Effects of low temperature on stress level and innate immunity in juvenile Mauremys reevesii
In order to explore the physiological adaptation to low temperature threatens in juvenile Chinese three-keeled pond turtle,Mauremys reevesii,the variations of body size,stress level,and plasma bactericidal ca-pacity were measured on day 1,day 10 and day 28,respectively,under the conditions of 6,17 and 26 ℃ by using of dorsal cervical sinus blood sampling technique.The results were as follows.(1) Both different temperatures and treatment time had no great effects on the body mass,the length and width of carapace in juvenile turtles (P>0.05).(2) The ratio of heterophils to lymphocytes did not change with treatment time at any temperature group (P>0.05),only on day 1,it was significantly lower at 6 ℃ than that at 17 ℃ (P<0.05).(3) With prolonged treatment time,plasma bactericidal capacity was greatly declined at 17℃ group (P<0.05),but it was signifi-cantly increased at 26 ℃ group (P<0.05),only on day 28,it was significantly lower at the groups of 6 ℃ and 17 ℃ than that of 26 ℃ group (P<0.05).Lower temperatures have no marked inhibitory effect on the growth of juvenile turtles,short-term cold temperature threatens produce stronger stress response,accompanying with sig-nificantly reduced innate immunity,which are not beneficial for the juvenile turtles to adapt to the large-scale fluctuation of environmental temperature before hibernation and post-hibernation.
万方数据
维普
