Cloning and characterization of a full cDNA of photosystem I gene encoding the light harvesting chlorophyll a/b-binding protein from Bambusa oldhamii
To study the molecular mechanism of photosynthesis, the BoLhca4-1 (GenBank accession number; EF690303) , a member of the cab gene family, was isolated from Bambusa oldhamii by RT-PCR and RACE. The cDNA of BoLhca4-1 is 931 bp in length,which contains an 735 bp open reading frame (ORF) , encoding a poly-peptide of 244 amino acids with predicted molecular mass of 26. 8 ku. The results of sequence analysis showed that the BoLhca4-1 is a cab binding protein gene of photosystem Ⅰ and its encoding protein is highly homologous to the cab protein from rice with 86.1% identity. In addition, it was found that the Lhca4-1 was more related to the rice cab protein from the Phylogenetic tree. Based on the RT-PCR method, it was identified that BoLhcaAA gene displays a higher expression level in leaf tissue than that in sheath and stem.
Bambusa oldhamiiphotosystem Ⅰlight harvesting chlorophyll a/b-binding protein (cab)gene cloningexpression analysis
国家科技期刊平台
NSTL
万方数据
维普
