Extraction of flavonoids from Phyllostachys pubescens shoot
Flavonoids from Phyllostachys pubescens shoot were extracted by methods of water, ethanol b ackflow and ultrasonic wave extractions. The optimum extraction condition was obtained by single factor analysis and orthogonal test. The results showed that the yield of flavonoids was 0.706％ by water extraction under the conditions of 60℃, 1∶ 30 solid-liquid ratio, pH 9.0 and 1.5 h. The order of the factors which affected water extraction was soild-liquid ratio ＞ extraction temperature ＞ extraction time ＞ pH. The yield of flavonoids was 1.063％ by the method of ethanol backflow under the conditions of 1∶ 40 solid-liquid ratio, 60℃, 70％ ethanol and 2.0 h. The order of the factors which affected the ethanol backflow extraction was soild-liquid ratio ＞ extraction temperature ＞ extraction time ＞ volume fraction of ethanol solution. The yield of flavonoids was 0.797％ by methods of ultrasonic wave under the conditions of 1∶ 40 solid-liquid ratio, 50℃, 70％ ethanol and 30 min. The order of the factors which affected the ultrasonic wave extraction was soild-liquid ratio ＞ extraction temperature ＞ extraction time ＞ volume fraction of ethanol solution.
Phyllostachys pubescens shootflavonoidsorthogonal test
国家科技期刊平台
NSTL
万方数据
维普
