Dynamic changes of bacterial microecology in casing soil during the cultivation of Agaricus bisporus
Microorganisms,particularly bacteria,play a crucial role in the casing soil used for cultivating Agari-cus bisporus,significantly affecting the growth and development of both mycelia and fruiting bodies.The advanced and precise 16S rDNA technique was routinely employed to monitor and assess the dynamic changes in bacterial eco-logical communities within casing soils during various growth stages of Agaricus bisporus,thereby investigating the underlying patterns.Throughout all stages,Proteobacteria emerged as the dominant phylum,constituting 38.67%-47.93%of the microbial composition;Bacteroidetes followed closely,accounting for 18.13%-37.80%of the total abundance.The primary bacterial composition remained relatively stable,predominantly comprising Chryseobacte-rium,Sphingomonas,Flavobacterium,Comamonas,and Arthrobacter.Chryseobacterium,etc..The genus with the highest overall abundance proportion,displayed a lower level in the casing soils on day 0,however,its abundance sig-nificantly increased after four days and then exhibited a declining trend after eight days,eventually stabilizing before the end of cultivation.The involved growth process of A.bisporus could be divided into three stages:day 0,day 4-17,and after day17 according to the PLS-DA and LefSe analysis.The abundance of Phenylobacterium in day 4-17 was higher,while the unclassified_Rhizobiales at day 0 was relatively higher.Among all bacterial genera,Phenylobacte-rium exerted the most significant impact on the microbial community within the casing soils.
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