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农业科学学报（英文）
中国农业科学院农业信息研究所
农业科学学报（英文）

中国农业科学院农业信息研究所

翟虎渠

月刊

2095-3119

zgnykx@mail.caas.net.cn

010-82106283 82106280

100081

北京中关村南大街12号

农业科学学报（英文）/Journal Journal of Integrative AgricultureCSCDCSTPCD北大核心SCI
查看更多>>本刊创刊于2002年，由中国农业科学院、中国农学会主办，中国农业科学院农业信息研究所承办。刊登农牧业基础科学和应用科学的研究论文，覆盖作物科学、动物科学、农业环境、农业经济与管理等领域。
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    The virulence regulator AbsR in avian pathogenic Escherichia coli has pleiotropic effects on bacterial physiology

    Dongfang ZhaoHaobo ZhangXinyang ZhangFengwei Jiang...
    649-668页
    查看更多>>摘要：Avian pathogenic Escherichia coli (APEC) belonging to extraintestinal pathogenic E.coli (ExPEC) can cause severe infections in extraintestinal tissues in birds and humans,such as the lungs and blood.MprA (microcin production regulation,locus A,herein renamed AbsR,a blood survival regulator),a member of the MarR (multiple antibiotic resistance regulator) transcriptional regulator family,governs the expression of capsule biosynthetic genes in human ExPEC and represents a promising druggable target for antimicrobials.However,a deep understanding of the AbsR regulatory mechanism as well as its regulon is lacking.In this study,we present a systems-level analysis of the APEC AbsR regulon using ChIP-Seq (chromatin immunoprecipitation sequencing) and RNA-Seq (RNA sequencing) methods.We found that AbsR directly regulates 99 genes and indirectly regulates 667 genes.Furthermore,we showed that:1) AbsR contributes to antiphagocytotic effects by macrophages and virulence in a mouse model for systemic infection by directly activating the capsular gene cluster;2) AbsR positively impacts biofilm formation via direct regulation of the T2SS (type Ⅱ secretion system) but plays a marginal role in virulence;and 3) AbsR directly upregulates the acid tolerance signaling system EvgAS to withstand acid stress but is dispensable in ExPEC virulence.Finally,our data indicate that the role of AbsR in virulence gene regulation is relatively conserved in ExPEC strains.Altogether,this study provides a comprehensive analysis of the AbsR regulon and regulatory mechanism,and our data suggest that AbsR likely influences virulence primarily through the control of capsule production.Interestingly,we found that AbsR severely represses the expression of the type Ⅰ-F CRISPR (clustered regularly interspaced short palindromic repeats)-Cas (CRISPR associated) systems,which could have implications in CRISPR biology and application.
      原文链接:
    • 万方数据
    • 维普

    Straw return increases crop production by improving soil organic carbon sequestration and soil aggregation in a long-term wheat-cotton cropping system

    Changqin YangXiaojing WangJianan LiGuowei Zhang...
    669-679页
    查看更多>>摘要：Straw return is a promising strategy for managing soil organic carbon (SOC) and improving yield stability.However,the optimal straw return strategy for sustainable crop production in the wheat (Triticum aestivum L.)-cotton (Gossypium hirsutum L.) cropping system remains uncertain.The objective of this study was to quantify the long-term (10 years) impact of carbon (C) input on SOC sequestration,soil aggregation and crop yields in a wheat-cotton cropping system in the Yangtze River Valley,China.Five treatments were arranged with a single-factor randomized design as follows:no straw return (Control),return of wheat straw only (Wt),return of cotton straw only (Ct),return of 50％ wheat and 50％ cotton straw (Wh-Ch) and return of 100％ wheat and 100％ cotton straw (Wt-Ct).In comparison to the Control,the SOC content increased by 8.4 to 20.2％ under straw return.A significant linear positive correlation between SOC sequestration and C input (1.42-7.19 Mg ha-1 yr-1) (P<0.05) was detected.The percentages of aggregates of sizes>2 and 1-2 mm at the 0-20 cm soil depth were also significantly elevated under straw return,with the greatest increase of the aggregate stability in the Wt-Ct treatment (28.1％).The average wheat yields increased by 12.4-36.0％ and cotton yields increased by 29.4-73.7％,and significantly linear positive correlations were also detected between C input and the yields of wheat and cotton.The average sustainable yield index (SYI) reached a maximum value of 0.69 when the C input was 7.08 Mg ha-1 yr-1,which was close to the maximum value (SYI of 0.69,C input of 7.19 Mg ha-1 yr-1) in the Wt-Ct treatment.Overall,the return of both wheat and cotton straw was the best strategy for improving SOC sequestration,soil aggregation,yields and their sustainability in the wheat-cotton rotation system.
      原文链接:
    • 万方数据
    • 维普

    Dynamic regulation of the irrigation-nitrogen-biochar nexus for the synergy of yield,quality,carbon emission and resource use efficiency in tomato

    Ping'an ZhangMo LiQiang FuVijay P.Singh...
    680-697页
    查看更多>>摘要：Integrated water and fertilizer management is important for promoting sustainable development of facility agriculture,and biochar plays an important role in guaranteeing food production,as well as alleviating water shortages and the overuse of fertilizers.The field experiment had twelve treatments and a control (CK) trial including two irrigation amounts (I1,100％ ETm;I2,60％ ETm;where ETm is the maximum evapotranspiration),two nitrogen applications (N1,360 kg ha-1;N2,120 kg ha-1) and three biochar application levels (B1,60 t ha-1;B2,30 t ha-1 and B3,0 t ha-1).A multi-objective synergistic irrigation-nitrogen-biochar application system for improving tomato yield,quality,water and nitrogen use efficiency,and greenhouse emissions was developed by integrating the techniques of experimentation and optimization.First,a coupled irrigation-nitrogen-biochar plot experiment was arranged.Then,tomato yield and fruit quality parameters were determined experimentally to establish the response relationships between irrigation-nitrogen-biochar dosage and yield,comprehensive quality of tomatoes (TCQ),irrigation water use efficiency (IWUE),partial factor productivity of nitrogen (PFPN),and net greenhouse gas emissions (NGE).Finally,a multi-objective dynamic optimization regulation model of irrigation-nitrogen-biochar resource allocation at different growth stages of tomato was constructed which was solved by the fuzzy programming method.The results showed that the application of irrigation and nitrogen to biochar promoted increase in yield,IWUE and PFPN,while it had an inhibitory effect on NGE.In addition,the optimal allocation amounts of water and fertilizer were different under different scenarios.The yield of the S1 scenario increased by 8.31％ compared to the B1I1N2 treatment;TCQ of the S2 scenario increased by 5.14％ compared to the B2I2N1 treatment;IWUE of the S3 scenario increased by 10.01％ compared to the B1I2N2 treatment;PFPN of the S4 scenario increased by 9.35％ compared to the B1I1N2 treatment;and NGE of the S5 scenario decreased by 11.23％ compared to the B2I1N1 treatment.The optimization model showed that the coordination of multiple objectives considering yield,TCQ,IWUE,PFPN,and NGE increased on average from 4.44 to 69.02％ compared to each treatment when the irrigation-nitrogen-biochar dosage was 205.18 mm,186 kg ha-1 and 43.31 t ha-1,respectively.This study provides a guiding basis for the sustainable management of water and fertilizer in greenhouse tomato production under drip irrigation fertilization conditions.
      原文链接:
    • 万方数据
    • 维普

    Manure substitution improves maize yield by promoting soil fertility and mediating the microbial community in lime concretion black soil

    Minghui CaoYan DuanMinghao LiCaiguo Tang...
    698-710页
    查看更多>>摘要：Synthetic nitrogen (N) fertilizer has made a great contribution to the improvement of soil fertility and productivity,but excessive application of synthetic N fertilizer may cause agroecosystem risks,such as soil acidification,groundwater contamination and biodiversity reduction.Meanwhile,organic substitution has received increasing attention for its ecologically and environmentally friendly and productivity benefits.However,the linkages between manure substitution,crop yield and the underlying microbial mechanisms remain uncertain.To bridge this gap,a three-year field experiment was conducted with five fertilization regimes:i) Control,non-fertilization;CF,conventional synthetic fertilizer application;CF1/2M1/2,1/2 N input via synthetic fertilizer and 1/2 N input via manure;CF1/4M3/4,1/4 N input synthetic fertilizer and 3/4 N input via manure;M,manure application.All fertilization treatments were designed to have equal N input.Our results showed that all manure substituted treatments achieved high soil fertility indexes (SFI) and productivities by increasing the soil organic carbon (SOC),total N (TN) and available phosphorus (AP) concentrations,and by altering the bacterial community diversity and composition compared with CF.SOC,AP,and the soil C:N ratio were mainly responsible for microbial community variations.The co-occurrence network revealed that SOC and AP had strong positive associations with Rhodospirillales and Burkholderiales,while TN and C:N ratio had positive and negative associations with Micromonosporaceae,respectively.These specific taxa are implicated in soil macroelement turnover.Random Forest analysis predicted that both biotic (bacterial composition and Micromonosporaceae) and abiotic (AP,SOC,SFI,and TN) factors had significant effects on crop yield.The present work strengthens our understanding of the effects of manure substitution on crop yield and provides theoretical support for optimizing fertilization strategies.
      原文链接:
    • 万方数据
    • 维普

    A hybrid CNN-LSTM model for diagnosing rice nutrient levels at the rice panicle initiation stage

    Fubing LiaoXiangqian FengZiqiu LiDanying Wang...
    711-723页
    查看更多>>摘要：Nitrogen (N) and potassium (K) are two key mineral nutrient elements involved in rice growth.Accurate diagnosis of N and K status is very important for the rational application of fertilizers at a specific rice growth stage.Therefore,we propose a hybrid model for diagnosing rice nutrient levels at the early panicle initiation stage (EPIS),which combines a convolutional neural network (CNN) with an attention mechanism and a long short-term memory network (LSTM).The model was validated on a large set of sequential images collected by an unmanned aerial vehicle (UAV) from rice canopies at different growth stages during a two-year experiment.Compared with VGG16,AlexNet,GoogleNet,DenseNet,and inceptionV3,ResNet101 combined with LSTM obtained the highest average accuracy of 83.81％ on the dataset of Huanghuazhan (HHZ,an indica cultivar).When tested on the datasets of HHZ and Xiushui 134 (XS134,a japonica rice variety) in 2021,the ResNet101-LSTM model enhanced with the squeeze-and-excitation (SE) block achieved the highest accuracies of 85.38 and 88.38％,respectively.Through the cross-dataset method,the average accuracies on the HHZ and XS134 datasets tested in 2022 were 81.25 and 82.50％,respectively,showing a good generalization.Our proposed model works with the dynamic information of different rice growth stages and can efficiently diagnose different rice nutrient status levels at EPIS,which are helpful for making practical decisions regarding rational fertilization treatments at the panicle initiation stage.
      原文链接:
    • 万方数据
    • 维普

    Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.

    Wei HuangRuyu JiaoHongtao ChengShengli Cai...
    724-730页
    查看更多>>摘要：The yellow seed trait is preferred by breeders for its potential to improve the seed quality and commercial value of Brassica napus.In the present study,we produced yellow seed mutants using a CRISPR/Cas9 system when the two BnPAP2 homologs were knocked out.Histochemical staining of the seed coat demonstrated that proanthocyanidin accumulation was significantly reduced in the pap2 double mutants and decreased specifically in the endothelial and palisade layer cells of the seed coat.Transcriptomic and metabolite profiling analysis suggested that disruption of the BnPAP2 genes could reduce the expression of structural and regulated genes in the phenylpropanoid and flavonoid biosynthetic pathways.The broad suppression of these genes might hinder proanthocyanidin accumulation during seed development,and thereby causing the yellow seed trait in B.napus.These results indicate that BnPAP2 might play a vital role in the regulatory network controlling proanthocyanidin accumulation.
      原文链接:
    • 万方数据
    • 维普

    First identification of the oxazolidinone/phenicol resistance gene optrA in Streptococcus pluranimalium worldwide

    Kuan ZhaoLongyu ZhouShixia ZhangWanjiang Zhang...
    731-734页
      原文链接:
    • 万方数据
    • 维普