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农业科学学报(英文)
中国农业科学院农业信息研究所
农业科学学报(英文)

中国农业科学院农业信息研究所

翟虎渠

月刊

2095-3119

zgnykx@mail.caas.net.cn

010-82106283 82106280

100081

北京中关村南大街12号

农业科学学报(英文)/Journal Journal of Integrative AgricultureCSCDCSTPCD北大核心SCI
查看更多>>本刊创刊于2002年,由中国农业科学院、中国农学会主办,中国农业科学院农业信息研究所承办。刊登农牧业基础科学和应用科学的研究论文,覆盖作物科学、动物科学、农业环境、农业经济与管理等领域。
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    Identification,pathogenicity,and fungicide sensitivity of Eutiarosporella dactylidis associated with leaf blight on maize in China

    Cheng GuoXiaojie ZhangBaobao WangZhihuan Yang...
    888-900页
    查看更多>>摘要:Maize(Zea mays L.)is an economically vital grain crop that is cultivated worldwide.In 2011,a maize foliar disease was detected in Lingtai and Lintao counties in Gansu Province,China.The characteristic signs and symptoms of this disease include irregular chlorotic lesions on the tips and edges of infected leaves and black punctate fruiting bodies in dead leaf tissues.Given favourable environmental conditions,this disease spread to areas surrounding Gansu.In this study,infected leaves were collected from Gansu and Ningxia Hui Autonomous Region between 2018 and 2020 to identify the disease-causing pathogen.Based on morphological features,pathogenicity tests,and multi-locus phylogenetic analysis involving internal transcribed spacer(ITS),18S small subunit rDNA(SSU),28S large subunit rDNA(LSU),translation elongation factor 1-alpha(TEF),and β-tubulin(TUB)sequences,Eutiarosporella dactylidis was identified as the causative pathogen of this newly discovered leaf blight.Furthermore,an in vitro bioassay was conducted on representative strains using six fungicides,and both fludioxonil and carbendazim were found to significantly inhibit the mycelial growth of E.dactylidis.The results of this study provide a reference for the detection and management of Eutiarosporella leaf blight.

    Improved multi-scale inverse bottleneck residual network based on triplet parallel attention for apple leaf disease identification

    Lei TangJizheng YiXiaoyao Li
    901-922页
    查看更多>>摘要:Accurate diagnosis of apple leaf diseases is crucial for improving the quality of apple production and promoting the development of the apple industry.However,apple leaf diseases do not differ significantly from image texture and structural information.The difficulties in disease feature extraction in complex backgrounds slow the related research progress.To address the problems,this paper proposes an improved multi-scale inverse bottleneck residual network model based on a triplet parallel attention mechanism,which is built upon ResNet-50,while improving and combining the inception module and ResNext inverse bottleneck blocks,to recognize seven types of apple leaf(including six diseases of alternaria leaf spot,brown spot,grey spot,mosaic,rust,scab,and one healthy).First,the 3×3 convolutions in some of the residual modules are replaced by multi-scale residual convolutions,the convolution kernels of different sizes contained in each branch of the multi-scale convolution are applied to extract feature maps of different sizes,and the outputs of these branches are multi-scale fused by summing to enrich the output features of the images.Second,the global layer-wise dynamic coordinated inverse bottleneck structure is used to reduce the network feature loss.The inverse bottleneck structure makes the image information less lossy when transforming from different dimensional feature spaces.The fusion of multi-scale and layer-wise dynamic coordinated inverse bottlenecks makes the model effectively balances computational efficiency and feature representation capability,and more robust with a combination of horizontal and vertical features in the fine identification of apple leaf diseases.Finally,after each improved module,a triplet parallel attention module is integrated with cross-dimensional interactions among channels through rotations and residual transformations,which improves the parallel search efficiency of important features and the recognition rate of the network with relatively small computational costs while the dimensional dependencies are improved.To verify the validity of the model in this paper,we uniformly enhance apple leaf disease images screened from the public data sets of Plant Village,Baidu Flying Paddle,and the Internet.The final processed image count is 14,000.The ablation study,pre-processing comparison,and method comparison are conducted on the processed datasets.The experimental results demonstrate that the proposed method reaches 98.73%accuracy on the adopted datasets,which is 1.82%higher than the classical ResNet-50 model,and 0.29%better than the apple leaf disease datasets before preprocessing.It also achieves competitive results in apple leaf disease identification compared to some state-of-the-art methods.

    A chorismate mutase from Radopholus similis plays an essential role in pathogenicity

    Sihua YangJunyi LiShuai YangShiqiao Tang...
    923-937页
    查看更多>>摘要:In the process of infecting plants,plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls.In this study,based on transcriptome data,a chorismate mutase gene of Radopholus similis(RsCM)was identified and cloned,which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females.Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells,which inhibited the pattern-triggered immunity(PTI)induced by flg22,including callose deposition and defence gene expression,and cell death induced by immune elicitors BAX,but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1.The RNA interference(RNAi)transgenic tomato of RsCM obviously inhibited the infection,pathogenicity,and reproduction of R.similis.However,the resistance of the overexpression transgenic tomato of RsCM to R.similis infection was significantly reduced,and the expression levels of two salicylic acid(SA)pathway genes(PR1 and PR5)in roots infected by the nematode were significantly down-regulated,which indicated that RsCM might be involved in the inhibition of SA pathway.The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R.similis,which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.

    Silencing transformer and transformer-2 in Zeugodacus cucurbitae causes defective sex determination with inviability of most pseudomales

    Qin MaZizhen FanPing WangSiya Ma...
    938-947页
    查看更多>>摘要:transformer is a switch gene for sex determination in many insects,which cooperates with transformer-2 that is expressed in both sexes to regulate female differentiation,particularly in dipterans.Zeugodacus cucurbitae(Coquillett)is a very destructive pest worldwide,however,its sex determination pathway remains largely uncharacterized.Here,we show that the female sex ratio is sharply reduced with knockdown of either transformer or transformer-2 by RNA interference in early embryos of Z.cucurbitae.Most of the males grown from the embryos with transient transformer and transformer-2 suppression mated with wild-type females and produced mixed sex progeny,with one exception that produced only female progeny,and all of the few remaining males failed to mate with wild-type females and produced no progeny.The exceptional male and those males with mating failure were XX pseudomales as determined by the detection of Y chromosome-linked Maleness-on-the-Y,indicating that most XX pseudomales are not viable.The phenotypes of transformer and transformer-2 suggest that they play a key role in regulating sex determination and are required for female sexual development of Z.cucurbitae.Our results will be beneficial to the understanding of sex determination in Z.cucurbitae and can facilitate the development of genetic sexing strains for its biological control.

    Acaricidal effect of the antimicrobial metabolite xenocoumacin 1 on spider mite control

    Jiaxing WeiHong YanJie RenGuangyue Li...
    948-959页
    查看更多>>摘要:The two-spotted spider mite,Tetranychus urticae Koch,is one of the most harmful pests in many agroecosystems worldwide.To effectively manage this pest,there is an urgent need to develop novel bio-active acaricides that support integrated pest management strategies targeting T.urticae.In this study,we explored the acaricidal effects of xenocoumacin 1(Xcn1)on T.urticae and its predator Neoseiulus californicus using the highly purified compound.Xcn1 was extracted and purified from the cell-free supernatant of the Xenorhabdus nematophila CB6 mutant constructed by the easy promoter activated compound identification(easyPACId)method.When the concentration of Xcn1 exceeded 100 μg mL-1,the survival rate of spider mite adults declined to below 40%and the fecundity was decreased by 80%at six days post-application.At concentrations of 25 and 50 μg mL-1,Xcn1 significantly impeded spider mite development by inhibiting the molt.However,neither concentration had any adverse effects on the survival or reproduction of the predatory mite N.californicus.The results from laboratory and semi-field experiments consistently demonstrated the effectiveness of the antimicrobial metabolite Xcn1 in controlling pest mites at both the molecular and physiological levels.Our study offers a promising possibility that combines the compatible biocontrol agents of Xcn1 and predatory mites for integrated pest mite control.

    circRNA3669 promotes goat endometrial epithelial cells proliferation via miR-26a/RCN2 to activate PI3K/AKT-mTOR and MAPK pathways

    Xiaorui LiuJiuzeng CuiMengyao WeiXiaofei Wang...
    960-974页
    查看更多>>摘要:The development of receptive endometrium(RE)from pre-receptive endometrium(PE)for successful embryo implantation is a complex dynamic process in which the morphology and physiological states of the endometrial epithelium undergo a series of significant changes,including cell proliferation and apoptosis.However,the molecular mechanisms are not yet fully understood.In this study,a higher circRNA3669 level was observed in PE than in RE of goats.Functional assays revealed that this overexpression promoted the proliferation of goat endometrial epithelial cells(GEECs)by activating the expression of genes related to the PI3K/AKT-mTOR and MAPK pathways,thereby inhibiting apoptosis in vitro.Furthermore,circRNA3669 functioned as a competing endogenous RNA(ceRNA)to upregulate Reticulocalbin-2(RCN2)expression at the post-transcriptional level by interacting with and downregulating miR-26a in GEECs.In addition,RCN2,which is highly expressed in the PE of goats,was found to be regulated by β-estradiol(E2)and progesterone(P4).Our results demonstrated that RCN2 also affected the key proteins PI3K,AKT,mTOR,JNK,and P38 in the PI3K/AKT-mTOR and MAPK pathways,thereby facilitating GEECs proliferation and suppressing their apoptosis in vitro.Collectively,we constructed a new circRNA3669-miR-26a-RCN2 regulatory network in GEECs,which further provides strong evidence that circRNA could potentially play a crucial regulatory role in the development of RE in goats.

    Assessing the conservation impact of Chinese indigenous chicken populations between ex-situ and in-situ using genome-wide SNPs

    Wenting LiChaoqun GaoZhao CaiSensen Yan...
    975-987页
    查看更多>>摘要:Conservation programs require rigorous evaluation to ensure the preservation of genetic diversity and viability of conservation populations.In this study,we conducted a comparative analysis of two indigenous Chinese chicken breeds,Gushi and Xichuan black-bone,using whole-genome SNPs to understand their genetic diversity,track changes over time and population structure.The breeds were divided into five conservation populations(GS1,2010,ex-situ;GS2,2019,ex-situ;GS3,2019,in-situ;XB1,2010,in-situ;and XB2,2019,in-situ)based on conservation methods and generations.The genetic diversity indices of three conservation populations of Gushi chicken showed consistent trends,with the GS3 population under in-situ strategy having the highest diversity and GS2 under ex-situ strategy having the lowest.The degree of inbreeding of GS2 was higher than that of GS1 and GS3.Conserved populations of Xichuan black-bone chicken showed no obvious changes in genetic diversity between XB1 and XB2.In terms of population structure,the GS3 population were stratified relative to GS1 and GS2.According to the conservation priority,GS3 had the highest contribution to the total gene and allelic diversity in GS breed,whereas the contribution of XB1 and XB2 were similar.We also observed that the genetic diversity of GS2 was lower than GS3,which were from the same generation but under different conservation programs(in-situ and ex-situ).While XB1 and XB2 had similar levels of genetic diversity.Overall,our findings suggested that the conservation programs performed in ex-situ could slow down the occurrence of inbreeding events,but could not entirely prevent the loss of genetic diversity when the conserved population size was small,while in-situ conservation populations with large population size could maintain a relative high level of genetic diversity.

    Porcine enteric alphacoronavirus infection increases lipid droplet accumulation to facilitate the virus replication

    Qi GaoYongzhi FengTing GongDongdong Wu...
    988-1005页
    查看更多>>摘要:Coronaviruses are widely transmissible between humans and animals,causing diseases of varying severity.Porcine enteric alphacoronavirus(PEAV)is a newly-discovered pathogenic porcine enteric coronavirus in recent years,which causes watery diarrhea in newborn piglets.The host inflammatory responses to PEAV and its metabolic regulation mechanisms remain unclear,and no antiviral studies have been reported.Therefore,we investigated the pathogenic mechanism and antiviral drugs of PEAV.The transcriptomic analysis of PEAV-infected host cells revealed that PEAV could upregulate lipid metabolism pathways.In lipid metabolism,steady-state energy processes,which can be mediated by lipid droplets(LDs),are the main functions of organelles.LDs are also important in viral infection and inflammation.In infected cells,PEAV increased LD accumulation,upregulated NF-κB signaling,promoted the production of the inflammatory cytokines IL-1β and IL-8,and induced cell death.Inhibiting LD accumulation with a DGAT-1 inhibitor significantly inhibited PEAV replication,downregulated the NF-κB signaling pathway,reduced the production of IL-1β and IL-8,and inhibited cell death.The NF-κB signaling pathway inhibitor BAY11-7082 significantly inhibited LD accumulation and PEAV replication.Metformin hydrochloride also exerted anti-PEAV effects and significantly inhibited LD accumulation,downregulated the NF-κB signaling pathway,reduced the production of IL-1β and IL-8,and inhibited cell death.LD accumulation in the lipid metabolism pathway therefore plays an important role in the replication and pathogenesis of PEAV,and metformin hydrochloride inhibits LD accumulation and the inflammatory response to exert anti-PEAV activity and reducing pathological injury.These findings contribute new targets for developing treatments for PEAV infections.

    Combining field data and modeling to better understand maize growth response to phosphorus(P)fertilizer application and soil P dynamics in calcareous soils

    Weina ZhangZhigan ZhaoDi HeJunhe Liu...
    1006-1021页
    查看更多>>摘要:We used field experimental data to evaluate the ability of the agricultural production system model(APSIM)to simulate soil P availability,maize biomass and grain yield in response to P fertilizer applications on a fluvo-aquic soil in the North China Plain.Crop and soil data from a 2-year experiment with three P fertilizer application rates(0,75 and 300 kg P2O5 ha-1)were used to calibrate the model.Sensitivity analysis was carried out to investigate the influence of APSIM SoilP parameters on the simulated P availability in soil and maize growth.Crop and soil P parameters were then derived by matching or relating the simulation results to observed crop biomass,yield,P uptake and Olsen-P in soil.The re-parameterized model was further validated against 2 years of independent data at the same sites.The re-parameterized model enabled good simulation of the maize leaf area index(LAI),biomass,grain yield,P uptake,and grain P content in response to different levels of P additions against both the calibration and validation datasets.Our results showed that APSIM needs to be re-parameterized for simulation of maize LAI dynamics through modification of leaf size curve and a reduction in the rate of leaf senescence for modern stay-green maize cultivars in China.The P concentration limits(maximum and minimum P concentrations in organs)at different stages also need to be adjusted.Our results further showed a curvilinear relationship between the measured Olsen-P concentration and simulated labile P content,which could facilitate the initialization of APSIM P pools in the NCP with Olsen-P measurements in future studies.It remains difficult to parameterize the APSIM SoilP module due to the conceptual nature of the pools and simplified conceptualization of key P transformation processes.A fundamental understanding still needs to be developed for modelling and predicting the fate of applied P fertilizers in soils with contrasting physical and chemical characteristics.

    Irrigation and nitrogen fertiliser optimisation in protected vegetable fields of northern China:Achieving environmental and agronomic sustainability

    Bingqian FanYitao ZhangOwen FentonKaren Daly...
    1022-1033页
    查看更多>>摘要:Globally,sub-optimal use of nitrogen(N)fertiliser and elevated N irrigation groundwater have led to high leached nitrate(NO3-)losses from protected vegetable field systems.Optimising fertiliser and irrigation management in different soil types is crucial to reduce future N loads from such systems.The present 4-year study examined leached N loads from lysimeter monitoring arrays set up across 18 protected vegetable system sites encompassing the dominant soil types of northern China.The treatments applied at each field site were:1)a high N and high irrigation input treatment(HNHI);2)a low N but high irrigation input treatment(LNHI)and 3)a low N with low irrigation input treatment(LNLI).Results showed that the mean annual leached total nitrogen loads from the HNHI,LNHI and LNLI treatments were 325,294 and 257 kg N ha-1 in the fluvo-aquic soil,114,100 and 78 kg N ha-1 in the cinnamon soil and 79,68 and 57 kg N ha-1 in the black soil,respectively.The N dissolved in irrigation water in the fluvo-aquic soil areas was 8.26-fold higher than in the cinnamon areas.A structural equation model showed that N fertiliser inputs and leaching water amounts explained 14.7 and 81.8%of the variation of leached N loads,respectively.Correspondingly,reducing irrigation water by 21.5%decreased leached N loads by 20.9%,while reducing manure N and chemical N inputs by 22 and 25%decreased leached N loads by only 9.5%.This study highlights that protected vegetable fields dominated by fluvo-aquic soil need management to curtail leached N losses in northern China.