Objective:To establish an efficient and stable regeneration in vitro of red long eggplant(Solanum melongena L.)to provide materials and technology for genetic transformation,gene function verification,and mutation breeding in vitro.Methods:Cotyledons,euphyllas,hypocotyls,and stem from aseptic sprouting seedlings of variety Wanqie15 were inoculated into MS containing 15 hormone combination for screening suitable explants.Subsequently,the hormone ratio in euphyllas callus induction,redifferentiation and shoots rooting were studied.Results:Euphyllas was the suitable explant,and the inducing differentiation activity of 6-BA was very weak,and it was easy for IAA to induce rooting,while NAA has a better effect on callus induction.MS+0.1 mg/L TDZ+0.2 mg/L NAA was suitable for euphyllas callus induction,and the induction rate was 100％,the callus grew well.The best medium for callus redifferentiation was MS+0.1 mg/L TDZ,and adventitious bud differentiation rate was 100％,average number of strong differentiation buds was 5.30.The optimum rooting medium was MS with rooting rate reaching 100％,and the average number of roots was 4.60.After domestication and transplantation,the survival rate of regeneration seedling was over 90％.Conclusion:This study established an efficient and stable regeneration system of red long eggplants variety Wanqie15 by using euphyllas as explants via callus induction,redifferentiation,rooting,domestication,and transplantation.
关键词
红线茄/外植体/真叶/愈伤组织/离体再生/茄子
Key words
Red long eggplant/Explant/Euphyllas/Callus/Regeneration in vitro/Eggplant
万方数据