摘要
为研究高寒地区藏羊胃肠道菌群的特点,利用宏基因组测序技术分析青海省祁连县藏羊胃肠道微生物的组成及其多样性,对该地区藏羊肠道菌群进行了细菌多样性、丰度及功能注释的检测.结果表明:在古生菌门水平上QL地区藏羊胃肠道的优势菌门为Euryarchaeota,在属水平的优势菌属为Methanobrevibacter;在细菌门水平上,QL地区藏羊瘤胃的优势菌门为Bacteroidetes,而肠道的优势菌门均为Firmicutes,属水平上,QL地区藏羊瘤胃的优势菌属为Prevotella,回肠优势菌属为Clostridium,盲肠优势菌属为Bacteroides;在真菌门水平上,QL地区藏羊胃肠道的优势菌门为Ascomycota,在属水平上瘤胃的优势菌属为Mucor,回肠优势菌属为Paraphaeosphaeria,盲肠优势菌属为Colletotrichum.在CAZyme、eggNOG、KEGG分析中,发现肠道微生物代谢功能富集主要在糖苷水解酶、碳水化合物运输与代谢、氨基酸运输与代谢、核苷酸代谢等;在CARD的抗性基因分析中,QL藏羊胃肠道在Bifidobacterium注释到的基因丰度最多,其次为Nocardia、MexF.上述结果将为进一步研究高海拔高寒地区反刍动物肠道微生物的作用机理奠定基础.
Abstract
In order to investigate the characteristics of gastrointestinal flora of Tibetan sheep in alpine regions,this study analyzed the diversity of gastrointestinal microorganisms of Tibetan sheep in Qilian country,Qinghai Province,by macro-genome sequencing technology.The results showed that the dominant phylum in the gastrointes-tinal tract of Tibetan sheep in the QL region was Euryarchaeota at the level of Archaea,and the dominant genus at the level of genera was Methanobrevibacter.At the bacterial phylum level,the dominant phylum in the rumen of Tibetan sheep in the QL region was Bacteroidetes,while the dominant phylum in the intestinal tract was Firmicutes in all cases,and at the genus level,the dominant genus in the rumen of Tibetan sheep in the QL region was Prevotella,the dominant genus in the ileum was Clostridium,and the dominant genus in the cecum was Bacteroides.At the fungal phylum level,the dominant phylum in the gastrointestinal tract of Tibetan sheep in the QL region was Ascomycota,and at the genus level,the dominant phylum in the rumen was Mucor,the dominant phylum in the ileum was Para-phaeosphaeria,and the dominant phylum in the cecum was Mucor.In the CAZyme,eggNOG,and KEGG analyses,the metabolic functions of intestinal microorganisms were found to be enriched mainly in the following areas,such as glycoside hydrolases,carbohydrate transport and metabolism,amino acid transport and metabolism,and nucleo-tide metabolism.Additionally,resistance gene profiling via CARD analysis demonstrated that the gastrointestinal tract of QL Tibetan sheep exhibited the highest abundance of genes annotated in Bifidobacterium,followed by No-cardia and MexF.These comprehensive findings provide a foundation for further research on the mechanism of ac-tion of gut microorganisms of ruminants in high altitude and high alpine regions.
维普
万方数据