安徽农业大学学报2024,Vol.51Issue(1) :145-151.DOI:10.13610/j.cnki.1672-352x.20240313.006

降解氨基甲酸乙酯酿酒酵母的筛选及其应用

Screening of ethyl carbamate degraded brewer's yeast and its application

夏尚帆 李奎 王文武 张惠玲
安徽农业大学学报2024,Vol.51Issue(1) :145-151.DOI:10.13610/j.cnki.1672-352x.20240313.006

降解氨基甲酸乙酯酿酒酵母的筛选及其应用

Screening of ethyl carbamate degraded brewer's yeast and its application

夏尚帆 1李奎 1王文武 1张惠玲1
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作者信息

  • 1. 宁夏食品微生物应用技术与安全控制重点实验室,银川 750021;宁夏大学食品科学与工程学院,银川 750021
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摘要

以宁夏5个产区的酿酒葡萄为原料筛选可以降解氨基甲酸乙酯(Ethyl carbamate,EC)且具有优良酿酒特性的红葡萄酒和白葡萄酒酿酒酵母.初筛试验获得了10株酵母,复筛试验以EC作为唯一氮源筛选出6株EC降解能力较好的酵母菌株,最后通过产气试验和耐受性试验筛选出了2株发酵性能较好的菌株.经过18S rDNA分子生物学鉴定后确定这2个菌株为酿酒酵母.使用筛选出的2个菌株发酵红葡萄酒和白葡萄酒,并以商业酵母作为对照,测定其理化指标及EC含量;再通过GC-MS分析其香气物质并结合感官评价最终筛选出具有优良酿酒特性和降解EC功效的红葡萄酒酿酒酵母(Saccharomyces cerevisiae)NXSC2和白葡萄酒酿酒酵母(Saccharomyces cerevisiae)NXSC1.

Abstract

Red and white wine yeasts with excellent winemaking characteristics were screened for ethyl car-bamate (EC) degradation using wine grapes from five production areas in Ningxia as materials. Ten yeast strains were obtained in the initial screening experiment, of which, six strains with good EC degradation ability were ob-tained in the re-screening experiment using EC as the only nitrogen source, and finally two strains with good fer-mentation performance were obtained through gas production and tolerance experiments. After 18S rDNA molec-ular biology identification, these two strains were identified as Saccharomyces cerevisiae. The two screened strains were used to ferment red and white wines, and commercial yeasts were used as controls to measure their physicochemical indexes and EC content; the aroma substances were then analyzed by GC-MS combined with sensory evaluation to finally screen out red wine brewing yeast(S. cerevisiae) NXSC2 and white wine brewing yeast (S. cerevisiae)NXSC1, which had the excellent winemaking characteristics and EC degrading effect.

关键词

氨基甲酸乙酯/菌种筛选/红葡萄酒/白葡萄酒/菌种鉴定

Key words

ethyl carbamate/yeast strains screening/red wine/white wine/strain identification

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基金项目

&&(2020BFH02006)

出版年

2024
安徽农业大学学报
安徽农业大学

安徽农业大学学报

CSTPCDCSCD
影响因子:0.412
ISSN:1672-352X
参考文献量25
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