查看更多>>摘要：Magnesium(Mg2+)is a crucial nutrient for the growth and development of Camellia sinensis and is closely related to the quality of tea.However,the underlying mechanisms responding to low-Mg 2+stress in tea plants remain largely unknown.In this study,photosynthetic parameters,metabolomics,and transcriptomics were utilized to explore the potential effects of low Mg2+on the growth and metabolism of C.sinensis.Low-Mg2+treatment increased the ratio of shoot dry weight to root dry weight but decreased the photosynthesis of C.sinensis.Forty and thirty metabolites were impacted by Mg2+shortage in C.sinensis shoots and roots,respectively.Integrated transcriptome and metabolome analyses revealed the possible reasons for the decreased contents of chlorophyll and catechins and the increased theanine content in C.sinensis roots.Weighted gene co-expression network analysis indicated that the Mg2+transport system was essential in the regulation of Mg2+homeostasis in C.sinensis,in which CsMGT5 was identified to be the key regulator according to CsMGT5-overexpressing and complementary assays in Arabidopsis thaliana.Moreover,silencing of CsMGT5 in vivo reduced the content of chlorophyll in C.sinensis shoots.In addition,CsMGT5 might collaborate with ammonium transporters to keep the amino acid content steady,suggesting its potential application for tea quality improvement.All these findings demonstrate the key roles of CsMGTs for Mg2+homeostasis in C.sinensis,providing a theoretical basis for Mg2+efficient utilization in plants.
查看更多>>摘要：Mango(Mangifera indica L.)has been widely cultivated as a culturally and economically significant fruit tree for roughly 4000 years.Despite its rich history,little is known about the crop's domestication,genomic variation,and the genetic loci underlying agronomic traits.This study employs the whole-genome re-sequencing of 224 mango accessions sourced from 22 countries,with an average sequencing depth of 16.37 x,to explore their genomic variation and diversity.Through phylogenomic analysis,M.himalis J.Y.Liang,a species grown in China,was reclassified into the cultivated mango group known as M.indica.Moreover,our investigation of mango population structure and differentiation revealed that Chinese accessions could be divided into two distinct gene pools,indicating the presence of independent genetic diversity ecotypes.By coupling genome-wide association studies with analyses of genotype variation patterns and expression patterns,we identified several candidate loci and dominant genotypes associated with mango flowering capability,fruit weight,and volatile compound production.In conclusion,our study offers valuable insights into the genetic differentiation of mango populations,paving the way for future agronomic improvements through genomic-assisted breeding.
查看更多>>摘要：Stable genetic transformation of peach[Prunus persica(L.)Batsch]still faces many technical challenges,and existing transient expression methods are limited by tissue type or developmental stage,making it difficult to conduct functional analysis of genes regulating shoot growth.To overcome this dilemma,we developed a three-step method for efficient analysis of gene functions during peach seedling growth and development.This method resulted in transformation frequencies ranging from 48 to 87％,depending on the gene.From transformation of germinating seeds to phenotyping of young saplings took just 1.5 months and can be carried out any time of year.To test the applicability of this method,the function of three tree architecture-related genes,namely PpPDS,PpMAX4,and PpWEEP,and two lateral root-related genes,PpIAA14-1 and-2,were confirmed.Since functional redundancy can challenge gene functional analyses,tests were undertaken with the growth-repressor DELLA,which has three homologous genes,PpDGYLA(DG),PpDELLA1(D1),and-2(D2),in peach that are functionally redundant.Silencing using a triple-target vector(TRV2-DG-D1-D2)resulted in transgenic plants taller than those carrying just TRV2-DG or TRV2.Simultaneously silencing the three DELLA genes also attenuated the stature of two dwarf genotypes,'FHSXT'and'HSX',which normally accumulate DELLA proteins.Our study provides a method for the functional analysis of genes in peach and can be used for the study of root,stem,and leaf development.We believe this method can be replicated in other woody plants.
查看更多>>摘要：The grape genome is the basis for grape studies and breeding,and is also important for grape industries.In the last two decades,more than 44 grape genomes have been sequenced.Based on these genomes,researchers have made substantial progress in understanding the mechanism of biotic and abiotic resistance,berry quality formation,and breeding strategies.In addition,this work has provided essential data for future pangenome analyses.Apart from de novo assembled genomes,more than six whole-genome sequencing projects have provided datasets comprising almost 5000 accessions.Based on these datasets,researchers have explored the domestication and origins of the grape and clarified the gene flow that occurred during its dispersed history.Moreover,genome-wide association studies and other methods have been used to identify more than 900 genes related to resistance,quality,and developmental phases of grape.These findings have benefited grape studies and provide some basis for smart genomic selection breeding.Moreover,the grape genome has played a great role in grape studies and the grape industry,and the importance of genomics will increase sharply in the future.
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