Electrospun polyvinyl alcohol fibres incorporating an antimicrobial gel for enzymatically controlled reactive oxygen species release

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外文摘要：Wounds pose a risk to the skin,our body's primary defence against infections.The rise of antibiotic resistance has prompted the development of novel therapies.RO-101® is an antimicrobial gel that delivers therapeutic levels of hydrogen peroxide(H2O2),a reactive oxygen species,directly to the wound bed.In this study,electrospinning was used to incorporate RO-101® into a polyvinyl alcohol(PVA)sub-micron fibrous mesh that can act as a delivery agent,achieve a sustained release profile,and provide a barrier against infection.Adequate incorporation of this gel into sub-micron fibres was confirmed via nuclear magnetic resonance spectroscopy.Furthermore,scanning electron microscopy exhibited smooth and uniform meshes with diameters in the 200-500 nm range.PVA/RO-101 electrospun meshes generated H2O2 in concentrations exceeding 1 mM/(g·mL)(1 mM=1 mmol/L)after 24 h,and the role of sterilisation on H2O2 release was evaluated.PVA/RO-101 meshes exhibited antimicrobial activity against both Gram-positive Staphylococcus aureus(S.aureus)and Gram-negative Pseudomonas aeruginosa(P.aeruginosa)bacteria,achieving viable count reductions of up to 1 log unit CFU/mm2(CFU:colony-forming units).Moreover,these meshes were capable of disrupting biofilm formation,even against multidrug-resistant organisms such as methicillin-resistant S.aureus(MRSA).Furthermore,increasing the RO-101® concentration resulted in higher H2O2 production and an enhanced antimicrobial effect,while fibroblast cell viability and proliferation tests showed a concentration-dependent response with high cytocompatibility at low RO-101® concentrations.This study therefore demon-strates the potential of highly absorbent PVA/RO-101 meshes as potential antimicrobial wound dressings.

外文关键词：

AntimicrobialElectrospinningHydrogen peroxidePolyvinyl alcoholReactive oxygen speciesTissue engineeringWound dressing

作者：

Joel Yupanqui Mieles、Cian Vyas、Evangelos Daskalakis、Mohamed Hassan、James Birkett、Abdalla M.Omar、Gavin Humphreys、Carl Diver、Paulo Bartolo

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作者单位：

Department of Mechanical,Aerospace and Civil Engineering,University of Manchester,Manchester M13 9PL,UK

Singapore Centre for 3D Printing,School of Mechanical and Aerospace Engineering,Nanyang Technological University,Singapore 639798,Singapore

Department of Chemistry,University of Manchester,Manchester M13 9PL,UK

Division of Pharmacy and Optometry,University of Manchester,Manchester M13 9PL,UK

Department of Engineering,Manchester Metropolitan University,Manchester M13 9PL,UK

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出版年：

2024

DOI：

10.1007/s42242-024-00312-3

生物设计与制造(英文)

ISSN：2096-5524

年,卷(期)：2024.7(6)