Objective:In order to optimize the extraction process of total flavonoids in sea-buckthorn and clarify its antioxidant property,sea-buckthorn was selected as the research object in this experiment.Methods:The yield of sea-buckthorn flavonoids was used as the index value,and ethanol solution was used as the extractant and combined with ultrasonic-assisted extraction technology,single factor test and response surface method were carried out on the four factors of extraction time,extraction temperature,ethanol concentration and solid-liquid ratio,and the antioxidant property of sea-buckthorn flavonoids were studied.Results:The optimum extraction conditions were as follows:extraction time 23 min,extraction temperature 80℃ ethanol concentration 50%,solid-liquid ratio 1∶20 g/mL.Under the conditions,the yield of sea-buckthorn flavonoids was the highest,which was 2.316 mg/g.The effects of various factors on the yield of flavonoids were solid-liquid ratio,ethanol concentration,extraction time and extraction temperature.The antioxidant results showed that sea-buckthorn flavonoids had excellent scavenging effects on DPPH free radicals and hydroxyl radicals.When the concentration of sea-buckthorn flavonoids reached 5 mg/mL,the scavenging rate of DPPH free radical reached 87.5%,and the scavenging rate of hydroxyl radicals reached 77.7%.Conclusion:Sea-buckthorn is rich in flavonoids,and under certain concentration conditions,sea-buckthorn flavonoids have good antioxidant properties.
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