60Co-γ radiation breeding is the most widely used mutagenesis technology in the current breeding research,which has made great contributions to the development of agriculture in China.However,there are few applications in the breeding of woody plants.Many studies are difficult to form breakthrough research outputs limited by the long growth cycle of woody plants.Therefore,systematic and comprehensive theoretical guidance is urgently needed.In this paper,the research on 60Co-γ radiation mutagenesis breeding of woody plants in the past 20 years was summarized,and the principles and methods of radiation mutagenesis,the selection of radiation materials and radiation intensity,and the identification of mutants in detail were introduced.Besides,the effects and molecular mechanisms of 60Co-γ radiation mutagenesis were discussed,and the key directions of follow-up research were proposed,which could provide a theoretical guidance for 60Co-γ radiation mutagenesis breeding of woody plants in the future.
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