Isolation and identification of Vibrio cholerae,a pathogen of Procambarus Clarkii
A dominant strain,LK-18,was isolated from the lesion tissue of the tail fan of Procambarus clarkia,and its species identification and pathogenicity analysis of the strain was conducted.Preliminary bacteria classification was determined through observation of colony morphology,16S rRNA sequencing,serotype analysis and gene char-acterization.The pathogenicity of LK-18 was assessed using artificial infection tests,which included immersion infec-tion,intramuscular injection and intraperitoneal injection.After 16S rRNA sequencing analysis and four housekeeping gene(ATP synthase alpha,atpA;uridylate kinase gene,pyrH;recombinase A gene,recA;DNA gyrase subunit B gene,gyrB)concatenation analysis,the strain was ultimately identified and named as V.cholerae LK-18.V.cholerae LK-18 was identified by PCR as not belonging to the O1 and O139 serotypes but containing ctx virulence gene and chitinase(Chi)gene related to chitin degradation.Although the artificial infection tests failed to reproduce the symptoms of rot-ting tail,V.cholerae LK-18 isolated from the rotting tail tissue could cause mortality in P.clarkii,suggesting its poten-tial pathogenicity.The results of this study indicated that V.cholerae LK-18 is a pathogenic bacteria,which not only broadens the known infected host range of O1/O139 group V.cholerae,but also raises concerns about the potential for disease outbreaks in the P.clarkii aquaculture industry.Therefore,it is necessary to take preventive measures in ad-vance to mitigate the risks of V.cholerae infection and to avoid associated losses.
Procambarus clarkiipathogenic bacteriaVibrio choleraeisolation and identification
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