首页|EPG技术分析不同品种茶树抗假眼小绿叶蝉取食行为的差异

EPG技术分析不同品种茶树抗假眼小绿叶蝉取食行为的差异

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假眼小绿叶蝉是我国最严重的茶树害虫,不同品种对其表现出抗性或感性,EPG是近年用于快速检测作物抗刺吸式害虫的新技术.以浙江、江苏主推的6个茶树良种为供试材料,用可视的DC-EPG技术记录了该叶蝉的刺探取食行为.结果表明:(1)叶蝉在6个品种茶树上的7种基本刺吸波形是相同的.(2)在锡荼5号、苏荼1号上的刺探次数多,在韧皮部内的被动取食波-E波的持续时间长;在龙井43上刺探次数少,在韧皮部内的被动取食波E波持续时间短:苏荼120、中茶108和安吉白荼则居中.(3)使用聚类分析、多重比较分析,评判6个品种茶树抗叶蝉取食能力由强至弱的顺序为龙井43>苏茶120>中荼108>安吉白荼>苏茶1号>荼5号,该顺序与田间观察的6个品种茶树上叶蝉虫口密度由小到大的顺序基本一致.认为EPG技术可用于评判不同品种茶树对叶蝉抗性的强弱.
Analysis of the feeding behaviours of tea green leafhoppers on resistant to sensible cultivars of tea plants by electrical penetration graphy techniques
Tea green leafhopper, Empoasca vitis G the, is the most important pest in tea plantation communities in China, and various tea cultivars exhibit resistance or sensibility for the pest. Electrical penetration graphy (EPG) is a new technique that is used to detect the resistance of crops against leafhopper and other pierc ing-sucking pests. During the present work, the 6 elite cultivars, being extended in Zhejiang and Jiangsu Province,were acted as the examining material, and the feeding behaviours of the leafhopper were recorded by the video DC-EPG technique. The results showed as follows: (1)Seven types of primary EPG waveforms emerged on each tested cultivar. (2)There were more probing number and long-drawn E waveform when the stylets passively sucked in phloem on Xicha 5 and Sucha 1 cultivar;, on the contrary, there were less probing number and short E waveform on Longjing 43 cultivar.Moreover, Sucha 120, Zhongcha 108 and Anjibaicha were placed in the middle.(3)Recurring to cluster analysis and multiple range analysis, the resistance of 6 cultivars of tea plants against the leafhopper were judged, and the ranking order from strong to weak were: Longjing 43>Sucha 120> Zhong cha108>Anjibaicha>Sucha 1>Xicha 5, which was in correspondence with the sequence, from little to big, of the population density of the leafhopper on the six tea cultivars in the tea fields. It is considered that EPG technique can be used to judge the degree of the resistance or the sensibility of tea cultivars against the leafhopper.

tea green leafhoppertea plant cultivarpiercing and sucking behaviourvideo DC-EPGcluster analysisresistance

刘丽芳、徐德良、穆丹、韩宝瑜

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中国农业科学院茶叶研究所,杭州,310008

中国计量学院浙江省生物计量及检验检疫技术重点实验室,杭州,310018

无锡市茶叶品种研究所,无锡,214063

假眼小绿叶蝉 茶树品种 刺吸行为 可视DC-EPG 聚类分析 抗虫性

国家自然科学基金浙江省重大科技专项项目浙江省自然科学基金

31017442008C12070Y3100375

2011

安徽农业大学学报
安徽农业大学

安徽农业大学学报

CSTPCDCSCD北大核心
影响因子:0.412
ISSN:1672-352X
年,卷(期):2011.38(2)
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