Identification and pathogenecity of rot fungal pathogens of Castanea mollissima var.Yanshanzaofeng
Castanea mollissima var.Yanshanzaofeng,a significant chestnut variety in China,is perennially affected by a complex fruit/seed rot disease,whose pathogenic composition and virulence have remained unclear.In this study,Koch's postulates were employed to isolate and identify the causative pathogens,combining molec-ular phylogenetic analysis with morphological characteristics.Furthermore,the pathogenicity of fungal conidia,the virulence of endophytic fungi within chestnuts,and the impact of temperature on pathogenicity were evaluated.The results indicated that the pathogens responsible for fruit/seed rot in'Yanshanzaofeng'chestnuts were distrib-uted across several genera,including Botryosphaeria,Diaporthe,Fusarium,Trichothecium,Colletotrichum,Al-ternaria and Lasiodiplodia.Among these,B.dothidea,D.nobilis and Lasiodiplodia species were identified as the primary pathogenic species.It was found that lower temperatures significantly affected the virulence of these pathogens and reduced the diversity of pathogenic species.The conidia of these fungi were determined to be pathogenic,and strains of Diaporthe and Alternaria endophytic fungi within the chestnut also exhibited patho-genicity.This study systematically revealed,for the first time,the pathogenic structure and virulence of a fruit/seed rot disease in a chestnut variety,which lay a crucial foundation for potential exploration and the estab-lishment of effective prevention and control strategies and techniques.
chestnutvariety of Yanshanzaofengfruit rot diseasespathogensidentification
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