Study on the contents and activities of polyphenols and flavonoids in by-products of Camellia oleifera Abel.
To determine the contents of polyphenols and flavonoids in Camellia oleifera Abel.,three dif-ferent solvents(pure water,50%ethanol,95%ethanol)were used to extract its different parts(leaves,shells and cake)by ultrasonic extraction,the contents of polyphenols and flavonoids of each extract were determined,the antioxidant activity was evaluated by DPPH free radical scavenging ability and ORAC antioxidant ability index,and the tyrosinase inhibitory activity and cell proliferation activity were studied.The contents of poly-phenols and flavonoids in C.oleifera Abel.were higher than those in C.oleifera Abel.cake and C.oleifera Abel.shell,and the contents of polyphenols and flavonoids extracted from 50%ethanol were the highest,which were(337.24±1.94)and(189.23±1.12)mg·g-1,respectively.The IC50 of scavenging DPPH free radicals was(28.10±0.46)pg mL-1,the index of ORAC antioxidant capacity was(2 651.54±112.41)μmol·g-1,and the IC50 of tyrosinase inhibition rate was(140.20±0.95)μg·mL-1.