In order to solve the environmental pollution caused by organic matter and phosphorus in the biogas slurry of pig manure and to improve the generation of clean energy,the effects of zero-valent iron on methane pro-duction and phosphorus transformation were investigated during the anaerobic digestion of pig manure in this study.Techniques such as Fourier transform infrared spectrometer(FTIR),X-ray diffraction(XRD),and high-throughput sequencing were used to analyze the changes in methane production,phosphorus distribution and species,enzyme activities,and microbial community during anaerobic digestion.The results showed that zero-valent iron enriched acidifying bacteria such as Clostridun_sensu_stricto_1,Terripotobacter and Syntrophomonas,and the activities of protease and β-glucosidase were increased by 0.6 times and 2.4 times,respectively after adding zero-valent iron,and strengthened the hydrolytic acidification ability of the system.Moreover,zero-valent iron enriched Methanosaeta,which was help to enhance the activity of methanogen,and thus the methane production increased by 11.1%.In ad-dition,zero-valent iron also enriched phosphorus transforming microorganisms,such as Ruminofilibacter,WS6_Doj kabacteria etc.,which increased the activities of acid(alkali)phosphatase by about 26.3%,and promoted the conversion of organic phosphorus to inorganic phosphorus in the system.Moreover,zero-valent iron significantly increased the content of soluble Fe2+in the system,and improved the conversion of water-soluble phosphorus to Fe-bound phosphorus,which was finally fixed in the precipitate such as vivianite,and thus significantly reduced the concentration of total phosphorus in biogas slurry.The results provide a technical reference for improving methane production and phosphorus fixation in anaerobic digestion of pig manure.
pig manureanaerobic digestionzero-valent ironphosphorusmethane production
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