摘要
为研究不同产地 C2F等级烤烟表面细菌微生物的组成和结构,利用高通量测序技术测试了 6 个不同产地的C2F等级烤烟在醇化初期烟叶表面可培养细菌的组成和结构.结果表明:6个不同产地的烟叶样品表面分别检测到 55、39、33、26、24和 15个OTU,其中 6个烟叶样品共同的OTU为 6个.在属分类水平上,大部分烟叶表面可培养的优势细菌微生物是芽孢杆菌属(Bacillus),部分烟叶表面活跃着肠杆菌属(Enterobacter),伯克霍尔德菌属(Burkholderia)和罗尔斯通菌属(Ralstonia).不同品种和产地的烤烟表面细菌结构具有明显差异,同一品种不同产地的烤烟表面细菌结构也存在明显差异.研究结果为利用细菌微生物醇化不同产区烟叶,提高烟叶品质、优化烟叶醇化流程提供理论依据.
Abstract
In order to study the composition and structure of bacteria on the surface of C2F flue-cured tobac-co from different regions,the composition and structure of culturable bacteria on the surface of tobacco leaves from 6 different regions at the early stage of aging were tested using high throughput sequencing technology.The results showed that 55,39,33,26,24 and 15 OTUs were detected on the surface of tobacco samples from six dif-ferent origins,respectively,and 6 OTUs were shared by tobacco samples from six different origins.At the genus classification level,the dominant bacterial microorganism that could be cultured on the surface of most tobacco leaves was Bacillus,and Enterobacter,Burkholderia and Ralstonia were active in some flue-cured tobacco sur-faces.There were obvious differences in the types of bacteria on the surface of flue-cured tobacco leaves from different varieties and producing areas,and also from different producing areas of the same variety.The research results provide a theoretical basis for using bacteria to age tobacco leaves from different producing areas,improve the quality of tobacco leaves,and optimize the tobacco leaf aging process.
基金项目
安徽中烟工业有限责任公司科技项目(2020121)
国家科技期刊平台
NSTL
万方数据