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农业科学学报（英文）
中国农业科学院农业信息研究所
农业科学学报（英文）

中国农业科学院农业信息研究所

翟虎渠

月刊

2095-3119

zgnykx@mail.caas.net.cn

010-82106283 82106280

100081

北京中关村南大街12号

农业科学学报（英文）/Journal Journal of Integrative AgricultureCSCDCSTPCD北大核心SCI
查看更多>>本刊创刊于2002年，由中国农业科学院、中国农学会主办，中国农业科学院农业信息研究所承办。刊登农牧业基础科学和应用科学的研究论文，覆盖作物科学、动物科学、农业环境、农业经济与管理等领域。
正式出版
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    Ovule number as a rising star for regulating seed yield:Hope or hype

    Shahid Ullah KhanAhmad AliSumbul SaeedYonghai Fan...
    3623-3640页
    查看更多>>摘要：Rapeseed(Brassica napus L.)is the second most widely grown premium oilseed crop globally,mainly for its vegetable oil and protein meal.One of the main goals of breeders is producing high-yield rapeseed cultivars with sustainable production to meet the requirements of the fast-growing population.Besides the pod number,seeds per silique(SS),and thousand-seed weight(TSW),the ovule number(ON)is a decisive yield determining factor of individual plants and the final seed yield.In recent years,tremendous efforts have been made to dissect the genetic and molecular basis of these complex traits,but relatively few genes or loci controlling these traits have been reported thus far.This review highlights the updated information on the hormonal and molecular basis of ON and development in model plants(Arabidopsis thaliana).It also presents what is known about the hormonal,molecular,and genetic mechanism of ovule development and number,and bridges our understanding between the model plant species(A.thaliana)and cultivated species(B.napus).This report will open new pathways for primary and applied research in plant biology and benefit rapeseed breeding programs.This synopsis will stimulate research interest to further understand ovule number determination,its role in yield improvement,and its possible utilization in breeding programs.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Consensus linkage map construction and QTL mapping for eight yield-related traits in wheat using the BAAFS Wheat 90K SNP array

    Lihua LiuPingping QuYue ZhouHongbo Li...
    3641-3656页
    查看更多>>摘要：Identifying stable quantitative trait loci(QTLs)for yield-related traits across populations and environments is crucial for wheat breeding and genetic studies.Consensus maps also play important roles in wheat genetic and genomic research.In the present study,a wheat consensus map was constructed using a doubled haploid(DH)population derived from Jinghua 1×Xiaobaidongmai(JX),an F2 population derived from L43×Shanxibaimai(LS)and the BAAFS Wheat 90K SNP array single nucleotide polymorphism(SNP)array.A total of 44,503 SNP markers were mapped on the constructed consensus map,and they covered 5,437.92 cM across 21 chromosomes.The consensus map showed high collinearity with the individual maps and the wheat reference genome IWGSC RefSeq v2.1.Phenotypic data on eight yield-related traits were collected in the JX population,as well as the F2:3 and F2:4 populations of LS,in six,two and two environments,respectively,and those data were used for QTL analysis.Inclusive composite interval mapping(ICIM)identified 32 environmentally stable QTLs for the eight yield-related traits.Among them,four QTLs(QPH.baafs-4B,QKNS.baafs-4B,QTGW.baafs-4B,and QSL.baafs-5A.3)were detected across mapping populations and environments,and nine stable QTLs(qKL.baafs-1D,QPH.baafs-2B,QKNS.baafs-3D,QSL.baafs-3D,QKW.baafs-4B,QPH.baafs-5D,QPH.baafs-6A.1,QSL.baafs-6A,and QSL.baafs-6D)are likely to be new.The physical region of 17.25-44.91 Mb on chromosome 4B was associated with six yield-related traits,so it is an important region for wheat yield.The physical region around the dwarfing gene Rht24 contained QTLs for kernel length(KL),kernel width(KW),spike length(SL),and thousand-grain weight(TGW),which are either from a pleiotropic effect of Rht24 or closely linked loci.For the stable QTLs,254 promising candidate genes were identified.Among them,TraesCS5A03G1264300,TraesCS1B03G0624000 and TraesCS6A03G0697000 are particularly noteworthy since their homologous genes have similar functions for the corresponding traits.The constructed consensus map and the identified QTLs along with their candidate genes will facilitate the genetic dissection of wheat yield-related traits and accelerate the development of wheat cultivars with desirable plant morphology and high yield.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Genome-wide association study dissecting drought resistance-associated loci based on physiological traits in common bean

    Lei WuYujie ChangLanfen WangShumin Wang...
    3657-3671页
    查看更多>>摘要：Genetic improvement of drought resistance is one of the main breeding goals for common bean,so molecular markers must be identified to facilitate drought resistance breeding.In this study,we evaluated the proline,trehalose,raffinose,and stachyose contents of 210 common bean accessions under two watering conditions and found large variations in all four.The coefficients of variation ranged from 21.21%for proline content to 78.69%for stachyose content under well-watered conditions,and from 20.11%for proline content to 50.08%for trehalose content under drought stress.According to our genome-wide association analysis,32 quantitative trait loci were associated with drought resistance,seven of which overlapped with known loci.Four hotspot regions were identified at Pv01,Pv07 and Pv11.A set of candidate genes was identified,including genes encoding MYB,bZIP,bHLH,ERF,and protein kinases.Among these genes,Phvul.001G189400,Phvul.007G273000 and Phvul.008G270500 were annotated as bZIP,ERF and WRKY,respectively.These genes are reportedly involved in drought stress responses in Arabidopsis thaliana and were induced by drought stress in common bean.Significant SNPs in six candidate gene regions formed different haplotypes,and phenotypic analysis revealed significant differences among the haplotypes.These results provide new insight into the genetic basis of drought resistance in common bean and reveal candidate genes and superior natural variations that will be useful for improving common bean.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    One-time application of controlled-release bulk blending fertilizer enhances yield,quality and photosynthetic efficiency in late japonica rice

    Canping DunRui WangKailiang MiYuting Zhang...
    3672-3691页
    查看更多>>摘要：Controlled-release urea(CRU)releases nitrogen(N)at the same pace that rice takes it up,which can effectively improve N use efficiency,increase rice yield and improve rice quality.However,few studies have described the effects of CRU application on the photosynthetic rate and endogenous enzyme activities of rice.Accordingly,a two-year field trial was conducted with a total of seven treatments:CK,no N fertilizer;BBF,regular blended fertilizer;RBBF,20%N-reduced regular blended fertilizer;CRF1,70%CRU+30%regular urea one-time base application;CRF2,60%CRU+40%regular urea one-time base application;RCRF1,CRF1 treatment with 20%N reduction;and RCRF2,CRF2 treatment with 20%N reduction.Each treatment was conducted in triplicate.The results showed that the N recovery efficiency(NRE)of the controlled-release bulk blending fertilizer(CRBBF)treatments was significantly greater over the two years.There were significant yield increases of 4.1-5.9%under the CRF1 treatment and 5.6-7.6%under the CRF2 treatment compared to the BBF treatment,but the differences between the reduced-N treatments RBBF and RCRF2 were not significant.Photosynthetic rates under the CRF1 and CRF2 treatments were significantly higher than under the other treatments,and they had significantly greater RuBPCase,RuBisCO,glutamate synthase(GOGAT)and glutamine synthetase(GS)enzyme activities.Additionally,the soil NH4+-N and NO3--N contents under the CRBBF treatments were significantly higher at the late growth stage of rice,which was more in-line with the fertilizer requirements of rice throughout the reproductive period.CRBBF also led to some improvement in rice quality.Compared with the BBF and RBBF treatments,the protein contents under the CRBBF treatments were reduced but the milling,appearance,eating and cooking qualities of the rice were improved.These results showed that the application of CRBBF can improve the NRE,photosynthetic rate and endogenous enzyme activities of rice,ensuring sufficient N nutrition and photosynthetic material production during rice growth and thereby achieving improved rice yield and quality.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Assessing the yield difference of double-cropping rice in South China driven by radiation use efficiency

    Jian LuSicheng DengMuhammad ImranJingyin Xie...
    3692-3705页
    查看更多>>摘要：Double-cropping rice in South China continues to break the total yield record,but the yield potential of single-cropping rice is not being realized.Radiation use efficiency(RUE)has been singled out as an important determinant of grain yield in many cereal species.However,there is no information on whether the yield gaps in double-cropping rice involve differences in RUE.Field experiments were performed over two years to evaluate the effects of intercepted radiation(IP)and RUE on the above-ground biomass production,crop growth rate(CGR),and harvest index(HI),in four representative rice varieties,i.e.,Xiangyaxiangzhan(XYXZ),Meixiangzhan 2(MXZ2),Nanjingxiangzhan(NJXZ),and Ruanhuayoujinsi(RHYJS),during the early and late seasons of rice cultivation in South China.The results revealed that grain yield in the early season was 8.2%higher than in the late season.The yield advantage in the early season was primarily due to higher spikelets per panicle and above-ground biomass resulting from a higher RUE.The spikelets per panicle in the early season were 6.5,8.3,6.9,and 8.5%higher in XYXZ,MXZ2,NJXZ,and RHYJS,respectively,than in the late season.The higher early season grain yield was more closely related to RUE in the middle tillering stage(R2=0.34),panicle initiation(R2=0.16),and maturation stage(R2=0.28),and the intercepted photosynthetically active radiation(IPAR)in the maturation stage(R2=0.28),while the late season grain yield was more dependent on IPAR in the middle tillering stage(R2=0.31)and IPAR at panicle initiation(R2=0.23).The results of this study conclusively show that higher RUE contributes to the yield progress of early season rice,while the yield improvement of late season rice is attributed to higher radiation during the early reproductive stage.Rationally allocating the RUE of double-cropping rice with high RUE varieties or adjustments of the sowing period merits further study.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Transcriptomic and metabolomic analysis of changes in grain weight potential induced by water stress in wheat

    Yanmei GaoMaoya JingMeng ZhangZhen Zhang...
    3706-3722页
    查看更多>>摘要：The sink strength of developing ovaries in wheat determines the grain weight potential.The period from booting to the grain setting stage is critical for ovary growth and development and potential sink capacity determination.However,the underlying regulatory mechanism during this period by which the wheat plant balances and coordinates the floret number and ovary/grain weight under water stress has not been clarified.Therefore,we designed two irrigation treatments of W0(no seasonal irrigation)and W1(additional 75 mm of irrigation at the jointing stage)and analyzed the responses of the ovary/grain weight to water stress at the phenotypic,metabolomic,and transcriptomic levels.The results showed that the W0 irrigation treatment reduced the soil water content,plant height,and green area of the flag leaf,thus reducing grain number,especially for the inferior grains.However,it improved the grain weight of the superior and inferior grains as well as average grain weight at maturity,while the average ovary/grain weight and volume during-3 to 10 days after anthesis(DAA)also increased.Transcriptomic analysis indicated that the genes involved in both sucrose metabolism and phytohormone signal transduction were prominently accelerated by the W0 treatment,accompanied by greater enzymatic activities of soluble acid invertase(SAI)and sucrose synthase(Sus)and elevated abscisic acid(ABA)and indole-3-acetic acid(IAA)levels.Thus,the sucrose content decreased,while the glucose and fructose contents increased.In addition,several TaTPP genes(especially TaTPP-6)were down-regulated and the IAA biosynthesis genes TaTAR1 and TaTAR2 were up-regulated under the W0 treatment before anthesis,which further increased the IAA level.Collectively,water stress reduced the growth of vegetative organs and eliminated most of the inferior grains,but increased the ABA and IAA levels of the surviving ovaries/grains,promoting the enzymatic activity of Sus and degrading sucrose into glucose and fructose.As a result,the strong sucrose utilization ability,the enhanced enzymatic activity of SAI and the ABA-and IAA-mediated signaling jointly increased the weight and volume of the surviving ovaries/grains,and ultimately achieved the trade-offbetween ovary/grain weight and number in wheat under water stress.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Genome-wide identification,molecular evolution,and functional characterization of fructokinase gene family in apple reveal its role in improving salinity tolerance

    Jing SuLingcheng ZhuPingxing AoJianhui Shao...
    3723-3736页
    查看更多>>摘要：Fructokinase(FRK)is a regulator of fructose signaling in plants and gateway proteins that catalyze the initial step in fructose metabolism through phosphorylation.Our previous study demonstrated that MdFRK2 protein exhibit not only high affinity for fructose,but also high enzymatic activity due to sorbitol.However,genome-wide identification of the MdFRK gene family and their evolutionary dynamics in apple are yet to be reported.A systematic genome-wide analysis in this study identified a total of nine MdFRK gene members,which could phylogenetically be clustered into seven groups.Chromosomal location and synteny analysis of MdFRKs revealed that their expansion in the apple genome is primarily driven by tandem and segmental duplication events.Divergent expression patterns of MdFRKs were observed in four source-sink tissues and at five different apple fruit developmental stages,which suggested their potential crucial roles in the apple fruit development and sugar accumulation.Reverse transcription-quantitative PCR(RT-qPCR)identified candidate NaCl or drought stress responsive MdFRKs,and transgenic apple plants overexpressing MdFRK2 exhibited considerably enhanced salinity tolerance.Our results will be useful for understanding the functions of MdFRKs in the regulation of apple fruit development and salt stress response.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Suppression of CsFAD3 in a JA-dependent manner,but not through the SA pathway,impairs drought stress tolerance in tea

    Na ChangXiaotian PiZiwen ZhouYeyun Li...
    3737-3750页
    查看更多>>摘要：The growth and yield of tea plants are seriously limited by drought stress.Fatty acid desaturases(FADs)contribute to the mediation of membrane fluidity in response to different stresses,although the role of ω-3 FAD(Omega-3 fatty acid desaturase)-mediated damage induced by drought stress in tea plants is poorly understood.In this study,drought stress significantly promoted the synthesis of C18:3(linolenic acid)and the expression level of CsFAD3.Yeast experiments further demonstrated that CsFAD3 can convert C18:2 to C18:3,and that the 35S:GFP-CsFAD3 fusion protein was localized in the endoplasmic reticulum of Nicotiana benthamiana cells.CsFAD3-silenced tea leaves exhibited poor drought tolerance,with a lower Fv/Fm and a higher malondialdehyde(MDA)content than the control plants.However,transgenic 35S:CsFAD3 Arabidopsis plants showed the opposite phenotypes.In addition,the jasmonic acid(JA)content and the expression levels of CsLOX2,CsLOX4,CsAOS,CsAOC3 and CsOPR2 were significantly reduced in CsFAD3-silenced leaves under drought stress.However,no substantial difference in the salicylic acid(SA)content was detected under normal or drought conditions.An analysis of Atcoi1(JA receptor)or Atnpr1(SA receptor)mutant Arabidopsis plants in 35S:CsFAD3 backgrounds further revealed that knockout of Atcoi1 impaired the drought-tolerant phenotypes of CsFAD3 overexpression lines.Therefore,this study demonstrated that CsFAD3 plays a crucial role in drought tolerance by mediating JA pathways.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Intergeneric chromosome-specific painting reveals differential chromosomal transmission from Tripidium arundinaceum in sugarcane progeny

    Fan YuZehuai YuJin ChaiXikai Yu...
    3751-3762页
    查看更多>>摘要：Sugarcane has recently attracted increasing attention for its potential as a source of sugar and bioethanol,so increasing its yield is essential to ensure the sugar security and bioenergy production.Intergeneric hybridization is a highly efficient method to produce new genetic variants of crop plants,particularly those species with high ploidy such as sugarcane(Saccharum spp.).Tripidium arundinaceum exhibits many desirable agronomic traits,and has been widely studied to produce hybrids with improved stress tolerance and other characteristics in sugarcane breeding.However,the genetic relationship between T.arundinaceum and Saccharum species,and the individual T.arundinaceum chromosomal compositions in sugarcane hybrids are still elusive.Here we used whole-genome single-nucleotide polymorphisms(SNPs)to ascertain the phylogenetic relationships between these species and found that T.arundinaceum is more closely related to Saccharum than Sorghum,in contrast to the previous narrow genetic analyses using chloroplast DNA.Additionally,oligonucleotide(oligo)-based chromosome-specific painting derived from Saccharum officinarum was able to distinctly identify the chromosomes of T.arundinaceum.We developed the oligo-genomic in situ hybridization(GISH)system for the first time,to unveil the novel chromosome translocations and the transmission of individual T.arundinaceum chromosomes in sugarcane progeny.Notably,we discovered that the chromosomal transmission of T.arundinaceum exhibited several different inheritance modes,including n,2n,and over 2n in the BC1 progenies.Such inheritance patterns may have resulted from first division restitution(FDR)or FDR+nondisjunction of a chromosome with the sister chromatids in the second meiosis division/second division restitution(FDR+NSC/SDR)model during meiosis.These results will be of substantial benefit for the further selection of T.arundinaceum chromosomes for sugarcane genetic improvement.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据

    Rapid detection of the rice false smut fungus Ustilaginoidea virens by lateral flow strip-based recombinase polymerase amplification assay

    Jiacheng XiSanlian WanYue LiYuandi Xu...
    3763-3773页
    查看更多>>摘要：Rice false smut,caused by Ustilaginoidea virens,is a devastating disease that greatly reduces rice yield and quality.However,controlling rice false smut disease is challenging due to the unique infection mode of U.virens.Therefore,there is a need for early diagnosis and monitoring techniques to prevent the spread of this disease.Lateral flow strip-based recombinase polymerase amplification(LF-RPA)overcomes the limitations of current U.virens detection technologies,which are time-consuming,require delicate equipment,and have a high false-positive rate.In this study,we used a comparative genomics approach to identify Uv_3611,a specific gene of U.virens,as the target for the LF-RPA assay.The designed primers and probe efffectively detected the genomic DNA(gDNA)of U.virens and demonstrated no cross-reactivity with related pathogens.Under optimal conditions,the LF-RPA assay demonstrated a sensitivity of 10 pg of U.virens gDNA.Additionally,by incorporating a simplified PEG-NaOH method for plant DNA extraction,the LF-RPA assay enabled the detection of U.virens in rice spikelets within 30 min,without the need for specialized equipment.Furthermore,the LF-RPA assay successfully detected U.virens in naturally infected rice and seed samples in the field.Therefore,the LF-RPA assay is sensitive,efficient,and convenient,and could be developed as a kit for monitoring rice false smut disease in the field.
      原文链接:
    • 国家科技期刊平台
    • NETL
    • NSTL
    • 万方数据