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中国昆虫科学（英文版）
中国昆虫科学（英文版）

王牧牧

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1672-9609

010-82672313

100080

北京海淀区北四环西路25号

中国昆虫科学（英文版）/Journal Insect ScienceCSCDCSTPCD北大核心SCI
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    A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(Froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)

    Melissa L.StarkieElizabeth V.FowlerAlexander M.PiperXiaocheng Zhu...
    1285-1295页
    查看更多>>摘要：Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distri-butions,and host ranges.The need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference colonies.While morphologically similar,adults may be separated based on subtle charac-ters;however,some characters exhibit intraspecific variability,creating overlap between the two species.Additionally,there is currently no single molecular marker or rapid diag-nostic assay that can reliably distinguish between B.neohumeralis and B.tryoni;therefore,ambiguous samples remain undiagnosed.Here we report the first molecular marker that can consistently distinguish between B.tryoni and B.neohumeralis.Our diagnostic region consists of two adjacent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene region.We confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation sys-tem(ARMS)PCR assay for rapid diagnosis of the species.The assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adjacent SNPs(AA)present in B.tryoni and the other targeting adjacent SNPs present in B.neohumeralis(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly species.Therefore,this assay is most suited to adult diagnostics where species confirma-tion is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses.
      原文链接:
    • 万方数据
    • 维普

    Heterosis effect for larval performance of fall armyworm interstrain hybrids

    Laijiao LanNicolas Nègre
    1296-1312页
    查看更多>>摘要：Spodoptera frugiperda,also known as fall armyworm(FAW),is an invasive crop pest that can feed on a variety of host plants,posing a serious threat to food security.There are two sympatric strains of FAW that are morphologically identical but described with different food preferences:the"rice strain"(SfR)and the"corn strain"(SfC).A few genetic loci exist to identify these two strains.Mitochondrial and Z-chromosome-linked haplotypes are the most used,but the biggest part of the genome displays little polymorphism between strains that could explain their adaptation to different plants.We have previously observed consistent transcription differences between the strains in both laboratory and natural populations.Therefore,we wonder if there are effects from host-strain-associated loci,maternally or paternally inherited,on FAW performance that could explain the divergence between the two FAW strains.To test this hypothesis,we first pro-duced two F1 hybrid generations(SfR ♀ × SfC ♂,SfC ♀ × SfR ♂).These reciprocal hybrids should be heterozygous for all chromosomes except for the maternally inherited mitochondrial and sexual W chromosomes.To evaluate whether plant preference is deter-mined by these genetic loci,we cultivated the two hybrids and the two parental strains in triplicate on an artificial diet and recorded several phenotypic traits such as weight over time,survival rate,emerging rate,developmental time,and sex ratio.Then,the same per-formance experiment was carried out on corn plants.Surprisingly,on the artificial diet,the two hybrid genotypes were both more performant than the two parental strains in terms of survival rate,pupal emerging rate,and developmental time,whereas they were interme-diate to the inbred parental strains in pupal weight.On the corn plant diet,both hybrid genotypes outperformed the two parental strains in larval weight.Although these asym-metrical results revealed that mitochondrial or sex-linked haplotypes alone cannot explain the performance differences,they suggested a heterosis effect in FAW.A reduction of the female number for the CR genotype and the decreased F1 offspring reproduction in both hybrids suggested the possibility of Haldane's rule,which might be explained by the dom-inance model.
      原文链接:
    • 万方数据
    • 维普

    Competitive interactions between invasive fall armyworm and Asian corn borer at intraspecific and interspecific level on the same feeding guild

    Jingfei GuoJianqin ShiHailiang HanIvan Rwomushana...
    1313-1325页
    查看更多>>摘要：Interspecific competition is an important factor in the population dynamics and geographical distribution of insect populations.Fall armyworm(FAW),Spodoptera frugiperda,an invasive species,and the Asian corn borer(ACB),Ostrinia furnacalis,a na-tive pest species are major pests on maize in China,posing a threat to maize yield and grain quality.A series of laboratory,greenhouse,and field experiments were conducted to elu-cidate the competitive interactions between FAW and ACB.In the laboratory experiments,FAW exhibited aggressive behaviors more frequently when compared with ACB,while the latter species exhibited defense behaviors more frequently.Higher intraspecific competi-tion was recorded in the FAW conspecific rather than in ACB,particularly in the 6th larval stage.FAW had a higher interspecific competitive advantage through intraguild predation over ACB,resulting in partial or complete displacement ACB when initially the ratio of the 2 species was 1∶1.The interspecific competition also had significantly influenced on the population parameters,defensive enzymes,and nutrient of these 2 species.Competitive interaction proved that the response of superoxide,catalase,and soluble protein in FAW were significantly increased,whereas the total sugar content in both species was substan-tially decreased.Survival rate,and the plant damage that co-infested by both species varied significantly among the sequential combinations under greenhouse and field conditions.FAW consistently exhibited stronger intraspecific aggression than ACB under laboratory and field conditions when co-existing on the same feeding guild.These findings contribute to efforts toward the improvement of integrated pest management programs for FAW,in decision making for invasive and native pests'management strategies to reduce the high risks of FAW and ACB outbreaks.
      原文链接:
    • 万方数据
    • 维普

    Clathrin heavy chain is involved in infection of Candidatus Liberibacter asiaticus in the host vector Diaphorina citri

    Lianjie XieXiangdong ZengMuhammad Bilal AmirXiaojin Zou...
    1326-1332页
      原文链接:
    • 万方数据
    • 维普