首页|中华蜜蜂泛素基因及其全长转录本的发掘及分析

中华蜜蜂泛素基因及其全长转录本的发掘及分析

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
旨在利用三代Nanopore测序技术发掘中华蜜蜂(Apis cerana cerana)泛素(ubiquitin)基因及其全长转录本.基于前期获得的中华蜜蜂工蜂 4～6 日龄幼虫肠道的全长转录组数据,使用BLAST工具将全长转录本的序列比对到 KEGG 和 Nr 数据库以鉴定泛素基因及其全长转录本.采用 Astalavista 软件分析泛素基因的可变剪接(alternative splicing,AS)事件,并通过RT-PCR加以验证.通过TAPIS pipeline软件分析泛素基因的可变多聚腺苷酸化(alternative polyadenylation,APA)位点.共鉴定到 48个中华蜜蜂泛素基因和 435条泛素基因相关全长转录本.共发掘到 48个泛素基因的 74次AS事件,包括 31次内含子保留事件,19次可变 3'端剪接事件,16次可变5'端剪接事件及 8次外显子互斥事件.RT-PCR结果证实了 3种AS事件类型的真实性.共预测到 38个泛素基因含有 1个及以上的APA位点,并且在APA位点的上游鉴定到多个motif,一致性序列为:KCWYTDYTMWSYGMW SCARAWCCAGAATGAYCCWYWGGHWVMWGWDRTRGC.研究结果丰富了中华蜜蜂泛素基因及其全长转录本信息,为持续深入开展相关功能研究提供参考和依据.
Exploration and investigation of Apis cerana cerana ubiquitin genes and their full-length transcripts
This study aimed to explore ubiquitin genes and their full-length transcripts in Apis cerana cerana using three-generation Nanopore sequencing technology.Based on the previously obtained full-length transcrip-tome data of the intestinal tract of 4-6-day-old worker larvae of Apis cerana cerana,the sequences of full-length transcripts were aligned to KEGG and Nr databases using BLAST tools to identify ubiquitin genes and their full-length transcripts.Astalavista software was used to analyze alternative splicing(AS)events of ubiquitin genes and verified by RT-PCR.Additionally,the TAPIS pipeline software was employed to analyze alternative polyad-enylation(APA)sites of ubiquitin genes.A total of 48 ubiquitin genes of Apis cerana cerana and 435 ubiquitin gene related full-length transcripts were identified.A total of 74 AS events of 48 ubiquitin genes were identified,including 31 intron retention events,19 variable 3′-end splicing events,16 variable 5′-end splicing events and 8 exon mutual exclusion events.RT-PCR results confirmed that the three randomly selected AS event types were real and reliable.Furthermore,38 ubiquitin genes were forecasted to contain one or more of the APA site,and in the upstream of the APA site identification to multiple motifs,consistency sequence was:KCWYTDYTMWSYG MWSCARAWCCAG AATGAYCCWYWGGHWVMWGWDRTRGC.The results of this study enrich the data-base of ubiquitin genes and their full-length transcripts information of Apis cerana cerana,and provide resource and foundation for further research on related functions.

Apis ceranaApis cerana ceranafull-length transcriptubiquitinalternative splicingalterna-tive polyadenylation

刘小玉、张佳欣、高旭泽、冯佩林、蔡宗兵、那志豪、陈大福、郭睿、徐国钧

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福建农林大学蜂学与生物医药学院,福州 350002

天然生物毒素国家地方联合工程实验室,福州 350002

福建省蜂疗研究所,福建 350002

东方蜜蜂 中华蜜蜂 全长转录本 泛素 可变剪接 可变多聚腺苷酸化

国家自然科学基金项目国家自然科学基金项目国家现代农业产业技术体系专项资金项目福建省自然科学基金面上项目福建农林大学硕士生导师团队项目(郭睿)福建农林大学科技创新专项基金项目(郭睿)宁夏回族自治区重点研发项目福建农林大学动物科学学院(蜂学学院)科研扶持项目

3217279231702190CARS-44-KXJ72022J011313342022BBF02037

2024

10.13610/j.cnki.1672-352x.20240710.021

安徽农业大学学报
安徽农业大学

安徽农业大学学报

CSTPCD
影响因子：0.412
ISSN：1672-352X
年,卷(期)：2024.51(3)
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