Diversity of the gastrointestinal flora of Tibetan sheep in Qilian region revealed by macrogenomics-based technology
In order to investigate the characteristics of gastrointestinal flora of Tibetan sheep in alpine regions,this study analyzed the diversity of gastrointestinal microorganisms of Tibetan sheep in Qilian country,Qinghai Province,by macro-genome sequencing technology.The results showed that the dominant phylum in the gastrointes-tinal tract of Tibetan sheep in the QL region was Euryarchaeota at the level of Archaea,and the dominant genus at the level of genera was Methanobrevibacter.At the bacterial phylum level,the dominant phylum in the rumen of Tibetan sheep in the QL region was Bacteroidetes,while the dominant phylum in the intestinal tract was Firmicutes in all cases,and at the genus level,the dominant genus in the rumen of Tibetan sheep in the QL region was Prevotella,the dominant genus in the ileum was Clostridium,and the dominant genus in the cecum was Bacteroides.At the fungal phylum level,the dominant phylum in the gastrointestinal tract of Tibetan sheep in the QL region was Ascomycota,and at the genus level,the dominant phylum in the rumen was Mucor,the dominant phylum in the ileum was Para-phaeosphaeria,and the dominant phylum in the cecum was Mucor.In the CAZyme,eggNOG,and KEGG analyses,the metabolic functions of intestinal microorganisms were found to be enriched mainly in the following areas,such as glycoside hydrolases,carbohydrate transport and metabolism,amino acid transport and metabolism,and nucleo-tide metabolism.Additionally,resistance gene profiling via CARD analysis demonstrated that the gastrointestinal tract of QL Tibetan sheep exhibited the highest abundance of genes annotated in Bifidobacterium,followed by No-cardia and MexF.These comprehensive findings provide a foundation for further research on the mechanism of ac-tion of gut microorganisms of ruminants in high altitude and high alpine regions.
万方数据
维普
