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园艺研究（英文）
园艺研究（英文）
园艺研究（英文）/Journal Horticulture ResearchCSCDCSTPCDSCI
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    CsMYB67 participates in the flavonoid biosynthesis of summer tea leaves

    Ying YeRu-Yi LiuXin LiXin-Qiang Zheng...
    1-12页
    查看更多>>摘要：Flavonoids are important compounds in tea leaves imparting bitter and astringent taste,which also play key roles in tea plants responding to environmental stress.Our previous study showed that the expression level of CsMYB67 was positively correlated with the accumulation of flavonoids in tea leaves as exposed to sunlight.Here,we newly reported the function of CsMYB67 in regulating flavonoid biosynthesis in tea leaves.CsMYB67 was localized in the nucleus and responded to temperature.The results of transient expression assays showed the co-transformation of CsMYB67 and CsTTG1 promoted the transcription of CsANS promoter in the tobacco system.CsTTG1 was bound to the promoter of CsANS based on the results of yeast one-hybrid(Y1H)and transient expression assays,while CsMYB67 enhanced the transcription of CsANS through protein interaction with CsTTG1 according to the results of yeast two-hybrid(Y2H)and bimolecular fluorescence complementation(BiFC).Thus,CsMYB67-CsTTG1 module enhanced the anthocyanin biosynthesis through up-regulating the transcription of CsANS.Besides,CsMYB67 also enhanced the transcription of CsFLS and CsUFGT through forming transcription factor complexes.The function of CsMYB67 on flavonoid biosynthesis in tea leaves was validated by gene suppression assay.As CsMYB67 was suppressed,the transcriptional level of CsFLS was greatly reduced,leading to a significant increase in the contents of total catechins and total anthocyanidins.Hence,CsMYB67 plays an important role in regulating the downstream pathway of flavonoid biosynthesis in summer tea leaves.
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    Development of virus-induced genome editing methods in Solanaceous crops

    Seo-Young LeeBomi KangJelli VenkateshJoung-Ho Lee...
    13-26页
    查看更多>>摘要：Genome editing(GE)using CRISPR/Cas systems has revolutionized plant mutagenesis.However,conventional transgene-mediated GE methods have limitations due to the time-consuming generation of stable transgenic lines expressing the Cas9/single guide RNA(sgRNA)module through tissue cultures.Virus-induced genome editing(VIGE)systems have been successfully employed in model plants,such as Arabidopsis thaliana and Nicotiana spp.In this study,we developed two VIGE methods for Solanaceous plants.First,we used the tobacco rattle virus(TRV)vector to deliver sgRNAs into a transgenic tomato(Solanum lycopersicum)line of cultivar Micro-Tom expressing Cas9.Second,we devised a transgene-free GE method based on a potato virus X(PVX)vector to deliver Cas9 and sgRNAs.We designed and cloned sgRNAs targeting Phytoene desaturase in the VIGE vectors and determined optimal conditions for VIGE.We evaluated VIGE efficiency through deep sequencing of the target gene after viral vector inoculation,detecting 40.3％and 36.5％mutation rates for TRV-and PVX-mediated GE,respectively.To improve editing efficiency,we applied a 37℃ heat treatment,which increased the editing efficiency by 33％to 46％and 56％to 76％for TRV-and PVX-mediated VIGE,respectively.To obtain edited plants,we subjected inoculated cotyledons to tissue culture,yielding successful editing events.We also demonstrated that PVX-mediated GE can be applied to other Solanaceous crops,such as potato(Solanum tuberosum)and eggplant(Solanum melongena).These simple and highly efficient VIGE methods have great potential for generating genome-edited plants in Solanaceous crops.
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    Genetic architecture and genomic prediction of plant height-related traits in chrysanthemum

    Xuefeng ZhangJiangshuo SuFeifei JiaYuhua He...
    27-38页
    查看更多>>摘要：Plant height(PH)is a crucial trait determining plant architecture in chrysanthemum.To better understand the genetic basis of PH,we investigated the variations of PH,internode number(IN),internode length(IL),and stem diameter(SD)in a panel of 200 cut chrysanthemum accessions.Based on 330 710 high-quality SNPs generated by genotyping by sequencing,a total of 42 associations were identified via a genome-wide association study(GWAS),and 16 genomic regions covering 2.57 Mb of the whole genome were detected through selective sweep analysis.In addition,two SNPs,Chr1_339370594 and Chr18_230810045,respectively associated with PH and SD,overlapped with the selective sweep regions from FST and n ratios.Moreover,candidate genes involved in hormones,growth,transcriptional regulation,and metabolic processes were highlighted based on the annotation of homologous genes in Arabidopsis and transcriptomes in chrysanthemum.Finally,genomic selection for four PH-related traits was performed using a ridge regression best linear unbiased predictor model(rrBLUP)and six marker sets.The marker set constituting the top 1000 most significant SNPs identified via GWAS showed higher predictabilities for the four PH-related traits,ranging from 0.94 to 0.97.These findings improve our knowledge of the genetic basis of PH and provide valuable markers that could be applied in chrysanthemum genomic selection breeding programs.
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    Integrated proteomic analysis reveals interactions between phosphorylation and ubiquitination in rose response to Botrytis infection

    Rui LiJuanni YaoYue MingJia Guo...
    39-53页
    查看更多>>摘要：As two of the most abundant post-translational modifications,phosphorylation and ubiquitination play a significant role in modulating plant-pathogen interactions and increasing evidence indicates their crosstalk in plant immunity.Rose(Rosa sp.)is one of the most important ornamental plants and can be seriously infected by Botrytis cinerea.Here,integrated proteomics analysis was performed to detect global proteome,phosphorylation,and ubiquitination changes in rose upon B.cinerea infection and investigate the possible phosphorylation and ubiquitination crosstalk.A total of 6165 proteins,11774 phosphorylation and 10 582 ubiquitination sites,and 77 phosphorylation and 13 ubiquitination motifs were identified.Botrytis cinerea infection resulted in 169 up-regulated and 122 down-regulated proteins,291 up-regulated and 404 down-regulated phosphorylation sites,and 250 up-regulated and 634 down-regulated ubiquitination sites.There were 12 up-regulated PR10 proteins and half of them also showed reduced ubiquitination.A lot of kinases probably involved in plant pattern-triggered immunity signaling were up-regulated phosphoproteins.Noticeably,numerous kinases and ubiquitination-related proteins also showed a significant change in ubiquitination and phosphorylation,respectively.A cross-comparison of phosphoproteome and ubiquitylome indicated that both of two post-translational modifications of 104 proteins were dynamically regulated,and many putative pattern-triggered immunity signaling components in the plant plasma membrane were co-regulated.Moreover,five selected proteins,including four PR10 proteins and a plasma membrane aquaporin,were proven to be involved in rose resistance to B.cinerea.Our study provides insights into the molecular mechanisms underlying rose resistance to B.cinerea and also increases the database of phosphorylation and ubiquitination sites in plants.
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    Two transcription factors,AcREM14 and AcC3H1,enhance the resistance of kiwifruit Actinidia chinensis var.chinensis to Pseudomonas syringae pv.actinidiae

    Chao ZhaoWei LiuYali ZhangYuanzhe Li...
    55-70页
    查看更多>>摘要：Kiwifruit bacterial canker is a global disease caused by Pseudomonas syringae pv.actinidiae(Psa),which poses a major threat to kiwifruit production worldwide.Despite the economic importance of Actinidia chinensis var.chinensis,only a few resistant varieties have been identified to date.In this study,we screened 44 kiwifruit F1 hybrid lines derived from a cross between two A.chinensis var.chinensis lines and identified two offspring with distinct resistance to Psa:resistant offspring RH12 and susceptible offspring SH14.To identify traits associated with resistance,we performed a comparative transcriptomic analysis of these two lines.We identified several highly differentially expressed genes(DEGs)associated with flavonoid synthesis,pathogen interactions,and hormone signaling pathways,which play essential roles in disease resistance.Additionally,using weighted gene co-expression network analysis,we identified six core transcription factors.Moreover,qRT-PCR results demonstrated the high expression of AcC3H1 and AcREM14 in Psa-induced highly resistant hybrid lines.Ultimately,Overexpression of AcC3H1 and AcREM14 in kiwifruit enhanced disease resistance,and this was associated with upregulation of enzymatic activity and gene expression in the salicylic acid(SA)signaling pathway.Our study elucidates a molecular mechanism underlying disease resistance in kiwifruit and contributes to the advancement of research on kiwifruit breeding.
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    Genome-wide association analysis identifies a candidate gene controlling seed size and yield in Xanthoceras sorbifolium Bunge

    Ziquan ZhaoChongjun LiangWei ZhangYingying Yang...
    71-83页
    查看更多>>摘要：Yellow horn(Xanthoceras sorbifolium Bunge)is a woody oilseed tree species whose seed oil is rich in unsaturated fatty acids and rare neuronic acids,and can be used as a high-grade edible oil or as a feedstock for biodiesel production.However,the genetic mechanisms related to seed yield in yellow horn are not well elucidated.This study identified 2164 863 SNP loci based on 222 genome-wide resequencing data of yellow horn germplasm.We conducted genome-wide association study(GWAS)analysis on three core traits(hundred-grain weight,single-fruit seed mass,and single-fruit seed number)that influence seed yield for the years 2022 and 2020,and identified 399 significant SNP loci.Among these loci,the Chr10_24013014 and Chr10_24012613 loci caught our attention due to their consistent associations across multiple analyses.Through Sanger sequencing,we validated the genotypes of these two loci across 16 germplasms,confirming their consistency with the GWAS analysis results.Downstream of these two significant loci,we identified a candidate gene encoding an AP2 transcription factor protein,which we named XsAP2.RT-qPCR analysis revealed high expression of the XsAP2 gene in seeds,and a significant negative correlation between its expression levels and seed hundred-grain weight,as well as single-fruit seed mass,suggesting its potential role in the normal seed development process.Transgenic Arabidopsis lines with the overexpressed XsAP2 gene exhibited varying degrees of reduction in seed size,number of seeds per silique,and number of siliques per plant compared with wild-type Arabidopsis.Combining these results,we hypothesize that the XsAP2 gene may have a negative regulatory effect on seed yield of yellow horn.These results provide a reference for the molecular breeding of high-yielding yellow horn.
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    The haplotype-resolved telomere-to-telomere carnation(Dianthus caryophyllus)genome reveals the correlation between genome architecture and gene expression

    Lan LanLuhong LengWeichao LiuYonglin Ren...
    85-97页
    查看更多>>摘要：Carnation(Dianthus caryophyllus)is one of the most valuable commercial flowers,due to its richness of color and form,and its excellent storage and vase life.The diverse demands of the market require faster breeding in carnations.A full understanding of carnations is therefore required to guide the direction of breeding.Hence,we assembled the haplotype-resolved gap-free carnation genome of the variety'Baltico',which is the most common white standard variety worldwide.Based on high-depth HiFi,ultra-long nanopore,and Hi-C sequencing data,we assembled the telomere-to-telomere(T2T)genome to be 564479117 and 568 266 215 bp for the two haplotypes Hap1 and Hap2,respectively.This T2T genome exhibited great improvement in genome assembly and annotation results compared with the former version.The improvements were seen when different approaches to evaluation were used.Our T2T genome first informs the analysis of the telomere and centromere region,enabling us to speculate about specific centromere characteristics that cannot be identified by high-order repeats in carnations.We analyzed allele-specific expression in three tissues and the relationship between genome architecture and gene expression in the haplotypes.This demonstrated that the length of the genes,coding sequences,and introns,the exon numbers and the transposable element insertions correlate with gene expression ratios and levels.The insertions of transposable elements repress expression in gene regulatory networks in carnation.This gap-free finished T2T carnation genome provides a valuable resource to illustrate the genome characteristics and for functional genomics analysis in further studies and molecular breeding.
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    MODMS:a multi-omics database for facilitating biological studies on alfalfa(Medicago sativa L.)

    Longfa FangTao LiuMingyu LiXueMing Dong...
    99-108页
    查看更多>>摘要：Alfalfa(Medicago sativa L.)is a globally important forage crop.It also serves as a vegetable and medicinal herb because of its excellent nutritional quality and significant economic value.Multi-omics data on alfalfa continue to accumulate owing to recent advances in high-throughput techniques,and integrating this information holds great potential for expediting genetic research and facilitating advances in alfalfa agronomic traits.Therefore,we developed a comprehensive database named MODMS(multi-omics database of M.sativa)that incorporates multiple reference genomes,annotations,comparative genomics,transcriptomes,high-quality genomic variants,proteomics,and metabolomics.This report describes our continuously evolving database,which provides researchers with several convenient tools and extensive omics data resources,facilitating the expansion of alfalfa research.Further details regarding the MODMS database are available at https://modms.lzu.edu.cn/.
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    A putative E3 ubiquitin ligase substrate receptor degrades transcription factor SmNAC to enhance bacterial wilt resistance in eggplant

    Shuangshuang YanYixi WangBingwei YuYuwei Gan...
    109-121页
    查看更多>>摘要：Bacterial wilt caused by Ralstonia solanacearum is a severe soil-borne disease globally,limiting the production in Solanaceae plants.SmNAC negatively regulated eggplant resistance to Bacterial wilt(BW)though restraining salicylic acid(SA)biosynthesis.However,other mechanisms through which SmNAC regulates BW resistance remain unknown.Here,we identified an interaction factor,SmDDA1b,encoding a substrate receptor for E3 ubiquitin ligase,from the eggplant cDNA library using SmNAC as bait.SmDDA1b expression was promoted by R.solanacearum inoculation and exogenous SA treatment.The virus-induced gene silencing of the SmDDA1b suppressed the BW resistance of eggplants;SmDDA1b overexpression enhanced the BW resistance of tomato plants.SmDDA1b positively regulates BW resistance by inhibiting the spread of R.solanacearum within plants.The SA content and the SA biosynthesis gene ICS1 and signaling pathway genes decreased in the SmDDA1b-silenced plants but increased in SmDDA1b-overexpression plants.Moreover,SmDDB1 protein showed interaction with SmCUL4 and SmDDA1b and protein degradation experiments indicated that SmDDA1b reduced SmNAC protein levels through proteasome degradation.Furthermore,SmNAC could directly bind the SmDDA1b promoter and repress its transcription.Thus,SmDDA1b is a novel regulator functioning in BW resistance of solanaceous crops via the SmNAC-mediated SA pathway.Those results also revealed a negative feedback loop between SmDDA1b and SmNAC controlling BW resistance.
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    Construction of a high-density genetic map for yardlong bean and identification of ANT1 as a regulator of anthocyanin biosynthesis

    Hongmei ZhangWei ZhangShan MengLinchong Hui...
    123-132页
    查看更多>>摘要：Because its long,tender pods supply essential proteins,vitamins,and fibers to humans,yardlong bean(Vigna unguiculata ssp.sesquipedalis)is a commonly consumed vegetable,especially in Southeast Asia.To provide insights into the genetic bases of key agricultural traits in yardlong bean,we here created a high-density bin-map with 2084 bin markers using 514 227 SNPs from a recombinant-inbred line(RIL)population.Quantitative trait loci(QTL)mapping was carried out to identify loci associated with anthocyanin content(ANT),vitamin E content(VE),total soluble protein content(TSP),pod length(PL),hundred-seed weight(HSW),seed length and width(SL and SW,respectively),and seed coat color(SCC).In total,20 related QTLs were isolated,explaining 7.58-56.03％of the phenotypic variation.Of these,five major QTLs(qANT5,qTSP11,qVE7,qPL3,and qSCC9)were detected in 2020,2021,and the combined environment,explaining 11.96-56.03％of the phenotypic variation.VuANT1 was identified as a causal gene for the QTL qANT5,which regulated anthocyanin content;VuANT1 was highly expressed in immature purple pods but barely detectable in white pods.VuANT1 overexpression in tobacco leaves and yardlong bean hairy roots resulted in purple coloration as a result of anthocyanin accumulation.These findings suggested that VuANT1 was a key regulator of anthocyanin accumulation in yardlong bean.Our results lay a firm foundation for target agricultural trait improvement and clarification of the genetic mechanisms underlying agricultural traits in yardlong bean.
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