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畜牧与生物技术杂志(英文版)
中国科学技术协会
畜牧与生物技术杂志(英文版)

中国科学技术协会

李德发

季刊

1674-9782

zgxm@cau.edu.cn;xmzz@cau.edu.cn

010-62734403/2723

100193

中国农业大学西校区动物科技学院Journal of Animal Science and Biotechnology编辑部

畜牧与生物技术杂志(英文版)/Journal Journal of Animal Science and BiotechnologyCSCDCSTPCD北大核心SCI
查看更多>>定位为国内外公开发行的专业学术期刊，宗旨是立足国内，面向国际，为国内外畜牧科技工作者提供国际化学术共享的园地，促进我国与国际上其他国家在畜牧科学领域的交流；让中国畜牧科技走向世界，让世界了解中国畜牧科技。
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    Dietary fat supplementation relieves cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis in pigs

    Wei HeXinyu LiuYe FengHongwei Ding...
    1669-1687页
    查看更多>>摘要：Background Cold stress has negative effects on the growth and health of mammals,and has become a factor restricting livestock development at high latitudes and on plateaus.The gut-liver axis is central to energy metabolism,and the mechanisms by which it regulates host energy metabolism at cold temperatures have rarely been illustrated.In this study,we evaluated the status of glycolipid metabolism and oxidative stress in pigs based on the gut-liver axis and propose that AMP-activated protein kinase(AMPK)is a key target for alleviating energy stress at cold tempera-tures by dietary fat supplementation.Results Dietary fat supplementation alleviated the negative effects of cold temperatures on growth performance and digestive enzymes,while hormonal homeostasis was also restored.Moreover,cold temperature exposure increased glucose transport in the jejunum.In contrast,we observed abnormalities in lipid metabolism,which was characterized by the accumulation of bile acids in the ileum and plasma.In addition,the results of the ileal metabolomic analysis were consistent with the energy metabolism measurements in the jejunum,and dietary fat supplementation increased the activity of the mitochondrial respiratory chain and lipid metabolism.As the central nexus of energy metabolism,the state of glycolipid metabolism and oxidative stress in the liver are inconsistent with that in the small intestine.Specifically,we found that cold temperature exposure increased glucose transport in the liver,which fully validates the idea that hormones can act on the liver to regulate glucose output.Addition-ally,dietary fat supplementation inhibited glucose transport and glycolysis,but increased gluconeogenesis,bile acid cycling,and lipid metabolism.Sustained activation of AMPK,which an energy receptor and regulator,leads to oxidative stress and apoptosis in the liver;dietary fat supplementation alleviates energy stress by reducing AMPK phosphorylation.Conclusions Cold stress reduced the growth performance and aggravated glycolipid metabolism disorders and oxidative stress damage in pigs.Dietary fat supplementation improved growth performance and alleviated cold temperature-induced energy stress through AMPK-mediated mitochondrial homeostasis.In this study,we high-light the importance of AMPK in dietary fat supplementation-mediated alleviation of host energy stress in response to environmental changes.
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    Probiotic Pediococcus pentosaceus restored gossypol-induced intestinal barrier injury by increasing propionate content in Nile tilapia

    Feifei DingNannan ZhouYuan LuoTong Wang...
    1688-1703页
    查看更多>>摘要：Background Intestinal barrier is a dynamic interface between the body and the ingested food components,how-ever,dietary components or xenobiotics could compromise intestinal integrity,causing health risks to the host.Gossypol,a toxic component in cottonseed meal(CSM),caused intestinal injury in fish or other monogastric animals.It has been demonstrated that probiotics administration benefits the intestinal barrier integrity,but the efficacy of probiotics in maintaining intestinal health when the host is exposed to gossypol remains unclear.Here,a strain(YC)affiliated to Pediococcus pentosaceus was isolated from the gut of Nile tilapia(Oreochromis niloticus)and its potential to repair gossypol-induced intestinal damage was evaluated.Results A total of 270 Nile tilapia(2.20±0.02 g)were allotted in 3 groups with 3 tanks each and fed with 3 diets including CON(control diet),GOS(control diet containing 300mg/kg gossypol)and GP(control diet containing 300mg/kg gossypol and 108colony-forming unit(CFU)/g P.pentosaceus YC),respectively.After 10weeks,addition of P.pentosaceus YC restored growth retardation and intestinal injury induced by gossypol in Nile tilapia.Transcriptome analysis and siRNA interference experiments demonstrated that NOD-like receptors(NLR)family caspase recruitment domain(CARD)domain containing 3(Nlrc3)inhibition might promote intestinal stem cell(ISC)proliferation,as well as maintaining gut barrier integrity.16S rRNA sequencing and gas chromatography-mass spectrometry(GC-MS)revealed that addition of P.pentosaceus YC altered the composition of gut microbiota and increased the content of propionate in fish gut.In vitro studies on propionate's function demonstrated that it suppressed nlrc3 expression and promoted wound healing in Caco-2 cell model.Conclusions The present study reveals that P.pentosaceus YC has the capacity to ameliorate intestinal barrier injury by modulating gut microbiota composition and elevating propionate level.This finding offers a promising strategy for the feed industry to incorporate cottonseed meal into fish feed formulations.
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    Dietary sodium acetate and sodium butyrate improve high-carbohydrate diet utilization by regulating gut microbiota,liver lipid metabolism,oxidative stress,and inflammation in largemouth bass(Micropterus salmoides)

    Qiao LiuLiangshun ChengMaozhu WangLianfeng Shen...
    1704-1722页
    查看更多>>摘要：Background Adequate level of carbohydrates in aquafeeds help to conserve protein and reduce cost.However,studies have indicated that high-carbohydrate(HC)diet disrupt the homeostasis of the gut-liver axis in largemouth bass,resulting in decreased intestinal acetate and butyrate level.Method Herein,we had concepted a set of feeding experiment to assess the effects of dietary sodium acetate(SA)and sodium butyrate(SB)on liver health and the intestinal microbiota in largemouth bass fed an HC diet.The experi-mental design comprised 5 isonitrogenous and isolipidic diets,including LC(9％starch),HC(18％starch),HCSA(18％starch;2 g/kg SA),HCSB(18％starch;2 g/kg SB),and HCSASB(18％starch;1 g/kg SA+1 g/kg SB).Juvenile largemouth bass with an initial body weight of 7.00±0.20 g were fed on these diets for 56 d.Results We found that dietary SA and SB reduced hepatic triglyceride accumulation by activating autophagy(ATG101,LC3B and TFEB),promoting lipolysis(CPT1α,HSL and AMPKα),and inhibiting adipogenesis(FAS,ACCA,SCD1 and PPARγ).In addition,SA and SB decreased oxidative stress in the liver(CAT GPX1α and SOD1)by activating the Keap1-Nrf2 pathway.Meanwhile,SA and SB alleviated HC-induced inflammation by downregulating the expres-sion of pro-inflammatory factors(IL-1β,COX2 and Hepcidin1)through the NF-κB pathway.Importantly,SA and SB increased the abundance of bacteria that produced acetic acid and butyrate(Clostridium_sensu_stricto_1).Combined with the KEGG analysis,the results showed that SA and SB enriched carbohydrate metabolism and amino acid metab-olism pathways,thereby improving the utilization of carbohydrates.Pearson correlation analysis indicated that growth performance was closely related to hepatic lipid deposition,autophagy,antioxidant capacity,inflammation,and intes-tinal microbial composition.Conclusions In conclusion,dietary SA and SB can reduce hepatic lipid deposition;and alleviate oxidative stress and inflammation in largemouth bass fed on HC diet.These beneficial effects may be due to the altered composi-tion of the gut microbiota caused by SA and SB.The improvement effects of SB were stronger than those associated with SA.
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    Dietary eubiotics of microbial muramidase and glycan improve intestinal villi,ileum microbiota composition and production trait of broiler

    Sungbo ChoShanmugam Suresh KumarSantiago RamirezRolando Valientes...
    1723-1736页
    查看更多>>摘要：Background Optimal gut health is important to maximize growth performance and feed efficiency in broiler chick-ens.A total of 1,365 one-day-old male Ross 308 broiler chickens were randomly divided into 5 treatments groups with 21 replicates,13 birds per replicate.The present research investigated effects of microbial muramidase or a preci-sion glycan alone or in combination on growth performance,apparent total tract digestibility,total blood carotenoid content,intestinal villus length,meat quality and gut microbiota in broiler chickens.Treatments included:NC:nega-tive control(basal diet group);PC:positive control(basal diet+0.02％probiotics);MR:basal diet+0.035％microbial muramidase;PG:basal diet+0.1％precision glycan;and MRPG:basal diet+0.025％MR+0.1％PG,respectively.Results MRPG group increased the body weight gain and feed intake(P＜0.05)compared with NC group.Moreover,it significantly increased total serum carotenoid(P＜0.05)and MRPG altered the microbial diversity in ileum contents.The MRPG treatment group increased the abundance of the phylum Firmicutes,and family Lachnospiraceae,Rumino-coccaceae,Oscillospiraceae,Lactobacillaceae,Peptostreptococcaceae and decreased the abundance of the phylum Campilobacterota,Bacteroidota and family Bacteroidaceae.Compared with the NC group,the chickens fed MRPG showed significantly increased in duodenum villus length at end the trial.Conclusion In this study,overall results showed that the synergetic effects of MR and PG showed enhancing growth performance,total serum carotenoid level and altering gut microbiota composition of broilers.The current research indicates that co-supplementation of MR and PG in broiler diets enhances intestinal health,consequently leading to an increased broiler production.
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    Hesperidin ameliorates H2O2-induced bovine mammary epithelial cell oxidative stress via the Nrf2 signaling pathway

    Qi HuangJiashuo LiuCan PengXuefeng Han...
    1737-1750页
    查看更多>>摘要：Background Hesperidin is a citrus flavonoid with anti-inflammatory and antioxidant potential.However,its protec-tive effects on bovine mammary epithelial cells(bMECs)exposed to oxidative stress have not been elucidated.Results In this study,we investigated the effects of hesperidin on H2O2-induced oxidative stress in bMECs and the underlying molecular mechanism.We found that hesperidin attenuated H2O2-induced cell damage by reduc-ing reactive oxygen species(ROS)and malondialdehyde(MDA)levels,increasing catalase(CAT)activity,and improv-ing cell proliferation and mitochondrial membrane potential.Moreover,hesperidin activated the Keap1/Nrf2/ARE signaling pathway by inducing the nuclear translocation of Nrf2 and the expression of its downstream genes NQO1 and HO-1,which are antioxidant enzymes involved in ROS scavenging and cellular redox balance.The protective effects of hesperidin were blocked by the Nrf2 inhibitor ML385,indicating that they were Nrf2 dependent.Conclusions Our results suggest that hesperidin could protect bMECs from oxidative stress injury by activating the Nrf2 signaling pathway,suggesting that hesperidin as a natural antioxidant has positive potential as a feed addi-tive or plant drug to promote the health benefits of bovine mammary.
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    Pelleting and particle size reduction of corn increase net energy and digestibility of fiber,protein,and fat in corn-soybean meal diets fed to group-housed pigs

    Su A LeeDiego A.RodriguezChad B.PaulkHans H.Stein...
    1751-1760页
    查看更多>>摘要：Background Reduction of the particle size of corn increases energy digestibility and concentrations of digestible and metabolizable energy.Pelleting may also reduce particle size of grain,but it is not known if there are interactions between particle size reduction and pelleting.The objective of this experiment was to test the hypothesis that par-ticle size reduction and pelleting,separately or in combination,increase N balance,apparent total tract digestibility(ATTD)of fiber and fat,and net energy(NE)in corn-soybean meal diets fed to group-housed pigs.Methods Six corn-soybean meal-based diets were used in a 3 × 2 factorial design with 3 particle sizes of corn(i.e.,700,500,or 300 μm)and 2 diet forms(i.e.,meal or pelleted).Pigs were allowed ad libitum access to feed and water.Twenty-four castrated male pigs(initial weight:29.52 kg;standard diviation:1.40)were allotted to the 6 diets using a 6 × 6 Latin square design with 6 calorimeter chambers(i.e.,4 pigs/chamber)and 6 periods.Oxygen consump-tion and CO2 and CH4 productions were measured during fed and fasting states and fecal and urine samples were collected.Results Regardless of particle size of corn,the ATTD of gross energy(GE),N,and acid-hydrolyzed ether extract(AEE),and the concentration of NE were greater(P＜0.05)in pelleted diets than in meal diets.Regardless of diet form,the ATTD of GE,N,and AEE,and the concentration of NE were increased(linear;P＜0.05)by reducing the particle size of corn,but the increase was greater in meal diets than in pelleted diets(interaction;P＜0.05).Conclusions Both pelleting and reduction of corn particle size increased nutrient digestibility and NE,but increases were greater in meal diets than in pelleted diets.
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