital for biological and medical research.Traditional planar microelectrode arrays(MEAs)have disadvantages in recording intracellular action potentials due to the loose cell-electrode interface.To investigate intracellular electrophysiological signals with high sensitivity,electroporation was used to obtain intracellular recordings.In this study,a biosensing system based on a nanoporous electrode array(NPEA)integrating electrical perforation and signal acquisition was established to dynamically and sensitively record the intracellular potential of cardiomyocytes over a long period of time.Moreover,nanoporous electrodes can induce the protrusion of cell membranes and enhance cell-electrode inter-facial coupling,thereby facilitating effective electroporation.Electrophysiological signals over the entire recording process can be quantitatively and segmentally analyzed according to the signal changes,which can equivalently reflect the dynamic evolution of the electroporated cardiomyocyte membrane.We believe that the low-cost and high-performance nanoporous biosensing platform suggested in this study can dynamically record intracellular action potential,evaluate cardiomyocyte electroporation,and provide a new strategy for investigating cardiology pharmacological science.
      原文链接:
    • 万方数据
    • 维普

    Cav3.3-mediated endochondral ossification in a three-dimensional bioprinted GelMA hydrogel

    Zhi WangXin WangYang HuangJunjun Yang...
    983-999页
    查看更多>>摘要：The growth plate(GP)is a crucial tissue involved in skeleton development via endochondral ossification(EO).The bone organoid is a potential research model capable of simulating the physiological function,spatial structure,and intercellular communication of native GPs.However,mimicking the EO process remains a key challenge for bone organoid research.To simulate this orderly mineralization process,we designed an in vitro shCav3.3 ATDC5-loaded gelatin methacryloyl(GelMA)hydrogel model and evaluated its bioprintability for future organoid construction.In this paper,we report the first demonstration that the T-type voltage-dependent calcium channel(T-VDCC)subtype Cav3.3 is dominantly expressed in chondrocytes and is negatively correlated with the hypertrophic differentiation of chondrocytes during the EO process.Furthermore,Cav3.3 knockdown chondrocytes loaded with the GelMA hydrogel successfully captured the EO process and provide a bioink capable of constructing layered and orderly mineralized GP organoids in the future.The results of this study could therefore provide a potential target for regulating the EO process and a novel strategy for simulating it in bone organoids.
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    • 万方数据

    Electrospinning/3D printing-integrated porous scaffold guides oral tissue regeneration in beagles

    Li YuanChen YuanJiawei WeiShue Jin...
    1000-1017页
    查看更多>>摘要：The combined use of guided tissue/bone regeneration(GTR/GBR)membranes and bone filling grafts represents a classical therapy for guiding the regeneration and functional reconstruction of oral soft and hard tissues.Nevertheless,due to its displacement and poor mechanical support,bone meal is not suitable for implantation in the case of insufficient cortical bone support and large dimensional defects.The combination of GTR/GBR membrane with a three-dimensional(3D)porous scaffold may offer a resolution for the repair and functional reconstruction of large soft and hard tissue defects.In this study,a novel integrated gradient biodegradable porous scaffold was prepared by bonding a poly(lactic-co-glycolic acid)(PLGA)/fish collagen(FC)electrospun membrane(PFC)to a 3D-printed PLGA/nano-hydroxyapatite(HA)(PHA)scaffold.The consistency of the composition(PLGA)ensured strong interfacial bonding between the upper fibrous membrane and the lower 3D scaffold.In vitro cell experiments showed that the PFC membrane(upper layer)effectively prevented the unwanted migration of L929 cells.Further in vivo investigations with an oral soft and hard tissue defect model in beagles revealed that the integrated scaffold effectively guided the regeneration of defective oral tissues.These results suggest that the designed integrated scaffold has great potential for guiding the regeneration and reconstruction of large oral soft and hard tissues.
      原文链接:
    • 万方数据
    • 维普