Study on the optimization of rapid propagation system and the effect of detoxification of sweet potato stem tip
To solve the problem of sweet potato virus disease,detoxification of sweet potato was carried out by means of stem tip detoxification,and the effects of different disinfectants and disinfection time on the disinfec-tion effect and virus-free rate of sweet potato explant were studied.The virus species carried by the sweet potato seedlings were detected by RT-PCR;the virus detected was deviralized by stem tip culture,and the effects of dif-ferent disinfectants and disinfection time on the disinfection effect of sweet potato explants were conducted;addi-tionally,to optimize the rapid propagation system of sweet potato,the effects of different hormone concentration on shoot apex induction and root induction were explored by a orthogonal experiment;finally,the detoxification effect of stem tip was detected by RT-PCR.As results,the primary detection of sweet potato virus showed that sweet potato mainly carried SPVG,SPFMV and SPCSV viruses,among which SPVG carried the highest virus rate;the appropriate disinfection method for stem tip induction of explants was:70%alcohol for 45 s,and then immersed in 0.1%Hg solution for 7-8 min;the optimal medium for stem tip induction was MS+1.0 mg·L-1 6-BA+0.1 mg·L-1NAA+1.0 mg·L-1GA3,and the optimal medium for the root induction was 1/2 MS+0.1 mg·L-1NAA+0.1 mg·L-1 IAA.After shoot tip detoxification,SPFMV had the best detoxification rate(100%),fol-lowed by SPVG,and SPCSV had the worst detoxification rate(about 3.33%).
sweet potatostem tip detoxificationvirus detectionrapid propagationshoot apex tissue culture
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