首页期刊导航|中华航海医学与高气压医学杂志
期刊信息/Journal information
中华航海医学与高气压医学杂志
中华航海医学与高气压医学杂志

褚新奇

双月刊

1009-6906

zhhh@chinajournal.net.cn

021-81883316

200433

上海市翔殷路880号

中华航海医学与高气压医学杂志/Journal Chinese Journal of Nautical Medicine and Hyperbaric MedicineCSCDCSTPCD北大核心
查看更多>>1994年创刊,中国科学技术协会主管,中华医学会主办。本刊是我国航海医学领域、高压氧医学与潜水医学领域唯一的专业性科技核心期刊和统计源期刊。本刊已被美国《化学文摘》(CA)、俄罗斯《文摘杂志》(AJ)、国内各大数据库收录,主要刊发航海环境医学、潜水医学、航海临床医学、高压氧医学、航海医学保障与卫生防疫、航海医学心理学、航海人-机-环系统工程学、医学教育等方面的文章。主要读者对象为从事航海医学与高压氧医学治疗、教学、科研、管理等领域的高、中级人员。
正式出版
收录年代

    高压氧辅助健脾解毒方处理对体外培养人结肠癌SW620细胞恶性生物学行为的影响

    刘亮韩秀庆杜庆慧张翔云...
    101-106页
    查看更多>>摘要:目的 探讨高压氧(HBO)辅助健脾解毒方处理对体外培养人结肠癌SW620细胞恶性生物学行为的影响及其作用机制。 方法 体外培养人结肠癌SW620细胞完成后,按照实验设计分成4组:对照组、HBO组、健脾解毒方组和联合组。对照组SW620细胞不作如何处理,HBO组予以0.25 MPa HBO处理,健脾解毒方组予以1.50 mg/ml健脾解毒方制剂处理,联合组予以1.50 mg/ml健脾解毒方剂处理后再予以0.25 MPa HBO处理。首先在200倍倒置显微镜下观察SW620细胞形态的变化,采用MTT法检测SW620细胞的增殖能力,利用Hoechst 33258染色法检测SW620细胞凋亡情况,划痕实验检测SW620细胞迁移能力,Western blotting实验检测SW620细胞内凋亡蛋白的表达水平。 结果 处理48 h后,倒置显微镜下发现健脾解毒方组SW620细胞密度轻度减少,悬浮细胞稍微增多;联合组SW620细胞密度明显减少,部分细胞皱缩变圆,悬浮细胞明显增多(P<0.05)。Hoechst 33258荧光染色发现对照组和HBO组SW620细胞核形态和数量变化不明显;健脾解毒方组SW620细胞核呈致密浓染,形态异常,颜色发白,出现部分细胞凋亡;联合组SW620细胞核更加致密浓染,凋亡细胞明显增多(P<0.01);健脾解毒方组和联合组SW620细胞迁移能力受到明显抑制,联合组划痕愈合率明显高于健脾解毒方组(35.4%vs.48.9%,P<0.05);对照组、HBO组、健脾解毒方组SW620细胞G1期、S期和G2期变化不明显(P>0.05);而联合组SW620细胞G1期明显降低,S期明显增高(P<0.05),而G2期变化不明显(P>0.05);与对照组、健脾解毒方组、HBO组比较,联合组SW620细胞内凋亡蛋白Bax、caspase-3、Cytochrome C表达水平明显升高,Bcl-2蛋白表达明显降低(P<0.01)。 结论 HBO辅助健脾解毒方处理对SW620 细胞具有抑制细胞增殖、诱导细胞凋亡及抑制细胞迁移的作用,其作用机制可能与下调Bcl-2蛋白表达,提高Bax、caspase-3、Cytochrome C蛋白表达水平有关。 Objective To investigate the effect of Jianpi Jiedu Formula assisted by hyperbaric oxygen(HBO)on malignant biological behavior of SW620 human colon cancer cells cultured in vitro and its mechanism of action. Methods SW620 human colon cancer cells were cultured in vitro and divided into four groups according to the experimental design:control group,HBO group,Jianpi Jiedu Formula group,and combination group. The control group was not treated with any intervention,the HBO group was treated with 0.25 MPa HBO,the Jianpi Jiedu Formula group was treated with 1.50 mg/mL Jianpi Jiedu Formula preparation,and the combination group was treated with 1.50 mg/mL Jianpi Jiedu Formula preparation followed by 0.25 MPa HBO. The morphological changes of SW620 cells were observed under a 200× inverted microscope. MTT assay was used to access the proliferation ability of SW620 cells. Hoechst 33258 staining was conducted to detect the apoptosis of SW620 cells. Scratch test was used to evaluate the migration ability of SW620 cells. Western blotting was applied to measure the expression level of apoptin in SW620 cells. Results Forty-eight hours after treatment,in the Jianpi Jiedu Formula group,the density of SW620 cells was slightly decreased and the suspended cells were slightly increased under inverted microscope;in the combination group,the density of SW620 cells was significantly decreased,some cells were shrunk and rounded,and the number of suspended cells was significantly increased(P<0.05). Hoechst 33258 fluorescence staining showed no significant change in the morphology and the number of SW620 cell nuclei in the control group and the HBO group. The cell nuclei of SW620 cells in the Jianpi Jiedu Formula group were dense and densely stained,with abnormal morphology,white in color,and partial apoptosis,while those cell nuclei in the combination group were much denser and densely stained,with a significant increase in apoptotic cells(P<0.01). The cell migration abilities of SW620 cells in the Jianpi Jiedu Formula group and the combination group were significantly inhibited,and the scratch healing rate in the combination group was significantly higher than that in the Jianpi Jiedu Formula group(35.4% vs. 48.9%,P<0.05). There was no significant change in G1 phase,S phase,and G2 phase of SW620 cells in the control group,the HBO group,and the Jianpi Jiedu Formula group(P>0.05). In the combination group,SW620 cells were significantly decreased in G1 phase and significantly increased in S phase(P<0.05),but no significant change in G2 phase(P>0.05). Compared with the control group,the Jianpi Jiedu Formula group,and the HBO group,the expression levels of apoptin Bax,caspase-3,and Cytochrome C in SW620 cells in the combination group were significantly increased,while the expression of Bcl-2 protein was significantly decreased(P<0.01). Conclusion Jianpi Jiedu Formula assisted by HBO can inhibit cell proliferation,induce apoptosis,and inhibit cell migration of SW620 cells. The mechanism of action may be related to the down-regulating the expression of Bcl-2 protein and enhancement of the expression levels of Bax,caspase-3,and Cytochrome C proteins.

    人结肠癌SW620细胞高压氧健脾解毒方体外培养恶性生物学行为

    高压氧联合神经内镜血肿清除术治疗对高血压脑出血患者炎症反应、神经功能及预后的影响

    周建军谢志斌黄俊龙卢靖...
    107-111页
    查看更多>>摘要:目的 探讨高压氧(HBO)联合神经内镜血肿清除术治疗对高血压脑出血(HICH)患者炎症反应、神经功能及预后的影响。 方法 选取2019年1月至2021年12月在福建龙岩市第一医院诊治的90例HICH患者作为研究对象,按照治疗方法将患者分为观察组和对照组,每组45例。2组患者均给予神经内镜血肿清除术以及常规康复治疗,观察组在对照组的治疗基础上给予HBO治疗。比较2组患者治疗前后血清肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)、超敏C反应蛋白(hs-CRP)、神经元特异性烯醇化酶(NSE)、脑源性神经营养因子(BDNF)、神经肽Y(NPY)水平、美国国立卫生研究院卒中量表(NIHSS)评分和随访6个月后的预后情况。 结果 治疗前,2组患者的血清TNF-α、IL-6、hs-CRP、NSE、BDNF、NPY水平和NIHSS评分比较,差异无统计学意义(P>0.05)。与治疗前比较,治疗后2组患者的血清TNF-α、IL-6、hs-CRP、NSE、NPY水平和NIHSS评分明显降低,且观察组降低更明显;血清BDNF水平明显升高,且观察组升高更明显,差异有统计学意义(P<0.05)。2组患者均未发生死亡。观察组患者预后恢复优于对照组,差异有统计学意义(P<0.05)。 结论 HBO联合神经内镜血肿清除术治疗HICH患者,可有效减轻患者的炎症反应,改善脑组织损伤程度、神经功能和预后。 Objective To investigate the effect of hyperbaric oxygen(HBO)combined with neuroendoscopic hematoma evacuation on inflammatory response,neurological function,and prognosis in patients with hypertensive intracerebral hemorrhage(HICH). Methods A total of 90 patients with HICH who were diagnosed and treated in the Longyan First Hospital of Fujian province from January 2019 to December 2021 were selected as the research objects. According to the treatment methods,the patients were divided into observation group and control group,with 45 cases in each group. Both groups were given neuroendoscopic hematoma evacuation and conventional rehabilitation treatment,and the observation group was given HBO treatment on the basis of the treatment of the control group. The levels of serum tumor necrosis factor‐α(TNF-α),interleukin‐6(IL‐6),high‐sensitivity C‐reactive protein(hs‐CRP),neuron‐specific enolase(NSE),brain-derived neurotrophic factor(BDNF),neuropeptide Y(NPY),National Institutes of Health Stroke Scale(NIHSS)scores,and prognoses after six months of follow‐up were compared between the two groups before and after treatment. Results Before treatment,there was no significant difference being found in the comparison of the serum levels of TNF‐α,IL‐6,hs‐CRP,NSE,BDNF,NPY,and NIHSS score between the two groups,with no statistically significant difference(P>0.05). Compared with those before treatment,the levels of serum TNF-α,IL‐6,hs‐CRP,NSE,NPY,and NIHSS scores in the two groups significantly decreased after treatment,and the decreases in the observation group were more obvious;the level of serum BDNF significantly increased,and the increase in the observation group was more obvious,the differences were statistically significant(P<0.05). No death occurred in the two groups. The prognosis of the observation group was better than that of the control group,and the difference was statistically significant(P<0.05). Conclusion HBO combined with neuroendoscopic hematoma evacuation can effectively alleviate the inflammatory response,improve the degree of brain tissue damage,neurological function,and prognosis of patients with HICH.

    高血压脑出血高压氧神经内镜血肿清除术炎症反应神经功能预后

    海军某水面舰艇部队女性舰员生殖健康状况及相关知识的调查研究

    阴雷王国栋孔乐乐杨潜...
    112-115页
    查看更多>>摘要:目的 了解女性舰员生殖健康状况及生殖健康知识掌握情况,为有针对性开展医疗专科保障及科普宣教提供参考。 方法 2022年4月便利选取53名海军某水面舰艇部队女性舰员,采用自制的生殖健康状况问卷(包括一般资料、生殖系统疾病及症状、生殖健康素养及生殖健康科普需求)进行调查。 结果 53名女性舰员中,月经不调、痛经、尿路感染和生殖系统感染的发生率分别为37.3%、34.0%、11.3%和9.4%;部分女性舰员对于一些重要的女性生殖健康知识缺乏了解,目前舰艇上的生活卫生条件和医疗保障服务尚未能完全满足女性舰员的需求,全体女性舰员都认同生殖系统疾病对于女性身体和心理健康有重要影响,同时积极要求在部队开展与女性健康密切相关的常见生殖系统疾病和症状知识的科普宣教。 结论 女性舰员群体中,生殖健康状况和健康需求应得到重视,需要进一步加强生殖健康科普宣传工作,同时提供更有针对性的医疗卫生保障服务。 Objective To investigate the reproductive health status and command of knowledge about reproductive health among female sailors,so as to provide reference for carrying out specialized medical support and science popularization and health education. Methods A total of 53 female sailors of a naval surface ship were selected as study subjects in April of 2022 and surveyed by a self‐made reproductive health questionnaire(on demographic information,reproductive system diseases and symptoms,reproductive health qualities,and need for reproductive science popularization and health education). Results Among the 53 female sailors,the incidences of irregular menstruation,dysmenorrhea,urinary tract infection,and reproductive system infection were 37.3%,34.0%,11.3%,and 9.4%,respectively. Some of them lacked some important knowledge of female reproductive health. At present,the onboard living hygiene conditions and medical support services have not fully met the needs of female crew members. All the female sailors agreed that reproductive system diseases can significantly affect the physical and mental health of females,and they actively demanded reproductive science popularization and health education of common reproductive system diseases and symptoms related to female health. Conclusion Great attention should be paid to the status and needs of reproductive health of the female sailors,science popularization and health education of reproductive health should be further promoted,and targeted medical health support service should be provided.

    女性舰员生殖健康健康教育卫勤保障

    社会心理因素对远洋捕捞渔民功能性胃肠疾病发生的影响分析

    董燕妮张传涛张梦云王映红...
    116-119页
    查看更多>>摘要:目的 探讨远洋捕捞渔民功能性胃肠病(FGID)的发病情况及社会心理因素的影响。 方法 采用整群抽样法选取2022年5月至8月休渔期山东荣成石岛港9家远洋渔业捕捞公司入石岛人民医院体检且调查资料完整的1 420名渔民作为调查对象,依据罗马Ⅳ诊断标准,最终确诊412例FGID患者为研究组,将无FGID的1 008名渔民作为对照组。由专门培训的调查人员统计所有渔民FGID的发生率及研究组人群FGID类型的分布情况。采用症状自评量表-90(SCL-90)、艾森克个性问卷(EPQ)、生活事件量表(LES)、社会支持评定量表(SSRS)、吸烟原因问卷(RRSQ)和酒精使用问卷(ADS)评价2组渔民心理因素、社会支持及生活事件对FGID发生的影响。 结果 1 420名调查对象中,412例符合FGID诊断标准,总发生率为29.01%。研究组人群SCL-90因子中躯体化、强迫、人际关系、抑郁、焦虑、恐怖评分明显高于对照组(P<0.05或P<0.01)。研究组人群负性事件量评分、生活事件总量评分、神经质评分、RRSQ评分和ADS评分明显高于对照组,客观支持评分和社会支持总分明显低于对照组,差异均有统计学意义(P<0.05或P<0.01)。 结论 远洋捕捞渔民FGID发生率较高,其主要受心理因素、社会支持及生活事件的影响,与渔民所处的远洋客观环境和职业特殊性有关。

    远洋捕捞渔民功能性胃肠病社会心理因素

    海勤人员视力状况和眼科疾病调查分析

    刘菲宋明芳朱承超张寓景...
    119-121页
    查看更多>>摘要:目的 对不同岗位海勤人员(潜水员、艇员、舰员)的视力状况和眼科疾病进行调查研究,为海勤人员视力适应性标准和鉴定提供建议。 方法 对303名海勤人员进行年度眼科体检鉴定,统计因视力不合格或眼科疾病导致的鉴定结论为待定的数量并分析原因。 结果 303名海勤人员眼科体检结果中裸眼视力≥5.0者共230人(80.5%),4.6~4.9者共63人(20.8%),<4.6者共10人(3.3%)。眼科疾病有屈光不正、老视、色弱、色盲、准分子激光原位角膜磨镶术(LASIK)术后、角膜云翳、先天性白内障和高眼压症,其中屈光不正发病率最高,为24.1%(73/303)。体检结论为待定的共18人(5.9%),待定的主要原因有屈光不正10人(55.6%),LASIK术后6人(33.3%),色弱、色盲各1人(11.1%)。 结论 海勤人员最常见的眼科疾病为屈光不正,导致眼科鉴定待定结论的主要原因是屈光不正和LASIK手术史。

    海勤人员视力标准卫勤保障

    单人水下载人运载器运行控制过程中潜水技术的合理运用

    李志辉彭军伟孙永军林洁...
    121-123页
    查看更多>>摘要:潜水员搭乘单人水下载人运载器在水下运行过程中,容易发生浮力控制失当、稳性失控、操作失误及设备故障等问题。潜水员应合理运用水下方向判断与掌握、浮力控制、稳性保持等潜水技术,及时准确处置紧急情况,确保潜水安全。

    潜水技术运载器控制

    海上核应急医学救援洗消方式的探讨

    李焕敬张大光耿斌王映红...
    124-126页
    查看更多>>摘要:核与辐射突发事件发生时,释放出的放射性物质给人类带来不可估量的损失和危害,核应急医学救援刻不容缓,尤其是在海上对污染伤员进行集约高效的洗消,尽最大限度减少对伤员的伤害显得尤为重要。笔者多年参与海上各种舰船的核应急医学救援演习活动,总结了海上核事故放射性物质的污染危害和相关救援环节,梳理了核应急医学救援洗消去污的原则与方法、特点难点,并积极探讨海上核应急医学救援洗消的处置方式,努力提高海上核应急医疗救援及洗消能力。

    核应急医学救援海上洗消

    潜艇艇员快速上浮脱险系列课程教学设计方案探讨

    牛振刘平小林洁孙永军...
    126-128页
    查看更多>>摘要:根据多年快速上浮脱险训练经验积累,结合快速上浮脱险训练实际,编制完成了由理论课程、实作课程和考评方法等内容组成的快速上浮脱险系列课程教学设计方案,完善了课程体系,形成了科学合理的应急处置程序,建立了规范的考评方法,提出了课程教学设计方案在潜艇部队实际应用的建议。

    潜艇艇员快速上浮脱险课程教学设计

    预防性护理模式联合高压氧治疗重型颅脑损伤术后患者的观察与分析

    佟洋李艳春解宏伟陈华鑫...
    128-131页
    查看更多>>摘要:目的 比较不同护理模式联合高压氧(HBO)治疗应用于重型颅脑损伤(STBI)术后患者的临床效果。 方法 选取2021年3月至2022年3月某医院重症医学科收治的112例STBI术后患者作为研究对象,随机分为对照组与观察组,每组各56例。对照组给予常规护理,观察组在常规护理的基础上给予预防性护理,所有患者均联合使用HBO治疗,比较2组患者术后并发症发生率、格拉斯哥昏迷(GCS)评分、焦虑自评量表(SAS)、抑郁自评量表(SDS)评分以及护理满意率。 结果 STBI患者术后并发症总体发生率为65.18%(73/112),对照组和观察组患者术后并发症发生率分别为89.29%(50/56)、41.07%(23/56),差异有统计学意义(P<0.01)。术后并发症中颅内血肿为主要发生类型,占41.10%(30/73)。观察组患者GCS评分和护理满意率高于对照组、SAS和SDS评分低于对照组,差异均有统计学意义(均P<0.01)。 结论 重症监护室STBI患者术后开展预防性护理联合HBO治疗,有助于降低患者术后并发症发生率,改善患者术后昏迷状态,提高患者出院时护理满意率,具有较高的临床应用价值。

    预防性护理高压氧重型颅脑损伤术后

    饱和潜水技术培训重点课程设计与应用

    林洁彭军伟李志辉孙永军...
    132-133页
    查看更多>>摘要:针对饱和潜水技术培训需求,以潜水员饱和潜水技术教学训练方案为基础,通过教学对象与课程定位分析、教学内容和教学模式设计,突出饱和潜水技术培训的重点内容,形成饱和潜水技术培训重点课程设计方案。应用本课程完成海军某部3批次156名潜水员的饱和潜水技术培训,全员参加了200 m以浅的海上饱和潜水任务。

    饱和潜水课程设计理论课程实作课程