Effects of dehydroepiandrosterone supplementation on indicators of fat deposition,blood lipids,antioxidant capacity and vascular endothelial function in broilers fed with high-fat feed
To evaluate the effects of dehydroepiandrosterone(DHEA)on lipid metabolism,antioxidant activity,and endothelial function in broilers fed with high-fat diet.A total of 200 Cyan-shank Partridge chickens(15-day-old)were allocated randomly into 5 groups.The control group(CON)was fed with basic feed,while the high-fat feed group(HFD),low-dose DHEA intervention group(HFD+D5),medium dose DHEA intervention group(HFD+D15),and high-dose DHEA intervention group(HFD+D45)were fed with high-fat feed supplemented with 0,5,15,45 mg/kg DHEA.In the second and fourth week of the experiment,the intramuscular fat width,subcutaneous fat thickness,sebum mass,and abdominal fat mass of the chickens were measured,and the abdominal fat percentage and sebum percentage were calculated.The blood samples were collected to test hormones and biochemical indicators;and the liver tissue samples were collected to detect antioxidant function and hepatic lipase(HL)activity.Compared with the control group,high-fat feed increased significantly the abdominal fat mass,abdominal fat percentage,sebum percentage,and blood triglycerides(TG),lipoprotein a(LP(a)),angiotensin Ⅱ(Ang Ⅱ)levels of broilers,but reduced serum high-density lipoprotein(HDL),T3,T4,FT3,FT4,and nitric oxide(NO)concentrations.Meanwhile,high-fat feed increased significantly liver malondialdehyde(MDA)contents,but decreased activities of HL,total superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in liver.Compared with the HFD group,DHEA treatment showed a decreasing trend in body fat deposition and serum levels of TG,total cholesterol(TC),and LP(a)in broilers fed with high-fat feed.Meanwhile,DHEA increased serum levels of T3,T4,FT3,FT4,NO,as well as activities of HL,SOD,and GSH-Px in liver,while reducing liver MDA contents and plasma Ang Ⅱ levels.Dietary supplementation of DHEA has a certain alleviating effect on lipid metabolism disorders,oxidative stress,and endothelial dysfunction in broilers induced by high-fat feed.Its effect may accelerate fat breakdown,inhibit fat deposition,weaken oxidative stress,and improve vascular endothelial function by enhancing liver HL activity and regulating serum thyroid hormone levels.
万方数据
维普
