纤维水平对皖西白鹅血液生理生化指标、血清细胞因子及胸腺组织结构的影响

Effects of fiber level on blood physiological and biochemical indexes,serum cytokines and thymus microstructure of Wanxi white geese

张康 ¹李蕾蕾 ¹张鑫 ¹方素庭 ¹唐军 ¹任曼 ²胡倩倩 ²李小金 ²李升和²

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作者信息

1. 安徽科技学院动物科学学院,安徽凤阳 233100
2. 安徽科技学院动物科学学院,安徽凤阳 233100;动物营养调控与健康安徽省重点实验室,安徽凤阳 233100
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摘要

目的:研究纤维水平对皖西白鹅血液生理生化指标、血清细胞因子及胸腺组织结构的影响,探究皖西白鹅饲粮适宜的纤维添加量.方法:选择200只皖西白鹅进行试验,随机分为3％、5％、7％、9％、11％纤维水平组,每组5个重复.结果:28日龄7％、9％和11％纤维组WBC极显著高于5％纤维组(P＜0.01),7％、9％纤维组WBC极显著高于3％组(P＜0.01).28日龄皖西白鹅9％和11％纤维组CRE-E极显著高于3％和5％组(P＜0.01),11％纤维组CRE-E显著高于7％纤维组(P＜0.05),7％组CRE-E显著高于3％组(P＜0.05).14日龄皖西白鹅3％纤维组IL-10和IFN-γ极显著高于其他试验组(P＜0.01);28日龄皖西白鹅3％纤维组IL-10、IFN-γ水平极显著高于其他试验组(P＜0.01),3％纤维组IL-6显著高于9％组(P＜0.05).28日龄皖西白鹅5％、7％皮质面积显著高于3％、11％组(P＜0.05),7％纤维组皮髓比极显著高于3％组(P＜0.01),5％组皮髓比显著高于3％组(P＜0.05).结论:饲粮中添加7％、9％纤维水平可提高皖西白鹅白细胞、红细胞等细胞水平,提高CRE-E、TG、TC、HDL、LDL等与氨基酸和脂类代谢相关指标,降低IL-10、IFN-γ、IL-6等炎症反应相关因子水平,可促进胸腺发育.本试验条件下,以免疫指标为判断依据,育雏期皖西白鹅饲粮纤维水平为6.83％～8.66％为宜.

Abstract

Objective:To study the effects of fiber level on blood physiological and biochemical indexes,serum cytokines and thymus microstructure of Wanxi white geese,for exploring the appropriate dietary fiber supplemental level for Wanxi white geese.Methods:200 Wanxi white geese were randomly divided into 3％,5％,7％,9％and 11％fiber level groups with 5 replicates per group.Results:In 28 days of age,WBC in 7％,9％and 11％fiber groups was significantly higher than that in 5％fiber group(P＜0.01).WBC in 7％and 9％fiber groups was significantly higher than that in 3％group(P＜0.01).In 28 days of age,CRE-E in 9％and 11％fiber groups was significantly higher than that in 3％and 5％groups(P＜0.01).CRE-E in 11％fiber group was remarkably higher than that in 7％fiber group(P＜0.05).CRE-E in 7％group was remarkably higher than that in 3％group(P＜0.05).In 14 days of age,the levels of IL-10 and IFN-γ in 3％fiber group were significantly higher than those in other experimental groups(P＜0.01).In 28 days of age,the levels of IL-10 and IFN-γ in 3％fiber group were significantly higher than those in other experimental groups(P＜0.01),and IL-6 in 3％fiber group was remarkably higher than that in 9％group(P＜0.05).In 28 days of age the cortical area of 5％and 7％of Wanxi white geese was remarkably higher than that of 3％and 11％groups(P＜0.05),the Corticomedullary ratio of 7％fiber group was significantly higher than that of 3％group(P＜0.01),and the skin-pulp ratio of 5％group was remarkably higher than that of 3％group(P＜0.05).Conclusion:The dietary supplementation of 7％and 9％fiber has been shown to enhance the levels of white blood cells and red blood cells,as well as increase the concentrations of CRE-E,TG,TC,HDL,LDL,which are relevant markers associated with amino acid and lipid metabolism.Moreover,it effectively reduces the levels of IL-10,IFN-γ,IL-6,which are inflammatory response-related factors while promoting thymus development in Wanxi white geese.Based on immune indicators observed in this experiment,a dietary fiber level ranging from 6.83％to 8.66％is recommended for Wanxi white geese during their brooding period

关键词

皖西白鹅/纤维水平/血液/细胞因子/胸腺/组织结构

Key words

Wanxi white geese/Fiber level/Blood/Cytokines/Thymus/Microstructure

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基金项目

国家自然科学基金(31672502)

国家自然科学基金(31502137)

国家自然科学基金(31501968)

安徽省科技重大专项(17030701004)

安徽省高等学校学科(专业)拔尖人才学术项目(gxbjZD2020080)

安徽省高等学校优秀科研创新团队项目(2022AH010088)

安徽省高等学校优秀青年科研项目(2022AH030146)

安徽省地方鹅种基因库项目()

安徽省滁州市科技计划(2022ZN002)

出版年

2024

安徽科技学院学报

安徽科技学院

安徽科技学院学报

影响因子：0.434

ISSN：1673-8772

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