Identification and expression analysis of RsSnRK gene family in Rhododendron simsii
In order to explore the response of sucrose non-fermenting-1-related protein kinase(SnRK)to abiotic stress in Rhododendron simsii,based on R.simsii genome data and bioinformatics technology,RsSnRKs were screened,identified and analyzed,and real-time quantitative PCR(RT-qPCR)was used to detect the response of the family genes to drought stress.The results showed that a total of 19 members from 3 subfamilies of the R.simsii RsSnRK gene family were identified.The encoded proteins were all hydrophilic proteins with a length of 362 to 555 amino acids,with an isoelectric point of 4.80 to 9.27 and a relative molecular mass being 41 025.65-62 951.44 Da.The secondary structures were dominated by α-helices and irregular coil.It was predicted that they were mainly located in the cell nucleus or cytoplasm.Motif and cis-acting element analysis found that genes of the same subfamily of RsSnRKs had similar structures and motifs,and 2 000 bp upstream bases in RsSnRKs containing a large number of light response,hormone response,and abiotic stress response elements.Collinearity analysis found that the evolution-ary selection pressure values of four groups of collinear pairs of genes,including Rhododendron RsSnRK2/RsSnRK14,were all less than 1 and were subject to purifying selection;10 RsSnRKs formed 19 collinearity relationships with 15 AtSnRKs.RT-qPCR analysis found that the relative genes expression of RsSnRK7,RsSnRK9 and RsSnRK13 were sig-nificantly upregulated with drought,and significantly downregulated after rehydration,which indicated that RsSnRKs in R.simsii responded actively to drought stress.The research result lay a foundation for further exploring the function of RsSnRKs genes and provides a basis for screening drought resistant genes in Rhododendron.
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