Research on aroma components in flowers and leaves of five cultivars of Cannabis sativa
In order to determine the aroma components in flowers and leaves of different C.sativa cultivars,five female cultivars including'brosom'('Bs'),'cherry'('Cr'),'hemp7'('H7'),'hanma'(Hm)and'zhongxian 1'('Zx1')under the same cultivation conditions at full flowering stage were used as materials,and the aroma components in the flowers and leaves were detected by HS-GC/MS(head space-gas chromatography/mass spectrometry)technology.The results showed that the flowers and leaves aroma components of the five cultivars had their own characteristics.A total of 43 kinds of flowers aroma substances with relative content of more than 0.1％were detected in the five culti-vars,and 30,7,28,25 and 19 kinds of flowers aroma components were identified in'Bs','Cr','H7','Hm'and'Zx1',respectively.Monoterpenes were the main components,with the highest relative content(98.15％)of monoterpenoids in the'Cr'flowers,the highest sesquiterpene content(27.51％)in the'Bs'flowers,and the dominant component ofα-pinene in the'Cr','Hm'and'Zx1'.Atotal of 51 kinds of leaves aroma substances were detected in the five cultivars,33,19,31,26 and 31 kinds of leaves aroma components were identified in'Bs','Cr','H7','Hm'and'Zx1',respec-tively.The contents of sesquiterpene(59.24％)and alcohol(11.03％)in the leaves of'Bs'were significantly higher than those in other four cultivars.The contents of monoterpenoids(84.91％)and esters(8.64％)in the leaves of'Cr'were significantly higher than those in the other cultivars.According to their aroma similarity and cluster analysis,the aroma similarity rate of'Hm'and'Zx1'was the highest(0.995),and'Bs'aroma components composition was the most unique,fell into one group.The differential expression of α-Pinene,β-Pinene,D-Limonene,caryophyllene andβ-Farnesene in the flowers and leaves of C.sativa enriched the basic knowledge on aroma components.
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