查看更多>>摘要：High mannose oligosaccharides are characteristic and essential for immune evasion of many viruses and cancer cells.They are potential targets for viral inhibition and cancer diagnosis/therapy.Particularly,high mannose-binding reagents may be a unique asset for fighting the ongoing and mutating SARS-CoV-2 virus.Lectins are prevailing reagents for saccharide binding but suffer from inadequate spec-ificity and apparent immunogenicity.Meanwhile,oth-er reagents for the same purpose,such as antibodies and aptamers,have rarely been reported.Herein,using molecularly imprinted magnetic nanoparticles as a potent platform,we report a smart selection method for fine screening of high mannose-specific aptamers.Monovalent aptamers were first effective-ly screened within eight rounds of selection.Multiva-lent aptamers,in the forms of dendritic polymer or tetrahedral DNA nanostructure(TDN),were further engineered.The aptamers exhibited high affinity to-ward the spike protein of SARS-CoV-2 and the enve-lope protein GP120 of HIV.Both the monovalent aptamer and its TDN form exhibited a certain inhibition effect to the SARS-CoV-2 pseudovirus.On the other hand,both the monovalent aptamer and its dendritic form permitted the recognition of cancer cells over normal cells.Therefore,as unprecedented reagents for broad-spectrum viral inhibition and can-cer targeting,these aptamers hold great promise for clinical treatment and diagnosis.
查看更多>>摘要：It remains a great challenge to balance the kinetic stability and intrinsic healing ability of polymer mate-rials.Here,we present an efficient strategy of using a synthetic reaction cycle to regulate the intrinsic heal-ing ability of thermodynamically stable and kinetically inert multifunctional organohydrogels.By combining a double decomposition reaction with spontaneous energy dissipation,we can construct the simplest synthetic reaction cycle that can induce a transient out-of-equilibrium state for achieving the healing of organohydrogels with kinetically locked acylhydra-zone bonds.In addition to balancing kinetic stability and healing ability,the synthetic reaction cycle also enables the polymer materials to have high tolerance to organic solvents,high ionic strength,high and low temperatures,and other harsh conditions.Therefore,the kinetically stable and healable organohydrogels remain mechanically flexible and electrically conduc-tive even down to-40 ℃ and are readily recyclable.The integration of chemical networks into healable polymers may provide novel,versatile materials for building next-generation electronic devices.
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