查看更多>>摘要：AIM:To study the polymorphisms of toll-like receptor 4 (TLR4) gene Asp299Gly,Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp and susceptibility to inflammatory bowel disease (IBD) in the Zhuang population from Guangxi,China.METHODS:A case-control study was performed from February 2007 to October 2011 which included 146 Zhuang patients with IBD in the experimental group and 164 healthy Zhuang subjects who acted as the control group.All patients and healthy subjects were from the Guangxi Zhuang Autonomous Region of China.Genomic DNA was extracted from intestinal tissue by the phenol chloroform method.TLR4 gene Asp299Gly,Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp were amplified by polymerase chain reaction (PCR),and then detected by PCR-restriction fragment length polymorphism (RFLP).RESULTS:The TLR4 gene Asp299Gly was digested using Nco I restriction enzyme,and a single band of 249 bp was observed which showed that it was a wild type (AA).The TLR4 gene Thr399Ile was digested using Hinf Ⅰ restriction enzyme and only the wild type (CC) was detected.In addition,the TLR2 gene Arg677Trp was digested using Aci Ⅰ restriction enzyme and only the wild type (CC) was detected.The TLR2 gene Arg753Gln was digested using Pst Ⅰ restriction enzyme.Only the wild type (GG) as a single band of 254 bp was observed during RFLP.Overall,no heterozygous or homozygous single nucleotide polymorphism mutations were found in patients with Crohn's disease and ulcerative colitis both in the TLR4 geneAsp299Gly,Thr399Ile and the TLR2 gene Arg677Trp,Arg753Gln in the Zhuang population from the Guangxi Zhuang Autonomous Region of China.CONCLUSION:The TLR4 gene Asp299Gly,Thr399Ile and TLR2 gene Arg753Gln,Arg677Trp polymorphisms may not be associated with IBD in the Zhuang population from the Guangxi Zhuang Autonomous Region of China.
查看更多>>摘要：Hepatocellular carcinoma (HCC) is difficult to eradicate due to its resilient nature.Portal vein is often involved in tumors of large size,which exclude the patient from surgical resection and local ablative therapy,such as percutaneous ethanol injection (PEI) and radiofrequency ablation (RFA) because they were considered neither effective nor safe.Currently,there is almost no effective treatment for HCC of such condition.As a unique antitumor agent in form of lipophilic fluid for local injection,para-toluenesulfonamide (PTS) produces mild side effects while necrotizing the tumor tissues quickly and efficiently.Being largely different from both PEI and RFA therapies,PTS can disseminate itself in tumors more easily than other caustic agents,such as alcohol.So PTS may offer additional benefit to HCCs with vascular involvement.We herein describe a 70-year-old HCC patient who was treated with the combination of PTS injection and transcatheter arterial chemoembolization,resulting in a significantly improved clinical prognosis.
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