The effect of the daidzein on the levels of several endogenous hormones in the blood of ruminants and explore its possible mechanism was explored. With 4 local non-pregnant female goats at 1-year-old as test objectives, the experiment was divided two periods of control period (daily perfusing 5 ml physiological saline by duodenal fistula) and test period (daily perfusing 5ml daidzein physiological saline suspension containing 200 mg daidzein ), with each period of 12 days. Contents of GH, T3, T4 and 17-β estradiol in goat serum were determined by radioimmunoassay method.The results showed that the levels of T3,T4,GH and E2 in blood were increased significantly by duodenal infusion of daidzein,being higher 128.57%, 124.12%, 106.49% and 72.34% than those in control period,indicating that daidzein could regulate the growth and nominal digestion and metabolism of ruminants indirectly through regulating the levels of endogenous hormones. The research provided a theoretical basis to study and develop new botanical animal growth promoters which were not harmful and easy to be popularized and applied.