首页|Hesperidin ameliorates H2O2-induced bovine mammary epithelial cell oxidative stress via the Nrf2 signaling pathway
Hesperidin ameliorates H2O2-induced bovine mammary epithelial cell oxidative stress via the Nrf2 signaling pathway
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Background Hesperidin is a citrus flavonoid with anti-inflammatory and antioxidant potential.However,its protec-tive effects on bovine mammary epithelial cells(bMECs)exposed to oxidative stress have not been elucidated.Results In this study,we investigated the effects of hesperidin on H2O2-induced oxidative stress in bMECs and the underlying molecular mechanism.We found that hesperidin attenuated H2O2-induced cell damage by reduc-ing reactive oxygen species(ROS)and malondialdehyde(MDA)levels,increasing catalase(CAT)activity,and improv-ing cell proliferation and mitochondrial membrane potential.Moreover,hesperidin activated the Keap1/Nrf2/ARE signaling pathway by inducing the nuclear translocation of Nrf2 and the expression of its downstream genes NQO1 and HO-1,which are antioxidant enzymes involved in ROS scavenging and cellular redox balance.The protective effects of hesperidin were blocked by the Nrf2 inhibitor ML385,indicating that they were Nrf2 dependent.Conclusions Our results suggest that hesperidin could protect bMECs from oxidative stress injury by activating the Nrf2 signaling pathway,suggesting that hesperidin as a natural antioxidant has positive potential as a feed addi-tive or plant drug to promote the health benefits of bovine mammary.
Qi Huang、Jiashuo Liu、Can Peng、Xuefeng Han、Zhiliang Tan
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CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Hunan Provincial Key Laboratory of Ani-mal Nutritional Physiology and Metabolic Process,Institute of Subtropical Agri-culture,Chinese Academy of Sciences,Changsha 410125,Hunan,China
State Key Laboratory of Animal Nutrition and Feeding,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China
Strategic Priority Research Program of the Chinese Academy of Sciences
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