摘要
目的:探究褪黑素对卵巢颗粒细胞发育相关基因表达的影响.方法:不同浓度的褪黑素(0、10-3、10-5、10-7、10-9、10-11 mol/L)分别处理颗粒细胞24、48、72 h后提取细胞总RNA,采用Real-time PCR检测抗氧化(GPX4、SOD1)、发育(EGFR、DNMT1a、FSHR)和TGFβ家族(BMP6)相关基因的表达.结果:不同浓度褪黑素处理24、48、72 h对抗氧化(GPX4、SOD1)、发育(EGFR、DNMT1a、FSHR)和TGFβ家族(BMP6)相关基因的表达均有促进或抑制作用(P<0.05),高浓度褪黑素(10-3 mol/L)在48 h对G PX 4、SOD1的促进效果最显著(P<0.05);高浓度褪黑素(10-3 mol/L)不影响EG FR、DNMT1a的表达,其他浓度褪黑素均促进EGFR、DNMT1a的表达(P<0.05);低浓度褪黑素(10-11 mol/L)显著抑制FSHR的表达(P<0.05);褪黑素对于BMP6的表达在24 h为促进作用(P<0.05),48 h时抑制其表达(P<0.05),72 h时高浓度褪黑素(10-3 mol/L)和低浓度褪黑素(10-11 mol/L)无显著作用,其他浓度为促进作用(P<0.05).结论:褪黑素可以通过促进抗氧化相关基因(GPX4、SOD1)、发育相关基因(EGFR、DNMT1a)和TGFβ家族(BMP6)相关基因的表达,抑制FSHR的表达来保护颗粒细胞,抑制其凋亡.
Abstract
Objective:To investigate the effect of melatonin on the expression of development-related genes in ovarian granulosa cells.Methods:Total cellular RNA was extracted after treating granulosa cells with different concentrations of melatonin (0 ,10-3 ,10-5 ,10-7 ,10-9 ,and 10-11 mol/L) for 24 , 48 , 72 h , respectively , and the expression of genes related to antioxidant ( GPX4 , SOD1 ) , developmental (EGFR ,DNMT1a ,and FSHR) ,and TGFβ family (BMP6) was detected by real-time PCR.Results:Different concentrations of melatonin promoted or inhibited the expression of antioxidant (GPX4 , SOD1) ,developmental (EGFR , DNMT1a , FSHR) ,and TGFβ family (BMP6)-related genes at 24 ,48 ,72 h (P<0.05) ,and a high concentration of melatonin (10-3 mol/L) had the most significant (P<0.05) promotional effect on GPX4 ,SOD1 ,and SOD1 at 48 h.The high concentration of melatonin (10-3 mol/L) had the most significant (P<0.05) promotional effect on GPX4 and SOD1 at 48 h (P<0.05).SOD1 at 48 h.High concentration melatonin (10-3 mol/L) had the most significant effect on GPX4 and SOD1 (P<0.05).High concentration melatonin (10-3 mol/L) did not affect the expression of EGFR and DNMT1a ,and all other concentrations of melatonin promoted the expression of EGFR and DNMT1a (P< 0.05) ,and low concentration melatonin (10-11 mol/L) significantly inhibited the expression of FSHR (P<0.05) ,and the effect of melatonin on BMP6 expression at 24 h was promotional (P<0.05).Expression of BMP6 at 24 h (P<0.05) and inhibited its expression at 48 h (P<0.05).At 72 h ,the high concentration of melatonin (10-3 mol/L) and the low concentration of melatonin (10 -11 mol/L ) did not have any significant effect , and the other concentrations had a promotional effect (P < 0.05).Conclusion:Melatonin can protect granulosa cells and inhibit their apoptosis by promoting the expression of antioxidant-related genes (GPX4 , SOD1 ) , development-related genes (EGFR , DNMT1a) , and TGFβ family (BMP6)-related genes , and inhibiting the expression of FSHR.
基金项目
安徽省自然科学基金面上项目(2008085MC94)
安徽省高校自然科学研究项目(KJ2021A0869)
安徽省重点研究与开发计划项目(202204c06020076)
维普
万方数据