Objective:In order to provide technical basis for more effective prevention and control of cotton Fusarium wilt and the development of new biological pesticides,this study explored the inhibitory activity of metabolites of biocontrol bacteria and chemical fungicides against Fusarium oxysporum f.sp.vasinfectum.Methods:The inhibition characteristics of biocontrol bacteria,fermentation supernatant,lipopeptide crude extract and chemical fungicides on cotton Fusarium wilt were determined by confrontation culture method and mycelial growth rate method.Results:The inhibition rate of biocontrol strain SJ06 against cotton Fusarium wilt was 78.24%by confrontation culture.When the concentration of fermentation supernatant increased from 6.25 μL/mL to 100.00 μL/mL,the inhibition rate increased from 46.90%to 80.30%,and the EC50 was 7.37μL/mL.The inhibition rate increased from 19.90%to 77.30%when the concentration of lipopeptide crude extract increased from 673.96μg/mL to 8120.00μg/mL,and the EC50 of lipopeptide crude extract to pathogen was 2720.20 μg/mL.Cotton Fusarium wilt was highly sensitive to carbendazim and tebuconazole.The EC50 to the pathogen was 0.34μg/mL and 0.49 μg/mL,respectively.The EC50 of myclobutanil,difenoconazole and propiconazole against pathogens were 1.07,1.34,1.54 μg/mL,respectively.The inhibitory activity of propineb against bacteria was poor,the EC50 was 63.25 μg/mL.Conclusion:Bacillus amyloliquefaciens fermentation metabolites and ergosterol biosynthesis inhibitors such as tebuconazole have good inhibitory activity against Fusarium oxysporum f.sp.vasinfectum.
关键词
棉花枯萎病/生防菌代谢产物/化学杀菌剂
Key words
Cotton Fusarium wilt/Metabolites of biocontrol bacteria/Chemical fungicides
维普
万方数据