Effects of NaCl stress on seed germination and seedling physiological characteristics of Digitaria sanguinalis
The purpose of this paper is to clarify the effects of NaCl stress on seed germination and physio-logical characteristics of Digitaria sanguinalis,and to predict and comprehensively manage the suitable area of D. sanguinalis in direct seeding rice fields. The seeds of D. sanguinalis were cultured in 0,10,30,60,100,150 and 300 mmol·L-1 NaCl solution,the germination indexes of D. sanguinalis seeds were determined,and the seedling biomass,chlorophyll content,malondialde-hyde (MDA) content,root activity,superoxide dismutase (SOD) activity and peroxidase (POD) activity were compared. The results showed that with the increase of NaCl concentration,the seed germination rate,relative germination rate,germination potential,germination index,radicle and germ length showed a continuous downward trend,and the relative salt injury index increased gradually. When the concentration reached 300 mmol·L-1,the seed stopped germination;when NaCl concentration was 60 mmol·L-1,the radicle and germ length of D. sanguinalis reached the control level. The fresh quality of seedlings decreased with the increase of NaCl concentration,and there was no significant difference in dry quality of seedlings compared with the control (P<0.05). The chlorophyll content of the seedlings gradually decreased with the increase of NaCl concentration. At NaCl concentration of 60 mmol·L-1,the root activity and SOD activity increased compared with the control. MDA content increased under NaCl treatment compared with the untreated control,and it increased significantly when NaCl concentration was 300 mmol·L-1 (P<0.05). The results indicated that the germination of D. sanguinalis has a wide range of adaptation to NaCl stress,and the seedlings have strong salt tolerance.
万方数据
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