Objective:To study the effects of foliar spraying sodium selenite on the yield,quality,selenium and related trace element content of pepper,analyze the accumulation and tolerance characteristics of pepper to sodium selenite,and select the best concentration of sodium selenite for pepper growth.Methods:The substrate cultivation method was used in this study.Bianjiao No.1 pepper variety was taken as the research object.Five concentrations of sodium selenite were set,which were 0,50,100,150,200 mg/L,respectively.The amount of sodium selenite sprayed on pepper in each treatment was 50 mL,and three replicates were set in each treatment.After 60 days of cultivation,the yield,quality,selenium and related trace elements of pepper were determined and analyzed.Results:The application of sodium selenite showed a dose effect of"low concentration promoting,high concentration inhibiting"on pepper yield.When the concentration of sodium selenite was 100 mg/L,the yield increased by 29%compared with CK.When the concentration of sodium selenite was 150 mg/L,the yield of pepper decreased by 22.05%compared with CK.When the concentration of sodium selenite was in the range of 0-200 mg/L,the selenium content of pepper showed a gradual upward trend,with significant difference;The percentage of organic selenium in total selenium in pepper fruits increased first and then decreased with the increase of sodium selenite concentration.For each experimental treatment,when the concentration of sodium selenite gradually increased,the content of calcium,iron and copper in pepper fruits increased,as well as crude protein,vitamin C,total sugar and other quality indicators,but the content of magnesium and zinc had no significant difference.were rising.Conclusion:Spraying the appropriate concentration of sedium selenite on the leaves could significantly increase the yield and the contents of selenium,calcium and iron in the fruits,and effectively improve the quality of pepper.The optimum concentration of sedium selenite is 50-100 mg/L.Therefore,the rational use of sedium selenite is one of the important directions in the development of Se-enriched agriculture,it is also an important link to promote high-yield and efficient production of crops.