Analysis of the transcriptome and SSR loci in the larvae of Illiberis pruni
In order to explore the distribution characteristics of SSR(simple sequence repeat)loci and thereby lay a foundation for the development and enrichment of novel EST-SSR-molecular markers,the larvae of the pear star moth were subjected to transcriptome sequencing using the Illumina HiSeqTM2000 high-throughput sequenc-ing platform.On this basis,microsatellite loci were screened and their distribution characteristics were analyzed.The results showed that a total of 52 671 transcripts and 21 432 unigenes were obtained from the transcriptome sequencing of Illiberis pruni.The dominant categories in the COG,GO annotations,and KEGG pathway database annotations of unigenes were general function prediction,cell process transport and catabolic.A total of 8 288 microsatellite loci were distributed in 5 832 unigenes.The main types of SSR repeats were mononucleotide and dinucleotide repeats.Among the mononucleotide repeats,the primary motif type was(A/T)n,accounting for 77.94％of the total SSR loci.The dinucleotide repeat(AC/GT)n was the dominant motif,representing 2.38％of the total SSR loci.The number of SSRs in each maternal blood sequence decreased with an increase in the base repeat types,showing an inverse relationship.In the analysis of the proportion of transcript chips from each type,the proportion of transcript chips of the four motifs of the SSR dinucleotide repeat types was the highest(48.03％),with the AT/AT motif being the most abundant(228 transcripts).The research findings facilitated the successful screening of microsatellite loci of Illiberis pruni based on transcriptome data,providing a research foundation for the genetic diversity analysis and gene mining of Illiberis pruni.
国家科技期刊平台
NSTL
万方数据
