首页|夏秋茶对中温大曲中主要微生物区系及白酒风味的影响

夏秋茶对中温大曲中主要微生物区系及白酒风味的影响

Effects of summer and autumn tea on major microflora of medium temperature Daqu and flavor of Baijiu

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为探究夏秋茶对酒曲中主要微生物区系和酒体香气物质的影响,在制曲过程中添加不同量的夏秋茶(5%、10%和15%)制备茶型大曲,选择15%茶型大曲并添加夏秋茶酿制茶香型白酒(原酒),设置普通大曲和普通浓香型白酒(原酒)作为对照组.对普通大曲、5%、10%和15%茶型大曲中主要微生物数量和发酵性能进行了对比分析,相较于普通大曲,5%、10%和15%茶型大曲中细菌数量有不同程度减少,霉菌数量有10倍量级的增加,其中15%茶型大曲霉菌数量达到1.9×105 CFU·g-1,5%和10%茶型大曲酵母菌数量均有增加;3种茶型大曲的发酵力和糖化力也有显著增加.通过顶空固相微萃取-气相色谱-质谱联用技术(Headspace solid phase microextractiongas-chromatography mass-spectrometry,HS-SPME-GC-MS)对2种白酒中香气成分进行检测,并结合正交偏最小二乘判别分析(Orthogonal partial least squares discriminant analysis,OPLS-DA)筛选2种白酒的差异香气物质,最终确定了茶香型白酒中的4种差异香气物质,分别是庚酸乙酯、月桂酸乙酯、癸酸乙酯和苯甲酸乙酯.由于夏秋茶的添加对微生物进行选择性培养,使得霉菌和酵母菌分别成为发酵前期、发酵后期的优势菌种,并且代谢利用夏秋茶中的内含成分产生更多的酸类物质,最终形成庚酸乙酯、月桂酸乙酯、癸酸乙酯和苯甲酸乙酯等香气物质.结果为夏秋茶资源的增值利用以及茶香型白酒的开发研究提供依据.
In order to explore the effect of summer and autumn tea on the major microflora in Daqu and aroma substances in Baijiu, different amounts of summer and autumn tea (5%, 10% and 15%) were added during the Daqu-making process to prepare tea-type Daqu, and 15% tea-type Daqu with the addition of summer and autumn tea was selected to make tea-flavor Baijiu (original wine), and ordinary Daqu and ordinary strong-flavor Baijiu (original wine) were set as control groups. Compared with the ordinary Daqu, 5%, 10% and 15% tea-type Daqu, the number of bacteria in 5%, 10% and 15% tea-type macquat decreased to different degrees, and the number of mold increased ten folds, among which, the number of mold in 15% tea-type Daqu reached 1.9×105 CFU·g-1, and the number of yeast in 5% and 10% tea-type Daqu increased. The fermentability and saccharification power of the three tea-type Daqu also increased significantly. The aroma components in the two liquors were detected by Headspace solid phase microex-traction gas-chromatographymass-spectrometry (HS-SPME-GC-MS) combined with orthogonal partial least squares discriminant analysis (OPLS-DA) to screen the differential aroma substances of the two liquors, and finally four dif-ferential aroma substances in the tea-flavor Baijiu were identified, namely ethyl heptanoate, ethyl laurate, ethyl caprate and ethyl benzoate. Due to the selective cultivation of microorganisms by the addition of summer and autumn tea, the mold and yeast became the dominant strains in the pre-fermentation and post-fermentation stages, respectively, and bacteria metabolically utilized the intrinsic components in summer-autumn tea to produce more acids, eventually forming ethyl heptanoate, ethyl laurate, ethyl decanoate and ethyl benzoate aroma substances. The result provides a basis for the value-added utilization of summer and autumn tea resources and the development of tea-flavor Baijiu.

summer and autumn teatea-type Daqutea-flavor Baijiugas chromatography-mass spectrometryethyl heptanoate

肖严严、程伟、陈兴杰、潘天全、薛锡佳、杨金玉、杜先锋

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安徽农业大学茶与食品科技学院 农业农村部江淮农产品精深加工与资源利用重点实验室,安徽省农产品加工工程实验室,合肥 230036

安徽金种子酒业股份有限公司,阜阳 236023

夏秋茶 茶型大曲 茶香型白酒 气相色谱-质谱联用 庚酸乙酯

安徽省科技重大专项

201903a06020025

2024

安徽农业大学学报
安徽农业大学

安徽农业大学学报

CSTPCD
影响因子:0.412
ISSN:1672-352X
年,卷(期):2024.51(1)
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