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中国病毒学
中国病毒学

陈新文

双月刊

1674-0769

bjb@wh.iov.cn

027-87199157

430071

武汉武昌区小洪山中区44号

中国病毒学/Journal Virologica SinicaCSCD北大核心CSTPCDSCI
查看更多>>本刊由中国科学院武汉病毒研究所、中国微生物学会共同主办、科学出版社出版的学术性双月刊，创刊于1986年，原名为《病毒学杂志》(Virologica　Sinica)，季刊。1991年更名为《中国病毒学》，外文刊名不变，2003年改为双月刊，自创刊以来，发表病毒学研究论文1000多篇，发表论文基金率为65%以上。曾三次荣获湖北省优秀期刊奖，被评为中国生物学核心期刊、基础医学类核心期刊和中国科学引文数据库核心期刊。长期被BA（生物学文摘）、CA（化学文摘）和中国生物学文摘、医学文摘、农业学文摘等国内外20余种文摘及检索刊物收录，为国家科技部信息所“万方数据（ChinaInfo）系统”、清华大学“中国学术期刊光盘版”和“中国期刊网”的期刊源。是CSCI　(中国科学引文索引)、中国生物学和医学期刊的核心期刊。影响因子为0.553　(2003年统计数据)
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    Vagal-mAChR4 signaling promotes Friend virus complex(FV)-induced acute erythroleukemia

    Shuting SongZhekai LinCaiqi ZhaoJing Wen...
    429-439页
    查看更多>>摘要：Erythroleukemia belongs to acute myeloid leukemia(AML)type 6(M6),and treatment remains difficult due to the poor prognosis of the disease.Friend virus(FV)is a complex of two viruses:Friend murine leukemia virus(F-MuLV)strain along with a defective spleen focus-forming virus(SFFV),which can induce acute eryth-roleukemia in mice.We have previously reported that activation of vagal α7 nicotinic acetylcholine receptor(nAChR)signaling promotes HIV-1 transcription.Whether vagal muscarinic signaling mediates FV-induced erythroleukemia and the underlying mechanisms remain unclear.In this study,sham and vagotomized mice were intraperitoneally injected with FV.FV infection caused anemia in sham mice,and vagotomy reversed this change.FV infection increased erythroblasts ProE,EryA,and EryB cells in the spleen,and these changes were blocked by vagotomy.In bone marrow,FV infection reduced EryC cells in sham mice,an effect that was coun-teracted by vagotomy.FV infection increased choline acetyltransferase(ChAT)expression in splenic CD4+and CD8+T cells,and this change was reversed by vagotomy.Furthermore,the increase of EryA and EryB cells in spleen of FV-infected wild-type mice was reversed after deletion of ChAT in CD4+T cells.In bone marrow,FV infection reduced EryB and EryC cells in sham mice,whereas lack of ChAT in CD4+T cells did not affect this change.Activation of muscarinic acetylcholine receptor 4(mAChR4)by clozapine N-oxide(CNO)significantly increased EryB in the spleen but decreased the EryC cell population in the bone marrow of FV-infected mice.Thus,vagal-mAChR4 signaling in the spleen and bone marrow synergistically promotes the pathogenesis of acute erythroleukemia.We uncover an unrecognized mechanism of neuromodulation in erythroleukemia.
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    Temperature-regulated type Ⅱ grass carp reovirus establishes latent infection in Ctenopharyngodon idella brain

    Rui JiangJie ZhangZhiwei LiaoWentao Zhu...
    440-447页
    查看更多>>摘要：Grass carp reovirus(GCRV)causes extensive infection and death in grass carp and black carp fingerlings,with a highly seasonal prevalence.Previous studies suggested that GCRV can become latent after primary infection.In this study,we investigated type Ⅱ GCRV(GCRV-Ⅱ)latency in asymptomatic grass carp with GCRV infection or exposure history.We found that during latent infection,GCRV-Ⅱ was detectable only in the brain of grass carp,unlike the multi-tissue distribution observed in natural infection.GCRV-Ⅱ only caused damage to the brain during latent infection,while in natural infection,brain,heart,and eye tissues had relatively higher viral loads.We also discovered viral inclusion bodies in infected fish brains.Additionally,GCRV-Ⅱ distribution in grass carp was notably affected by ambient temperature,with the virus targeting the brain only during low temperatures and multi-tissue distribution during high temperatures.This study provides insights into the mechanisms of GCRV-Ⅱlatent infection and reactivation and contributes to the prevention and control of GCRV pandemics.
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    Spastin is required for human immunodeficiency virus-1 efficient replication through cooperation with the endosomal sorting complex required for transport(ESCRT)protein

    Wenyuan ShenChang LiuYue HuQian Ding...
    448-458页
    查看更多>>摘要：Human immunodeficiency virus-1(HIV-1)encodes simply 15 proteins and thus depends on multiple host cellular factors for virus reproduction.Spastin,a microtubule severing protein,is an identified HIV-1 dependency factor,but the mechanism regulating HIV-1 is unclear.Here,the study showed that knockdown of spastin inhibited the production of the intracellular HIV-1 Gag protein and new virions through enhancing Gag lysosomal degradation.Further investigation showed that increased sodium tolerance 1(IST1),the subunit of endosomal sorting complex required for transport(ESCRT),could interact with the MIT domain of spastin to regulate the intracellular Gag production.In summary,spastin is required for HIV-1 replication,while spastin-IST1 interaction facilitates virus production by regulating HIV-1 Gag intracellular trafficking and degradation.Spastin may serve as new target for HIV-1 prophylactic and therapy.
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    ASFV transcription reporter screening system identifies ailanthone as a broad antiviral compound

    Yuhang ZhangZhenjiang ZhangFan ZhangJiwen Zhang...
    459-469页
    查看更多>>摘要：African swine fever(ASF)is an acute,highly contagious and deadly viral disease in swine that jeopardizes the worldwide pig industry.Unfortunately,there are no authoritative vaccine and antiviral drug available for ASF control.African swine fever virus(ASFV)is the etiological agent of ASF.Among the ASFV proteins,p72 is the most abundant component in the virions and thus a potential target for anti-ASFV drug design.Here,we con-structed a luciferase reporter system driven by the promoter of p72,which is transcribed by the co-transfected ASFV RNA polymerase complex.Using this system,we screened over 3200 natural product compounds and obtained three potent candidates against ASFV.We further evaluated the anti-ASFV effects and proved that among the three candidates,ailanthone(AIL)inhibits the replication of ASFV at the nanomolar concentration(IC50=15 nmol/L).Our in vitro experiments indicated that the antiviral effect of AIL is associated with its inhibition of the HSP90-p23 cochaperone.Finally,we showed the antiviral activity of AIL on Zika virus and hepatitis B virus(HBV),which supports that AIL is a potential broad-spectrum antiviral agent.
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    Oridonin inhibits SARS-CoV-2 replication by targeting viral proteinase and polymerase

    Zherui ZhangHongqing ZhangYanan ZhangQiuyan Zhang...
    470-479页
    查看更多>>摘要：COVID-19 has become a global public health crisis since its outbreak in China in December 2019.Currently there are few clinically effective drugs to combat SARS-CoV-2 infection.The main protein(Mpro),papain-like protease(PLpro)and RNA-dependent RNA polymerase(RdRp)of SARS-CoV-2 are involved in the viral replication,and might be prospective targets for anti-coronavirus drug development.Here,we investigated the antiviral activity of oridonin,a natural small-molecule compound,against SARS-CoV-2 infection in vitro.The time-of-addition analysis showed that oridonin efficiently inhibited SARS-CoV-2 infection by interfering with the genome replication at the post-entry stage.Mechanistically,the inhibition of viral replication by oridonin depends on the oxidation activity of α,β-unsaturated carbonyl.Further experiments showed that oridonin not only effectively inhibited SARS-CoV-2 Mpro activity,but also had some inhibitory effects on PLpro-mediated deubiquitinating and viral polymerase-catalyzed RNA elongation activities at high concentrations.In particular,oridonin could inhibit the bat SARS-like CoV and the newly emerged SARS-CoV-2 omicron variants(BA.1 and BA.2),which highlights its potential as a pan-coronavirus antiviral agent.Overall,our data provide strong evidence that oridonin is an efficient antiviral agent against SARS-CoV-2 infection.
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