解淀粉芽胞杆菌SJ1606产脂肽粗提物协同代森锰锌对2种植物病菌的抑制效果

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中文摘要：为探明微生物源脂肽粗提物、代森锰锌及二者的复配剂对黄瓜枯萎病菌和葡萄白腐病菌的抑制效果,采用菌丝生长速率法、Horsfall法和Wadley公式法检测了脂肽粗提物和代森锰锌对2种病菌的抑制率和增效比.脂肽粗提物对黄瓜枯萎病菌的EC50为1.93μL·mL-1,对葡萄白腐病菌的EC50为5.54μL·mL-1,代森锰锌对黄瓜枯萎病菌的EC50为74.37 mg·L-1,对葡萄白腐病菌的EC50为48.45 mg·L-1.Horsfall法得出脂肽与代森锰锌混配体积比为8∶2对黄瓜枯萎病菌具显著的增效活性,体积比为1∶9对葡萄白腐病菌具有显著增效活性.通过Wadley公式法得出混配组合对黄瓜枯萎病菌理论EC50为1298.78 mg·L-1,实际EC50达472.48 mg·L-1,增效比(SR)为2.75;对葡萄白腐病菌理论EC50值为481.41 mg·L-1,实际EC50值187.32 mg·L-1,增效比(SR)为2.57.结果为2种不同病害的持续防控、化学药剂的减施增效和抗菌脂肽的合理应用提供支撑.

外文标题：Inhibitory effect of crude lipopeptide extracts from Bacillus amyloliquefaciens SJ1606 combined with mancozeb on two plant pathogens

外文摘要：In order to investigate the inhibitory effects of microbial crude lipopeptide extracts,mancozeb and their mixture on Fusarium oxysporum f. sp. cucumerinum and Coniella diplodiella,the inhibition rate and synergistic ratio of crude lipopeptide extracts and mancozeb on the two pathogens were detected by mycelial growth rate meth-od,Horsfall method and Wadley formula method. The results showed that the EC50 of crude lipopeptide extracts against F. oxysporum f.sp. cucumerinum was 1.93 μL·mL-1,the EC50 of crude lipopeptide extracts against C. Diplodiella was 5.54 μL·mL-1 and EC50 of mancozeb against F. Oxysporum f. Sp. Cucumerinum and C. Diplodiella were 74.37 and 48.45 mg·L-1,respectively. The results of Horsfall method showed that the volume ratio of lipopep-tide to mancozeb was 8∶2,which had significant synergistic activity against F. Oxysporum f. Sp. Cucumerinum,and the volume ratio was 1∶9,which had significant synergistic activity against C. Diplodiella. According to the Wadley formula method,the theoretical EC50 of the mixture against F. Oxysporum f.sp. Cucumerinum was 1298.78 mg·L-1,the actual EC50 was 472.48 mg·L-1,and the synergistic ratio (SR) was 2.75;the theoretical EC50 value against C. Diplodiella was 481.41 mg·L-1,the actual EC50 value was 187.32 mg·L-1,and the synergistic ratio (SR) was 2.57. The results provide some supports for the sustainable prevention and control of two different diseases,the reduction of chemical agents and the rational application of antimicrobial lipopeptides.

外文关键词：

cucumber Fusarium wiltgrape white rotcrude lipopeptide extractsMancozebcombination

作者：

王猛、吴含、段海明、孟祥涛、李之硕

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作者单位：

安徽科技学院农学院,凤阳 233100

关键词：

黄瓜枯萎病葡萄白腐病脂肽粗提物代森锰锌混配

基金：

安徽省自然科学基金安徽省高等学校自然科学研究项目安徽省高等学校自然科学研究项目作物抗逆育种与减灾国家地方联合工程实验室开放基金安徽省重点研发计划国家级大学生创新训练项目

项目编号：

2308085MC90KJ2017A510KJ2019A0814NELCOF20190102202104a06020001202210879055

出版年：

2024

DOI：

10.13610/j.cnki.1672-352x.20240510.016

安徽农业大学学报

安徽农业大学

安徽农业大学学报

CSTPCD

影响因子：0.412

ISSN：1672-352X

年,卷(期)：2024.51(2)

参考文献量41