摘要
目的:探讨饲料中添加根皮素对热应激环境下肉鸡腿肌肉品质及其抗氧化性能的影响.方法:选取 160 只体质量相近的 22 日龄AA肉鸡,随机分为NT、HT、LT和PT等 4 组,每组 4 个重复,每个重复10 只.其中,NT为对照组,饲养温度为(23.0±0.61)℃,相对湿度为 50.8%±5.41%;HT、LT、PT为热应激组,饲养温度为(30.5±0.69)℃,相对湿度为 89.7%±6.70%,NT和HT饲喂基础日粮,LT、PT分别饲喂在基础日粮中添加 100、200 mg/kg根皮素的日粮.在肉鸡 42 日龄时每个重复随机挑选 3 只肉鸡,屠宰后进行腿肌肉品质及抗氧化性能的测定.结果:与HT相比,NT肉鸡腿肌亮度、滴水损失、失水率、蒸煮损失、MDA水平均显著下降(P<0.05),CAT、GSH、GSH-Px、T-AOC水平均显著上升(P<0.05).与HT相比,PT肉鸡腿肌亮度、肌滴水损失、失水率、蒸煮损失、MDA水平均显著下降(P<0.05),CAT、GSH、GSH-Px、T-AOC水平均显著上升(P<0.05).与HT相比,LT的T-AOC水平显著上升(P<0.05).结论:饲料中添加根皮素可有效改善热应激对肉鸡腿肌肉品质及抗氧化性能的影响,且以添加 200 mg/kg效果较好.
Abstract
Objective:The effects of phloretin as a feed additive on muscle quality and antioxidant properties of broilers under heat stress were investigated.Methods:A total of 16022-day-old AA broilers with similar body weight were randomly divided into NT,HT,LT and PT groups with 4 replicates per group and 10 broilers per replicate.NT was the control group,the feeding temperature was(23.0±0.61)℃,and the humidity was 50.8%±5.41%.HT,LT and PT were heat stress groups.The feeding temperature was(30.5±0.69)℃ and the humidity was 89.7%±6.70%.NT and HT were fed with basal diet.LT was fed with basal diet supplemented with 100 mg/kg phloretin,and PT was fed with basal diet supplemented with 200 mg/kg phloretin.At 42 days of age,3 broilers were randomly selected from each replicate to detect leg muscle quality and make leg muscle tissue homogenate,which was stored at-70℃,and the kit was used to detect the antioxidant properties of leg muscle.Results:Compared with the HT,the leg muscle brightness,muscle drip loss,water loss rate,cooking loss,and MDA levels in NT were significantly decreased(P<0.05),and CAT,GSH,GSH-Px,and T-AOC levels were significantly increased(P<0.05).Compared with the HT,the leg muscle brightness,muscle drip loss,water loss rate,cooking loss,and MDA levels in PT were significantly decreased(P<0.05),and CAT,GSH,GSH-Px,and T-AOC levels were significantly increased(P<0.05).Compared with HT,the T-AOC level of the LT group was significantly increased(P<0.05).Conclusion:Adding 200 mg/kg phloretin to the feed can effectively reduce the effect of heat stress on leg muscle quality and antioxidant performance of broilers,and improve the resistance of broilers to heat stress.
基金项目
国家自然科学基金(32002223)
安徽省自然科学基金面上项目(2108085MC113)
安徽省高校自然科学研究重点项目(KJ2020A0084)
国家十四五重点研发计划项目(2022YFD1601905)
维普
万方数据