查看更多>>摘要：The fall armyworm(FAW),Spodoptera frugiperda,has colonized and caused consistent damage in the Eastern hemisphere.The identification of various FAW strains is essential for developing precise prevention and control measures.The triosephosphate isomerase(Tpi)gene is recognized as an effective marker closely linked to FAW subpopu-lations.However,most current studies primarily focus on the comparison of variations in specific gene sites of this gene.In this study,we conducted full-length sequencing of the Tpi genes from 5 representative FAW groups.Our findings revealed that the Tpi genes var-ied in length from 1220 to 1420 bp,with the primary variation occurring within 4 introns.Notably,the exon lengths remained consistent,at 747 bp,with 37 observed base varia-tions;however,no amino acid variations were detected.Through sequence alignment,we identified 8 stable variation sites that can be used to distinguish FAW strains in the East-ern hemisphere.Additionally,we performed strain identification on 1569 FAW samples collected from 19 provinces in China between 2020 and 2021.The extensive analysis indi-cated the absence of the rice strain in the samples.Instead,we only detected the presence of the corn strain and the Zambia strain,with the Zambia strain being distributed in a very low proportion(3.44％).Furthermore,the corn strain could be further categorized into 2 subgroups.This comprehensive study provides a valuable reference for enhancing our understanding of FAW population differentiation and for improving monitoring and early warning efforts.
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维普