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植物学报（英文版）
植物学报（英文版）

刘春明

月刊

1672-9072

jipb@ibcas.ac.cn

010-62836133，010-62836563

100093

北京香山南辛村20号中科院植物所内

植物学报（英文版）/Journal Journal of Integrative Plant BiologyCSCDCSTPCD北大核心SCI
查看更多>>本学报是植物学综合性学术期刊。国外发行与交换达40多个国家和地区。国际标准A4大16开铜版纸印刷。本学报力争全面反映我国植物科学的最新研究成果，关注国际热点、新的学科生长点、前沿研究课题，重视报道重要的应用基础研究。主要栏目有植物生理生化、植物遗传学和分子生物学、植物生殖生物学、结构植物学、植物化学与资源植物学、植物系统与进化、植物生态学、古植物学的原始研究论文、综述和快讯。
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    The BTB/TAZ domain-containing protein CmBT1-mediated CmANR1 ubiquitination negatively regulates root development in chrysanthemum

    Lian-Da DuZhang-Ji GuanYan-Hong LiuHui-Dong Zhu...
    285-299页
    查看更多>>摘要：Roots are fundamental for plants to adapt to variable environmental conditions.The develop-ment of a robust root system is orchestrated by numerous genetic determinants and,among them,the MADS-box gene ANR1 has garnered substantial attention.Prior research has demon-strated that,in chrysanthemum,CmANR1 positively regulates root system development.Nevertheless,the upstream regulators involved in the CmANR1-mediated regulation of root devel-opment remain unidentified.In this study,we successfully identified bric-a-brac,tramtrack and broad(BTB)and transcription adapter putative zinc finger(TAZ)domain protein CmBT1 as the interacting partner of CmANR1 through a yeast-two-hybrid(Y2H)screening library.Furthermore,we validated this physical interaction through bimolecular fluorescence complementation and pull-down assays.Functional assays revealed that CmBT1 exerted a negative influence on root development in chrysanthemum.In both in vitro and in vivo assays,it was evident that CmBT1 mediated the ubiquitination of CmANR1 through the ubiquitin/26S proteasome pathway.This ubiquitination subsequently led to the degrada-tion of the CmANR1 protein and a reduction in the transcription of CmANR1-targeted gene CmPIN2,which was crucial for root development in chrysanthemum.Genetic analysis suggested that CmBT1 modulated root development,at least in part,by regulating the level of CmANR1 protein.Collectively,these findings shed new light on the regulatory role of CmBT1 in degrading CmANR1 through ubiquitination,thereby repressing the expression of its targeted gene and inhibiting root development in chrysanthemum.
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