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中医杂志（英文版）
中医杂志（英文版）

Cao Hong-xin

季刊

0255-2922

info@journaljtcm.com;jtcm@188.com

010-64050201

100700

北京东直门内南小街16号

中医杂志（英文版）/Journal Journal of Traditional Chinese MedicineCSCDSCI
正式出版
收录年代

    Role of toll-like receptor 4/mutant myeloid differentiation primary response 88/nuclear factor kappa-B mediated inflammation in diabetes mellitus with Northwest dryness syndrome

    DENG DeqiangXIAO YanMA DanQIU Jinling...
    963-973页
    查看更多>>摘要：OBJECTIVE:To investigate the role of toll-like receptor 4(TLR4)/mutant myeloid differentiation primary response 88(MyD88)/nuclear factor kappa-B(NF-κB)signaling pathway-mediated inflammation in diabetes mellitus with Northwest dryness syndrome.METHODS:Rats were randomly divided into the normal control,type 2 diabetes(T2DM)model,Northwest dryness syndrome+T2DM(Northwest dryness),and simple internal dampness+T2DM(internal dampness)groups.Enzyme-linked immunosorbent assay was used to detect biochemical indexes and inflammatory factors.The histopathological observation was performed.Quantitative real-time polymerase chain reaction and Western blot analysis were used to detect the mRNA and protein expression levels,respectively.RESULTS:Compared with the T2DM group,the glycosylated hemoglobin A1c,insulin,glucose tolerance,the homeostasis model assessment of insulin resistance,tumor necrosis factor-α,interleukin 1β,interleukin 16,malondialdehyde,blood lipid,alanine aminotransferase,and aspartate aminotransferase were significantly elevated in the internal dampness group.Their levels were significantly elevated in the Northwest dryness group than in the T2DM and internal dampness groups.The superoxide dismutase,glutathione peroxidase,liver glycogen,and organ-to-weight ratio were significantly declined in the internal dampness group and the Northwest dryness group than in the T2DM group.However,these levels were elevated in the Northwest dryness group than in the internal dampness group.Moreover,the mRNA expression levels of interferon regulatory factor 5 and NF-κB p65,and the protein expression levels of TLR4,MyD88,and NF-κB were significantly higher in the internal dampness and the Northwest dryness groups than the T2DM group.Additionally,the mRNA and protein levels were significantly higher in the Northwest dryness group than in the internal dampness group.CONCLUSION:Northwest dryness syndrome-mediated TLR4/MyD88/NF-κB pathway and chronic inflammation might be associated with the occurrence and development of T2DM.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Electroacupuncture inhibits hippocampal oxidative stress and autophagy in sleep-deprived rats through the protein kinase B and mechanistic target of rapamycin signaling pathway

    ZHENG PengMENG YingLIU MeijunYU Di...
    974-980页
    查看更多>>摘要：OBJECTIVE:To investigate the effects of acupuncture on learning and memory impairment,oxidative stress and autophagy induced by sleep depriv ation in rats,and to analyze the related mechanism.METHODS:Thirty Wistar rats were randomly divided into a normal group,sleep deprivation group and acupuncture group.The rat model of sleep deprivation was established by a modified multiplatform sleep deprivation method.The Baihui(GV20),Shenmen(HT7)and Sanyinjiao(SP6)acupoints of rats were located to give electroacupuncture(density wave,frequency 20 Hz,intensity 1 mA)to maintain the needle feeling,and to keep the needle for 15 min and continuous acupuncture for 7 d.The spatial learning and memory abilities of the rats were detected by the water maze test.The content of malondialdehyde(MDA)and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GPX)in the brain were detected by an assay kit,and the autophagy related proteins light chain 3 alpha(LC3A),light chain 3 beta(LC3B)and Beclin 1 and the activation of the protein kinase B(PKB/AKT)and mechanistic target of rapamycin(mTOR)signaling pathway in the rat's brain were detected by Western blotting.RESULTS:Compared with the normal group,the time spent in the target quadrant(P＜0.05)and the number of times entering the target quadrant(P＜0.05)in the rats of sleep deprivation group were significantly reduced,and the content of MDA was significantly increased(P＜0.01),while the activities of SOD and GPX(P＜0.01)in the brain were significantly decreased,and LC3A Ⅱ/Ⅰ,LC3B Ⅱ/Ⅰand Beclin 1 increased significantly(P＜0.01),while p-AKT(ser473)/AKT,p-mTOR(ser2448)/mTOR and p-p70s6K(thr389)/p70S6 decreased significantly(P＜0.01).Compared with the sleep deprivation group,the time spent in the target quadrant and the times of entering the target quadrant(P＜0.05)in the rats of acupuncture group after 7 d of treatment were significantly increased,Additionally,the content of MDA was significantly decreased(P＜0.05),while the activities of SOD and GPX(P＜0.05)in the brain were significantly increased.Moreover,the levels of LC3A Ⅱ/Ⅰ,LC3B Ⅱ/Ⅰ and Beclin 1 decreased significantly(P＜0.05),and that of p-AKT(ser473)/AKT,p-mTOR(ser2448)/mTOR and p-p70s6K(thr389)/p70s6k increased significantly(P＜0.05).CONCLUSION:Acupuncture can significantly improve the learning and memory damage caused by sleep deprivation and inhibit oxidative stress and autophagy,and its effect is related to the activation of AKT/mTOR signaling.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Regulatory role of electroacupuncture on satellite glial cell activity in the colon and dorsal root ganglion of rats with irritable bowel syndrome

    ZHANG FangYAN CuinaWENG ZhijunWU Luyi...
    981-990页
    查看更多>>摘要：OBJECTIVE:To investigate the role of satellite glial cells in irritable bowel syndrome(IBS)and the effect of electroacupuncture(EA)at the Tianshu(ST25)and Shangjuxu(ST37)combination.METHODS:A model for visceral hypersensitivity in IBS was induced through colorectal distension(CRD)stimulation.Clean-grade male Sprague-Dawley(SD)rats were randomly divided into four groups:a normal group(NG),a model group(MG),an electroacupuncture group(EA),and a glial cell inhibitor group(FCA).Bilateral EA(2/100 Hz,1 mA,30 min)was administered at the Tianshu(ST25)and Shangjuxu(ST37)in week 6.Abdominal withdrawal reflex(AWR)scores were used to assess the behavioral response associated with visceral hyperalgesia,while hematoxylin-eosin staining was employed to evaluate pathological changes in the colon.The protein and mRNA levels of glial fibrillary acidic protein(GFAP)in the colon and colon-related dorsal root ganglion(DRG)were analyzed using immun-ofluorescence,immun-ohistochemistry,Western blotting,real-time polymerase chain reaction.The impact of EA on electrophysiological properties of colon-related DRG neurons was observed through whole-cell patch clamp analysis.RESULTS:EA significantly reduced the visceral pain behavior scores in rats with IBS in response to graded(20,40,60,80 mm Hg)CRD stimulation.Additionally,EA downregulated the protein and mRNA expression levels of GFAP in the colon and colon-related DRG of rats with IBS.EA also regulated the resting membrane potential,rheobase and action potential of colon-related DRG neurons in rats with IBS.CONCLUSIONS:EA can regulate the excitatory properties of colon-related DRG neurons by downregulating the protein and mRNA expression of GFAP in the colon and colon-related DRG,indicating a potential neurobiological mechanism by which EA relieves visceral hypersensitivity in rats with IBS.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Network-based pharmacology and experimental validation to explore the mechanism of action of the Jiawei Pentongling formula(加味盆痛灵方)for the treatment of endometriosis-related pain

    YU SiyunZHANG ShiwenXIA YuLIU Xiaoqing...
    991-999页
    查看更多>>摘要：OBJECTIVE:To investigate the effects and mechanisms of the Jiawei Pentongling formula(加味盆痛灵方,JWPTL)in treating endometriosis-related pain using network pharmacology study and experimental validation.METHODS:Active ingredients and relevant targets of JWPTL,as well as genes for endometriosis-related pain,were collected from public databases.Prediction of core targets and pathways of JWPTL against pain associated with endometriosis by protein-protein interaction(PPI)network work,gene ontology(GO),and Kyoto Encyclopedia of Genes and Genomes(KEGG)functional enrichment analysis.The Sprague-Dawley rat endo-metriosis model was constructed using the autologous endosomal transplantation method,and the successfully modeled rats were randomly divided into the model group and the JWPTL group,with 8 rats in each group.Another 8 rats were set up in the sham group.Rats in the JWPTL group used the rectal delivery method with the addition of JWPTL(7.77 g·kg-1·d-1)once a day for 28 d.Rats in the model and sham-operated groups were given equal amounts of saline using the same administration method.The 50％paw withdrawal threshold(PWT)of the rats was measured at different time points.After the intervention,the expression of phosphatidylinositol 3-kinase(PI3K)and protein kinase B(Akt)proteins and mRNA in endometriotic tissues was detected by immune-ohistochemistry and reverse transcription-polymerase chain reaction.RESULTS:From GeneCards,Online Mendelian Inheritance in Man and other databases,a total of 964 endometriosis(EMs)-related pain targets were screened,142 active ingredients of JWPTL,605 targets,and 221 potential targets were obtained by intersection of Venn diagram;44 core targets were identified by constructing PPI network.KEGG enrichment analysis showed that JWPTL mainly involves the PI3K-Akt signaling pathway,estrogen signaling pathway,hypoxia inducible factor-1 signaling pathway,tumour necrotizing factor signaling pathway,and other signaling pathways in the treatment of EMs-related pain.Animal experiments showed that JWPTL could up-regulate the mechanical pain threshold and reduce the expression of P13K and Akt proteins and mRNA in ectopic endometrial tissues of model rats.CONCLUSIONS:The present study preliminarily analyzed the pharmacological mechanism of the formula,and molecular docking and animal experiments showed the feasibility of this study,suggesting that the formula may inhibit the release of inflammatory factors and reverse the pain associated with EMs by downregulating the PI3K/Akt signaling pathway.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Clinical value of modified Shenling Baizhu powder(加味参苓白术散)in treating targeted therapy-induced diarrhea in non-small cell lung cancer

    WANG MingZHENG Yun
    1000-1005页
    查看更多>>摘要：OBJECTIVE:To assess clinical value of modified Shenling Baizhu powder(加味参苓白术散,SBP)in intervening targeted therapy-induced diarrhea.METHODS:This study was a prospective randomized controlled study.Eighty-five non-small cell lung cancer(NSCLC)patients with diarrhea who took targeted drugs were randomly divided into two groups.The experimental group received modified SBP,while the control group received imodium.During 2 courses of treatment(1 week/course)and 2 weeks of follow-up,we observed remission and recurrence of diarrhea,as well as the improvement of Karnofsky score(KPS)in the two groups and drug safety.RESULTS:Eighty cases were completed,with 40 cases in the experimental group and 40 cases in the control group.The control group's diarrhea remission rate was significantly lower than the experimental group's(P＜0.05).After 2 courses of treatment,the symptom scores of both groups were lower than before,with that of the experimental group remarkably lower(P＜0.05).Furthermore,the experimental group experienced less abdominal fullness and appetite loss than the control group(P＜0.05).There was no prominent difference in overall diarrhea recurrence,time,or KPS after treatment between the two groups(P＞0.05).No unique adverse events occurred in experimental group or control group.CONCLUSION:The modified SBP could improve targeted therapy-induced diarrhea in NSCLC,and is superior to imodium in relieving diarrhea,improving related symptom scores and symptoms,with no obvious drug-related adverse events.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    A pilot study of precision treatment for patients with lung cancer pain by Longteng Tongluo recipe(龙藤通络方)using serum genomics

    WU RuixinFANG QingliangGUAN SisiWEI Xianglong...
    1006-1016页
    查看更多>>摘要：OBJECTIVE:To investigate the efficacy of Longteng Tongluo recipe(龙藤通络方,LTTL)combined with three-step analgesia for the treatment of lung cancer pain,and the changes in serum miRNA expressions before-and after treatment with LTTL and its correlation with lung cancer pain.The possible mechanism underlying LTTL effects on the treatment of lung cancer pain was conducted.METHODS:The pilot study was conducted at the oncology ward of the Yueyang Hospital and the Longhua Hospital between March 2018 and October 2019.A prospective,single-blind,placebo controlled,randomized clinical trial of LTTL or placebo combined with three-step analgesia treatments were administered to 24 cancer pain patients diagnosed with lung cancer.Analgesic efficacy was investigated as the primary outcome.Equivalent morphine consumption and numerical rating scale(NRS)scores were used as the secondary outcome.In the present study,we utilized deep sequencing techniques to compare the differential miRNA expressions in serum samples obtained from two groups:the lung cancer pain treatment group(LTTL+three-step analgesia)and the control group(placebo+three-step analgesia).Next,we employed the target prediction database to investigate the target genes for differential miRNA expressions and Gene Ontology(GO)analysis along with Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis to examine the roles and the major biochemical and signaling pathways related to the differentially expressed target genes,respectively.RESULTS:LTTL treatment significantly reduces the NRS score(P=0.021)as compared to those before treatment,along with significant reductions in the total morphine equivalent consumption(P=0.007)and the average daily equivalent morphine consumption(P=0.003)as opposed to the control group.The expressions of 31 miRNAs differed considerably between the two groups of patients(≥ 2 times up-modulated or down-regulated between these groups,P＜0.05).For instance,the miRNAs expression levels for patients before treatment(has-miR-2110 and has-miR-7d-3p)were significantly enhanced as compared to the healthy people,after LTTL treatment,the expressions of miR-2110 and miR-7d-3p in patients with lung cancer pain reduced significantly.Studies show that the above two miRNAs were significantly associated with lung cancer pain,which could mediate lung cancer pain.Furthermore,we identified 355 genes as potential targets of the 31 differentially expressed miRNAs.Pathway enrichment analyses using KEGG and GO analysis indicated that these target genes may play a crucial role in the development and modulation of lung cancer pain.CONCLUSION:LTTL demonstrated a discernible impact on alleviating lung cancer pain and its mechanism of action may be related to the downregulation of has-miR-2110 and has-miR-7d-3p expressions.This pilot study provides support for further exploration of LTTL in patients with lung cancer pain.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Relative exchangeable copper,a high-quality biomarker for differentiation of Traditional Chinese Medicine syndrome in Wilson's disease

    DING YufengYANG WenmingCHENG YuanYANG Yulong...
    1017-1023页
    查看更多>>摘要：OBJECTIVE:To investigate the value of relative exchangeable copper(REC)in diagnosing Wilson's disease(WD)and to determine its significance in the differentiation of Traditional Chinese Medicine(TCM)syndrome.METHODS:A total of 78 patients with WD were recruited on the same day of the medical visit,and among them,32 were suffering from non-WD(N-WD)and 37 were heterozygous ATP7B carriers(HC)enrolled as controls.Molecular genetic testing was performed for diagnosing WD and HC.Additionally,REC levels in different TCM syndromes were investigated.The correlation between REC and serum ceruloplasmin(Cp),serum copper oxidase(CO),and 24-h urinary copper was analyzed using the Global Assessment Scale and Unified Wilson's Disease Rating Scale and the significance of REC in WD diagnosis was investigated.Resting-state functional magnetic resonance imaging was used to assess the clinical symptoms of WD and analyze its severity in different TCM syndromes.RESULTS:REC determination helped in significantly distinguishing patients with N-WD or HC from those with WD with a cut-off of 21.15％.Furthermore,the comparative analysis of REC ratios among different TCM syndromes showed markedly high REC levels in the dampness-heat internal accumulation syndrome group.Additionally,the seed-based functional connectivity value from the putamen to the cerebellum was significantly correlated with TCM syndromes.CONCLUSION:REC level is a high-quality biomarker for diagnosing WD that effectively reflects disease severity and plays an essential role in deciding on treatment strategies and prognosis.Furthermore,REC levels are closely related to TCM syndromes in WD;thus,it is a potential objective quantitative indicator for distinguishing TCM syndromes in WD.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Association of miR-499 rs3746444,miR-149 rs2292832 polymorphisms and their expression levels with helicobacter pylori-related gastric diseases and Traditional Chinese Medicine syndromes

    LIU QiYU ChangYE JintongZHANG Ling...
    1024-1034页
    查看更多>>摘要：OBJECTIVE:To provide an objective experimental basis for the gastric mucosa pathological evolution and the transformation of different Traditional Chinese Medicine(TCM)syndromes in helicobacter pylori(H.pylori)-related gastric diseases(HPGD)patients,based on the combination of TCM syndrome differentiation,molecular biology and histopathology.METHODS:A total of 203 participants were enrolled in this study.The expressions of miR-499/miR-149 and H.pylori infection in the gastric tissues from all participants were detected.The genotyping for miR-499 rs3746444 and miR-149 rs2292832 was performed.RESULTS:In H.pylori positive subjects,the proportion of precancerous gastric lesions(PGL)in liver-stomach disharmony syndrome(LSDS)group was higher than in spleen Qi deficiency syndrome(SQDS)group(P＜0.001);The proportion of gastric cancer(GC)in SQDS group was higher than in spleen-stomach damp-heat syndrome(SSDHS)group and LSDS group(all P＜0.001).We also found C allele of miR-149 rs2292832 was linked to lower risk of gastric atrophy[miR-149 rs2292832 C vs T:adjusted odds ratio=0.207;95％confidence interval(0.043-0.989);P=0.048].Compared with healthy control(HC)group,the expression of miR-499 was significantly increased in GC group,while the expression of miR-149 was significantly decreased in chronic inflammation group,PGL group and GC group(all P＜0.05).Test for trend showed that GC risk was on a rising trend with the increasing expression of miR-499 and decreasing expression of miR-149(both P for trend＜0.05).CONCLUSION:The C allele of miR-149 rs2292832 may be a protective factor for gastric mucosal atrophy.H.pylori may participate in the evolution of benign to malignant gastric mucosa lesions by inducing the overexpression of miR-499 and down regulation of miR-149.In addition,patients with H.pylori infection combined SQDS or LSDS may have higher risk of gastric mucosal malignant lesions.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    International standard of Traditional Chinese Medicine techniques:the operating specification for Traditional Chinese Medicine enema(2022)

    YU MiaoPEI Xiaohua
    1035-1043页
    查看更多>>摘要：Chinese Medicine Enema is one of the external therapies in Traditional Chinese Medicine(TCM),which has been widely used clinically.The"International Standard of Traditional Chinese Medicine Techniques:The Operating Specifications for Chinese Medicine Enema is drawn up by the Beijing Hospital of Traditional Chinese Medicine affiliated to Capital Medical University and Beijing University of Chinese Medicine Xiamen Hospital in collaboration with domestic TCM universities and hospitals.The specification includes definition,operating process,points for attention and contraindications.It is targeted to provide reference for TCM providers at home and abroad with TCM background in clinical decision-making.
      原文链接:
    • NETL
    • NSTL
    • 万方数据

    Development of international guidelines by Tuina practitioners for specific acupoints of paediatrics Tuina(2022 version)

    LAN XiaoxueSUN YananWENG ZhiwenWANG Yue...
    1044-1051页
    查看更多>>摘要：OBJECTIVE:To establish a standardized framework encompassing the precise locations,manipulations,functions and indications of specific acupoints in the field of paediatric Tuina.METHODS:The development of consensus involved three distinct stages.Initially,a list of paediatric Tuina specific acupoints was compiled based on an extensive literature review,which was subsequently supplemented through expert interviews.In the second stage,the Delphi method was employed to assess the significance of acupoint locations,manipulations,functions,and indications.In situations where the questionnaire survey failed to yield agreement or when the experts held reservations,the nominal group approach was utilized during the expert consensus meeting.The final version of the technical standardized material was ultimately determined during an expert consensus conference.After undergoing external peer review and evaluation,the completed draft was prepared for public disseminationRESULTS:The comprehensive list identified a total of 66 specific acupoints.The location and manipulation questionnaire consisted of 156 items based on the literature database,while the function and indication questionnaire contained 116 items.Two rounds of Delphi surveys were conducted for the location and manipulation category,and another two rounds of Delphi surveys were conducted for the function and indication category.During the experts consensus meeting The panel of experts conducted in-depth discussions on 61 questions,resulting in the formulation of technical guidelines for the locations,manipulations,functions,and indications of 64 paediatric Tuina acupoints.Subsequently,the research team compiled and edited the draft of the technical guidelines for acupoints of paediatric Tuina,which was finalized after external review and feedback.CONCLUSION:This study successfully established the recognized technical standards for practitioners of paediatric Tuina,thereby standardizing clinical practices and providing a foundation setting the framework for future research.The guidelines offer theoretical insights and recommendations for conducting clinical studies comparing different acupoint sites,as well as modifying or enhancing treatment regimens.
      原文链接:
    • NETL
    • NSTL
    • 万方数据